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Cold-water-fish probiotics bacillus strain and application thereof

A technology of bacillus and cold-water fish, applied in the field of microbiology, can solve the problem of low antibody titer

Inactive Publication Date: 2015-12-09
哈尔滨国生生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish have the ability of immune memory response. If you want to obtain a strong immune response again, it will take a long time, and the antibody titer produced by the secondary response is lower than that of mammals.

Method used

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  • Cold-water-fish probiotics bacillus strain and application thereof
  • Cold-water-fish probiotics bacillus strain and application thereof
  • Cold-water-fish probiotics bacillus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Isolation, purification and identification of bacillus HZC58 of the present invention

[0032] GM17 solid medium formula (g / L):

[0033] Soytone 5.0, peptone 2.5, casein peptone 2.5, yeast extract powder 2.5, beef powder 5.0, lactose 5.0, sodium ascorbate 0.5, β-sodium glycerophosphate 19.0, magnesium sulfate 0.25, agar 15, after dissolving, sterilize at 110°C for 15 minutes, Adjust the pH to 7.2 and pour the plate for later use.

[0034] GM17 liquid medium formula (g / L):

[0035] Soytone 5.0, peptone 2.5, casein peptone 2.5, yeast extract powder 2.5, beef powder 5.0, lactose 5.0, sodium ascorbate 0.5, β-sodium glycerophosphate 19.0, magnesium sulfate 0.25, after dissolution, sterilize at 110°C for 15 minutes, adjust pH 7. 2, spare.

[0036] (1) Separation and purification of bacterial strains:

[0037] Juvenile rainbow trout from the semi-wild mountainous area of ​​Benxi, Liaoning Province should minimize the stress of rainbow trout, quickly disinfect ...

Embodiment 2

[0043] Embodiment 2 The acid resistance characteristic research of bacillus HZC58 bacterial strain of the present invention

[0044] The unique acid resistance properties of this Bacillus strain HZC58:

[0045] (1) Experimental method: the Bacillus HZC58 strain was cultured at 30°C, and the growth rate of the strain in GM17 liquid medium with different pH (pH3.0, pH4.0, pH5.0, pH6.0 and pH6.5) was observed. growing situation.

[0046] (2) Experimental results: the bacillus HZC58 bacterial strain has good adaptability in the culture medium of different pH, and it can proliferate in the acidic GM17 medium when the pH is 4.0; Proliferate in the slightly acidic GM17 medium that is 5.0 in pH Fast; proliferates rapidly in near-neutral GM17 medium with a pH value of 6.5. See figure 2 The proliferation curve indicated that the Bacillus HZC58 has the ability to tolerate the low pH environment of the intestinal tract, indicating that the Bacillus HZC58 strain has the ability to colo...

Embodiment 3

[0047] Embodiment 3 Research on the cholate-resistant characteristic of Bacillus bacillus HZC58 bacterial strain of the present invention

[0048] The unique cholate resistance properties of this Bacillus sp. HZC58 strain:

[0049] (1) Experimental method: the Bacillus HZC58 bacterial strain was cultured at 30°C, and the observed strain was cultured in GM17 liquid containing different cholate concentrations (cholate concentrations: 0.10%, 0.20%, 0.30%, 0.40% and 0.50%) growth in the base.

[0050] (2) Experimental results: rapid proliferation can be achieved in GM17 medium containing 0.0%-0.2% cholate; rapid proliferation can still be achieved in GM17 medium containing 0.3-0.5% cholate. See image 3 The proliferation curve of Bacillus sp. HZC58 showed that the strain Bacillus HZC58 had the ability to colonize in intestinal hypercholate.

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Abstract

The invention discloses a cold-water-fish probiotics bacillus strain and application thereof. The strain HZC58, which has anti-virus, immune adjustment and immunity enhancement characteristics for cold water fishes, is separated and identified from an intestinal tract mucous membrane of a juvenile semi-wild rainbow trout in Benxi mountainous area of Liaoning province. According to colony morphological characteristic observation, 16SrDNA base sequence determination and homology analysis, the identification result of the strain indicates that the HZC58 belongs to bacillus and the collection number is CGMCC No.10706. In-vitro cell experiments prove that the bacillus strain HZC58 is cultured together with EPCs (endothelial progenitor cells) and has the characteristic of resisting IHNV (infectious hematopoietic necrosis virus) in EPC proliferation in vitro. Animal experiments prove that the bacillus strain HZC58 has the immune adjustment and immunity enhancement characteristics for the cold water fishes, and the strain HZC58 has also the anti-virus characteristic for the cold water fishes when being used for feeding individually. The bacillus strain HZC58 has a certain application prospect in cold-water-fish anti-virus, immunity adjustment and immunity enhancement.

Description

technical field [0001] The invention relates to a probiotic bacterial strain and its application, in particular to a cold-water fish (rainbow trout) probiotic Bacillus HZC58 bacterial strain and its application. The invention belongs to the technical field of microorganisms. Background technique [0002] Probiotics are the normal flora of human and animal intestinal tract, respiratory tract, reproductive tract and skin mucous membrane system. Many research results have found that lactic acid bacteria and intestinal mucosa together form a protective barrier to prevent the invasion of viruses and bacteria. Human beings are increasingly aware of In view of the powerful effect of probiotics on human and animal life and health, a large number of modern immunological research results have confirmed that probiotics (especially lactic acid bacteria) can regulate animal cellular immunity, humoral immunity and local immunity of intestinal mucosa, as well as immune system development an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/18A23K1/17A61K39/39A61K39/205A61K35/742A61K39/385A61P31/14A61P37/02A61P37/04C12R1/07
CPCY02A40/818
Inventor 王云峰崔红玉王笑梅
Owner 哈尔滨国生生物科技股份有限公司
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