Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Copper ion detection reagent and application

A copper ion detection and copper ion technology, applied in the field of chemical detection, can solve the problems of instrument detection interference, high detection cost, difficult to popularize and apply, etc., and achieve the effects of fast response, simple preparation process and uniform particle size

Inactive Publication Date: 2015-12-09
XUZHOU NORMAL UNIVERSITY
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional instrument detection such as AAS and ICP-AES undoubtedly have good sensitivity, but due to the dependence on expensive detection instruments, the detection cost is high, and it is difficult to popularize and apply
In addition, the detection of these instruments is extremely susceptible to interference from the sample background (impurities, plankton, metal ions, etc.)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Copper ion detection reagent and application
  • Copper ion detection reagent and application
  • Copper ion detection reagent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a. 200μL 242mMHAuCl 4 Add 9100 μL of secondary water, and keep the temperature of the water bath at 65°C; quickly add an appropriate amount of freshly prepared PEI (Mw10000, 10mM) solution to the above solution, stir at a constant speed, and continue to react for 1h until the color becomes stable wine red, in which PEI and HAuCl 4 The ratio is 1:7; after the above solution is cooled to room temperature, centrifuge at 3000r / min for 15 minutes, repeat twice to remove larger particles, and obtain a stable copper ion detection reagent, that is, PEI-modified gold nanoparticles (PEI / AuNPs).

[0022] b. Take 80 μL of the gold nanoparticle solution prepared above and dissolve it in PBS buffer solution (pH 7.4).

[0023] c. Add Cu to the above system 2+ Standard solution, after incubating for 5 minutes, the reaction is fully carried out before detection, the detection principle is as follows: figure 1 Shown; Take above-mentioned nano-sol, test ultraviolet-visible spectrum.

...

Embodiment 2

[0029] The experimental process and experimental steps are the same as those in Example 1a-c. Cu 2+ The standard solution was replaced with water from Yuquan River of Jiangsu Normal University. Take 15, 62, 125, 220μM Cu respectively 2+ The Yuquan River water of the standard solution was tested, and the calculated standard addition recovery rate was 95%-101%, and the relative standard deviation was 2.2%.

Embodiment 3

[0031] The experimental process and experimental steps are the same as those in Example 1a-c. Cu 2+ The standard solution was replaced with wastewater from a chemical plant (Xuzhou, China). Take 500 μL after diluting 5 times of industrial wastewater for detection, and substitute it into the formula to calculate Cu 2+ The concentration was 162.87μM, which was very close to the detection result of atomic absorption method (168.32μM).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a copper ion detection reagent and application, and belongs to the technical field of chemical detection. The detection reagent is a polyethyleneimine modified water-soluble nano particle solution; the preparation method comprises the following steps: dissolving gold salt or silver salt in deionized water, adding polyethyleneimine of a certain proportion, and uniformly mixing to prepare the solution containing water-soluble nano particles with the surfaces being modified by amino groups; the detection method comprises the following steps: taking a proper amount of nano particles, adding the proper amount of nano particles into a phosphate buffer solution (PBS) of a certain pH value, adding a copper ion sample, then observing the color change of the solution and testing ultraviolet-visible absorption spectrum, and processing data. According to the copper ion detection reagent and application, provided by the invention, the preparation process of the reagent is simple, the reaction time is only 1 hour, and complicated treatment is not needed, therefore the reagent can be directly used; besides, as both the ultraviolet-visible spectrum analysis technique and the visual method are adopted, the color change of the solution is observed, the response to Cu<2+> is quick, the selectivity is high, high simplicity and convenience are achieved, and the copper ion detection reagent is applicable to rapid field detection of Cu<2+> in environmental water.

Description

technical field [0001] The invention relates to a copper ion detection reagent and its application, belonging to the technical field of chemical detection. Background technique [0002] With the rapid development of industry, heavy metal pollution has become increasingly prominent, Cu 2+ As one of the most common toxic heavy metals, it has attracted widespread attention because of its serious harm to animals, plants and the environment. Cu 2+ Cu is one of the essential trace elements for the human body. Many proteins and enzymes contain Cu 2+ , Cu 2+ It is closely related to cell reproduction, various enzyme activities, endocrine function and hematopoietic function, but if the body contains excessive Cu for a long time 2+ If it cannot be degraded, it will cause harm to the human body. Cu 2+ Pollution also threatens the safety of aquatic organisms. According to reports, copper content in water reaching 0.002mg / L will cause toxicity to fish. Cu 2+ The water pollution c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 盖宏伟赵文峰
Owner XUZHOU NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com