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Preparation method of hemoglobin electrochemical sensor

A hemoglobin and electrochemical technology, applied in the field of electrochemical sensor preparation, can solve the problems of biological fouling on the electrode surface, and achieve good practical application value, accurate results, and wide detection range

Active Publication Date: 2015-12-16
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, both metal nanoparticles and carbon nanotubes will exhibit non-specific adsorption behavior, resulting in biofouling on the electrode surface.

Method used

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  • Preparation method of hemoglobin electrochemical sensor
  • Preparation method of hemoglobin electrochemical sensor
  • Preparation method of hemoglobin electrochemical sensor

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of silica nanomaterials: Mix 22.5mL cyclohexane, 4.8mL n-hexanol and 5.778g TritonX-100 (polyethylene glycol octylphenyl ether, detergent or non-ionic surfactant), stir After about 5 minutes to obtain a clear solution, 1.44 mL of ultrapure water was added as the dispersed phase, and the stirring was continued for 30 minutes until a transparent and stable water-in-oil microemulsion was formed. Add 300 μL NH 3 ·H 2 O and 600μLTEOS (tetraethyl orthosilicate) were added to the above-mentioned water-in-oil microemulsion, and stirred continuously to make it react for 24h. Then use absolute ethanol with a volume ratio of 1:1 to break the emulsion, and centrifuge to obtain white silica nanomaterials.

[0031] See the transmission electron microscopy data of white silica nanomaterials in figure 1 ,From figure 1 It can be seen that the silica nanomaterials are spheres with smooth surface and uniform particle size.

[0032]2. Preparation of silica-gold nanocomp...

Embodiment 2

[0034] Preparation of silica-gold nanocomposites: Mix 20mL of isopropanol and 400μLAPTES (3-aminopropylethoxysilane), add 30mg of the prepared silica nanomaterials, stir continuously at 80°C for 2h, then Add isopropanol and centrifuge twice to get the solid phase, then add ultrapure water and centrifuge once to get the solid phase. The resulting solid-phase product was added to a mixture of 20 mL trisodium citrate (2.5 × 10 -4 mol / L) and HAuCl 4 (0.1×10 -3 mol / L), stirred at room temperature for 1 h, and then added 600 μL of NaBH with a concentration of 0.1 mol / L 4 After stirring the aqueous solution for 1 hour, adding absolute ethanol and centrifuging for 3 times, taking the solid phase, adding ultrapure water and centrifuging for 3 times, the silica-gold nanocomposite material can be obtained. The transmission electron microscopy data of silica-gold nanocomposites are shown in image 3 ,From image 3 It can be seen in: Au is connected to the surface of spherical silica ...

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Abstract

The invention discloses a preparation method of a hemoglobin electrochemical sensor. The method comprises steps as follows: a silicon dioxide nanomaterial is prepared firstly, then a silicon dioxide-gold nanoparticle composite material with good biocompatibility is prepared and modified on the surface of an electrode, so that the electrochemical sensor capable of directly detecting the concentration of hemoglobin in precooling water is prepared, and the application of the nanoparticle composite material in the aspect of biochemical analysis and a biosensor precooling water detection technology are realized. With the adoption of the method, the influence of common interference substances in the precooling water is avoided, the method has the advantages that the detection range is wide, the reproducibility is good, the result is accurate and the like, meanwhile, precooling water detection avoids treatment of detection samples and longer analysis time, and the actual application value is very good.

Description

technical field [0001] The invention relates to an electrochemical sensor, in particular to a preparation method of the electrochemical sensor for detecting the concentration of hemoglobin in poultry slaughtering pre-cooling water. technical background [0002] Hemoglobin (Hb) is a protein responsible for carrying oxygen in higher organisms. It is very important in life and is often used as a tracer of biomolecules. Hb can not only transport oxygen in the living body, but also decompose H 2 o 2 , transfer electrons, energy metabolism and other important roles. The traditional detection methods of hemoglobin are mainly spectroscopic method, fluorescence method and colorimetric method. Spectrometry and fluorescence methods have cumbersome sample processing processes, and it takes a long time from sampling to detection, and professional detection equipment requires specialized operators, which greatly limits the possibility of widespread use; while colorimetry is through the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 孙冲徐宝才刘丽华徐为民王道营李聪
Owner JIANGSU ACAD OF AGRI SCI