A kind of preparation method of hemoglobin electrochemical sensor
A hemoglobin and electrochemical technology, applied in the field of electrochemical sensor preparation, can solve problems such as biofouling on the surface of electrodes, and achieve the effects of good practical application value, accurate results and wide detection range
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Embodiment 1
[0030] 1. Preparation of silica nanomaterials: Mix 22.5mL cyclohexane, 4.8mL n-hexanol and 5.778g Triton X-100 (polyethylene glycol octylphenyl ether, detergent or non-ionic surfactant) , stirred for about 5 min to obtain a clear solution, added 1.44 mL of ultrapure water as the dispersed phase, and continued to stir for 30 min until a transparent and stable water-in-oil microemulsion was formed. Add 300 μL NH 3 ·H 2 O and 600 μL TEOS (tetraethyl orthosilicate) were added to the above water-in-oil microemulsion, and stirred continuously to make it react for 24 h. Then use absolute ethanol with a volume ratio of 1:1 to break the emulsion, and centrifuge to obtain white silica nanomaterials.
[0031] See the transmission electron microscopy data of white silica nanomaterials in figure 1 ,from figure 1 It can be seen that the silica nanomaterials are spheres with smooth surface and uniform particle size.
[0032]2. Preparation of silica-gold nanocomposites: Mix 20mL of isopr...
Embodiment 2
[0034] Preparation of silica-gold nanocomposites: Mix 20 mL of isopropanol and 400 μL of APTES (3-aminopropylethoxysilane), add 30 mg of the prepared silica nanomaterials, and continuously stir at 80 °C for 2 h , and then add isopropanol and centrifuge twice to get the solid phase, then add ultrapure water and centrifuge once to get the solid phase. The resulting solid-phase product was added to a mixture of 20 mL trisodium citrate (2.5 × 10 -4 mol / L) and HAuCl 4 (0.1×10 -3 mol / L), stirred at room temperature for 1 h, and then added 600 μL of NaBH with a concentration of 0.1 mol / L 4 After stirring the aqueous solution for 1 h, add absolute ethanol and centrifuge 3 times, take the solid phase, add ultrapure water and centrifuge 3 times, and the silica-gold nanocomposite material can be obtained. The transmission electron microscopy data of silica-gold nanocomposites are shown in image 3 ,from image 3 It can be seen in: Au is connected to the surface of spherical silica p...
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