A skin care composition for whitening and lightening spots
A skin care composition and composition technology, which is applied in the field of whitening and lightening cosmetic compositions, and can solve problems such as troxerutin not seen
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experiment example 1
[0085] Measure the effect of inhibiting melanin formation
[0086] The HM3KO cells, which are human melanoma cells, were placed in a minimal essential medium containing 10% bovine embryonic serum and cultured at 37°C and 5% CO2. Smear the cultured cells on the bottom of a 75-well flask with a cell count of 3×105 / well and leave it overnight to make the cells adhere to the wall. After confirmation, each medium was changed to a new medium containing 10 ppm experimental sample.
[0087] The experimental samples used are shown in Table 1 below.
[0088] The control group used a medium containing DMSO (dimethyl sulfoxide).
[0089] In the above manner, each medium was changed to a new medium containing the experimental sample every 2 days until the cultured cells were enough to fill the flask. Then, the medium fluid was taken out, washed with PBS (phosphate buffered saline), and re-dissolved in 1N NaOH. The optical density (OD) at 500 nm was measured, and the inhibition rate of melanin f...
experiment example 2
[0095] Skin whitening effect on human skin
[0096] An opaque tape with 6 holes with a diameter of 1.5 cm was adhered to the upper arms of each of 10 healthy men, and irradiated with an ultraviolet beam with an intensity of 1.5-2 times the minimum erythema dose of each subject. Induce skin melanin deposition. Then apply the test sample, after 2 months, use the colorimeter to measure the depth of the skin.
[0097] The experimental samples were prepared according to the composition in Table 1, and the preparations of the examples in Table 1 and the comparative preparations were applied twice a day (once in the morning and once in the evening).
[0098] table 3
[0099] Matrix formula
[0100]
[0101] Table 3 Active ingredient ratio
[0102]
[0103] The experimental results are shown in the table below
[0104] Table 4
[0105] Experimental sample
Skin whitening effect (ΔL)
Formulation 1
1.02
Formulation 2
1.21
Formulation 3
0.72
Formulation 4
3.72
Comparative preparation...
Embodiment 1
[0109] Butylene glycol 6.0
[0110] Methyl paraben 0.12
[0111] Hydroxyethyl cellulose 0.2
[0112] Polyquaternium-100.05
[0113] Troxerutin 1.5
[0114] EDTA disodium 0.15
[0115] Allantoin 0.15
[0116] Serine 2.0
[0117] Trimethylglycine 2.5
[0118] Carbomer 0.4
[0119] Caprylic / Capric Triglyceride 6.35
[0120] Cetearyl Glucoside 0.75
[0121] Cetearyl Alcohol 2.0
[0122] PEG-100 stearate 1.0
[0123] Stearic acid monoglyceride 1.0
[0124] PEG-75 Lanolin 0.2
[0125] Polydimethylsiloxane 2.0
[0126] Bisabolol 0.35
[0127] Tocopherol Acetate 2.0
[0128] Sapindus saponins 2.0
[0129] Ginsenoside 0.4
[0130] Nicotinamide 4.0
[0131] Anti-allergic agent 2.0 (Korea Youai Company sells under the trade name U-activeP8)
[0132] Phenoxyethanol 0.5
[0133] Flavor 0.35
[0134] Glycerin 4.0
[0135] Urea 0.2
[0136] Papain 3.0
[0137] Water to 100.
PUM
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