Biosensor based on metal organic skeleton material and application thereof

A metal-organic framework and biosensor technology, which is applied in the field of biosensors to achieve the effects of rapid detection response, improved sensitivity and high selectivity

Inactive Publication Date: 2015-12-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no precedent for the detection of bisphenols compounds based on tyrosinase electrochem

Method used

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  • Biosensor based on metal organic skeleton material and application thereof
  • Biosensor based on metal organic skeleton material and application thereof
  • Biosensor based on metal organic skeleton material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Preparation of tyrosinase electrochemical biosensor

[0046] Glassy carbon electrode assembly steps:

[0047] (1) Polish the surface of the glassy carbon electrode with aluminum oxide powders with particle sizes of 1 μm, 0.3 μm, and 0.05 μm, and then repeatedly ultrasonically clean it in anhydrous ethanol and deionized water, and then dry the electrode surface with nitrogen gas. use. Put in 1mmolL -1 Potassium ferricyanide / potassium ferrocyanide solution (molar ratio 1:1) at -0.1 ～ Scan the cyclic voltammetry curve between +0.6V. The redox peak potential difference of the curve is less than 70mV, indicating that the redox reaction on the surface of the glassy carbon electrode is a completely reversible reaction, and the electrode polishing is in good condition, and the next experiment can be carried out.

[0048] (2) Preparation of metal organic framework material-tyrosinase composite material. Metal-organic framework material was added to tyrosinase pho...

Embodiment 2

[0051] Example 2. Electrochemical Characterization of Tyrosinase Electrochemical Biosensors

[0052] On the PGSTAT302N electrochemical workstation, the CuMOF-Tyr-Chi / GCE, CuMOF-Chi / GCE, Chi / GCE and bare GCE prepared in the above example 1 were respectively measured in the presence of 1 mmolL- 1 Potassium ferricyanide / potassium ferrocyanide (molar ratio 1:1) probe and 0.5molL- 1 AC impedance signal in electrolyte with potassium nitrate supported electrolyte. Experimental results such as figure 2 As shown, the order of charge transfer resistance of different modified electrodes is: CuMOF-Tyr-Chi / GCE>CuMOF-Chi / GCE>Chi / GCE>bareGCE, indicating that the CuMOF-Tyr-Chi composite material in the present invention has been successfully modified in Electrode surface, successfully prepared tyrosinase electrochemical biosensor.

Embodiment 3

[0053] Example 3. The standard solution for detection of bisphenol compounds by tyrosinase electrochemical biosensor

[0054] Add a certain volume (0.4-2μL) of bisphenol A standard solution (1mmol / L) continuously dropwise (once every 50s) to the detection solution of the three-electrode system composed of tyrosinase-modified glassy carbon electrodes, 8ml of phosphate buffered saline In the solution, under the situation that working potential is-0.1V, carry out potentiostatic scanning, the current-time (I-t) relationship curve that obtains, because the bisphenol A concentration in the detection solution in the fixed time point in I-t curve is known, so can obtain Correlation curve between current intensity and bisphenol A concentration. In the presence of oxygen, tyrosinase catalyzes bisphenol A to generate o-phenolic compounds, which are further catalyzed to generate o-quinone compounds, which are then reduced to o-phenolic compounds at a lower potential. The tyrosinase elect...

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Abstract

The invention discloses a tyrosinase electrochemical biosensor based on a metal organic skeleton material and chitosan and a preparation method and application thereof. The preparation method includes the steps that tyrosinase is first fixedly carried on the surface of the metal organic skeleton material (CuMOF) with copper ions being a metal center and then mixed with chitosan (Chi) to generate a CuMOF-Tyr-Chi composite material so that the composite material can be fixed to the surface of a glassy carbon electrode to prepare the tyrosinase electrochemical biosensor. The biosensor has the advantages that sensitivity for a bisphenol type compound is high; the detection limit is low; selectivity is good; the biosensor is easy to prepare without a complex sample pre-treatment process, response to a target compound is rapid, and miniaturization is easy.

Description

technical field [0001] The invention belongs to biosensors, and relates to a method for detecting bisphenol compounds by a tyrosinase electrochemical biosensor based on a metal organic framework material-chitosan composite material. Background technique [0002] Bisphenol A, or 2,2-bis(4-hydroxyphenyl)propane (BPA for short), is an endocrine disruptor that has received widespread attention in recent years. Bisphenol A interacts with human estrogen receptors to produce estrogenic effects and cause harm to human health. In addition, experiments have proved that bisphenol A has certain toxicity to the human central nervous system, immune system and reproductive system. Bisphenol A is one of the most widely used industrial raw materials in the world, mainly used to produce polycarbonate and epoxy resin. These plastics are often used to produce baby bottles, cutlery, drinking cups and coatings on the inside of beverage cans. Canada, the United States, China and other countries...

Claims

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Application Information

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IPC IPC(8): G01N27/416
Inventor 王雪卢宪波陈吉平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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