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Method for utilizing dextran fermentation waste liquor for succinic acid fermentation production

A technology of fermenting waste liquid and dextran, which is applied in the field of bioengineering, can solve the problems of large waste of resources and difficult waste liquid treatment, and achieve the effects of reducing waste water treatment costs, reducing waste water discharge, and good economic and environmental benefits

Active Publication Date: 2016-01-06
陈栋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is: according to domestic dextran manufacturers waste liquid treatment is difficult, the waste of resources is big problem, provide a kind of method of effective waste liquid comprehensive utilization, this method has no effect on the production level of succinic acid and product quality Obvious adverse effects, less process steps, good economic and environmental benefits

Method used

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  • Method for utilizing dextran fermentation waste liquor for succinic acid fermentation production
  • Method for utilizing dextran fermentation waste liquor for succinic acid fermentation production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Batch fermentation of succinic acid with dextran fermentation waste liquid as carbon source

[0021] a) Preparation of fermentation medium

[0022] Take 1.45L of dextran fermentation waste liquid (reducing sugar content detected as 61.5g / L) after about three times of concentration as the mother liquor, add other ingredients in the fermentation medium to prepare the fermentation medium, corn steep liquor 25g / L, K 2 HPO 4 1.5g / L, NaH 2 PO 4 2.2g / L, sodium glutamate 2g / L, biotin 0.01g / L, Na 2 S0.01g / L, adjust the pH to 6.2, and sterilize at 121°C for 0min.

[0023] b) Seed cultivation

[0024] Actinobacillus succinicum CCTCCNO: M2012036 The first-class seeds of the shake flask were cultured statically for 12 hours in an anaerobic incubator, and the cultivation temperature was 38°C. The first-class seeds were inserted into 150mL second-class seeds with an inoculum amount of 2.5% (volume fraction) (The composition of the seed medium is: glucose 10g / L, yeast ...

Embodiment 2

[0029]Example 2: Batch fermentation of succinic acid with dextran fermentation waste liquid as carbon source

[0030] a) Preparation of fermentation medium

[0031] Adjust the dextran fermentation waste liquid to the reducing sugar content of about 90g / L, and add corn steep liquor 35g / L, K 2 HPO 4 3g / L, NaH 2 PO 4 3g / L, sodium glutamate 0.05g / L, biotin 0.5g / L, Na 2 S0.5g / L, adjust the pH to 6.0, and make it into a fermentation medium.

[0032] b) Seed cultivation

[0033] The seed solution of Actinobacillus succinate CCTCC NO: M2012036 was prepared.

[0034] c) Succinic acid fermentation

[0035] The initial reducing sugar concentration of the fermentation medium is about 90g / L, the temperature is 37°C, the fermentation time is 50h, and the rotation speed is 150r / min. 2 CO 3 Control pH6.0-6.5, finish fermentation when the concentration of succinic acid in the fermentation broth no longer increases, the result shows that the output of succinic acid can reach 48.2g / L. ...

Embodiment 3

[0036] Embodiment 3: using dextran fermentation waste liquid as carbon source fed batch fermentation to produce succinic acid

[0037] a) Fermentation medium and feed preparation

[0038] Take 1.6L tap water, add other ingredients in the fermentation medium to prepare the fermentation medium, corn steep liquor 25g / L, K 2 HPO 4 2g / L, NaH 2 PO 4 2.2g / L, sodium glutamate 2g / L, biotin 0.01g / L, Na 2 S0.01g / L, glucose 30g / L, adjust the pH to 6.3, and sterilize at 121°C for 20min.

[0039] After concentrating the dextran fermentation waste liquid by about 10 times its volume (reducing sugar content in it was detected to be 305.4g / L), take 0.6L into a feeding bottle, sterilize at 115°C for 20min, and connect the feeding bottle to the fermenter after sterilization superior.

[0040] b) seed culture as described in Example 1

[0041] c) Succinic acid fermentation

[0042] The initial glucose concentration of the fermentation medium was 30g / L, the temperature was 37°C, the fermen...

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Abstract

The invention discloses a method for utilizing dextran fermentation waste liquor for succinic acid fermentation production, and belongs to the technical field of biological engineering. According to the method, the dextran fermentation waste liquor serves as mother liquor to prepare a succinic acid fermentation culture medium, and 30-40 g / L of succinic acid can be produced through batch fermentation; or the mode of taking 10-40 g / L glucose as a carbon source of an initial fermentation culture medium, and concentrated dextran fermentation waste liquor which is subsequently added is adopted for material supplementary to produce succinic acid through batch fermentation, the concentration of succinic acid can reach 40-55.0 g / L after 40-60 hours, the production intensity ranges from 0.8 g / L / h to 1.0 g / L / h, and the sugar acid conversion ratio ranges from 0.70 g / g to 0.83 g / g. According to the method for utilizing the dextran fermentation waste liquor for succinic acid fermentation production, reducing sugar in the dextran fermentation waste liquor can be fully utilized to serve as the carbon source for producing the succinic acid through fermentation, the wastewater discharge amount in the dextran production process is effectively reduced, the use amount of glucose for preparing the succinic acid fermentation culture medium is decreased, and waste utilization of the dextran fermentation waste liquor is achieved.

Description

technical field [0001] The invention discloses a method for using dextran fermentation waste liquid in succinic acid fermentation production, belonging to the technical field of bioengineering. Background technique [0002] Dextran is a high-molecular glucose polymer. It is the first to be used and recognized as an excellent plasma substitute. It is widely used in medicine, food and petroleum. Dextran is also the world's first industrially produced microbial polysaccharide. High-concentration sucrose is fermented by Leuconostoc enterococcus to produce high-molecular glucose polymers, which are then refined to obtain dextran products with different molecular weights. After the products are extracted, the remaining fermentation broth It is dextran fermentation waste liquid. Dextran fermentation waste liquid is generally used as industrial wastewater for environmental protection treatment, but it will add additional environmental protection treatment costs to enterprises. Sin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C07C55/10
Inventor 郑璞陈鹏程查鑫华
Owner 陈栋
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