Method for extracting aucubin and geniposide from eucommia ulmoides peel at same time

Abstract

The invention discloses a method for extracting aucubin and geniposide from eucommia ulmoides peel at the same time. The method includes the steps of firstly, weighing aucubin, geniposide and cobaltous acetate to be dissolved in an acetonitrile-dimethyl sulfoxide mixed solution according to a certain proportion, conducting shaking, adding functional monomers to be shaken, adding mixed cross-linking agent TRIM-DVB and initiator AIBN to be sufficiently mixed, transferring the mixture into a round-bottom flask, and conducting degassing, argon introducing and bottle sealing; secondly, putting the mixture in a constant-temperature water bath kettle at the temperature of 60 DEG C to be subjected to a polymerization reaction, and conducting smashing, screening, depositing, filtering, eluting and vacuum drying; thirdly, putting obtained molecularly imprinted polymers into a solid-phase extraction cylinder, conducting washing and eluting after sample adding, and obtaining aucubin and geniposide. Through the metal ion coordination imprinting technology, binary compound metal ion complex serves as a template molecule, molecule imprinting polymers with high selection recognition performance is synthesized, and quite high practical value is achieved when aucubin and geniposide are extracted from eucommia ulmoides peel at the same time through the polymers.

Description

technical field [0001] The invention relates to a method for simultaneously extracting aucubin and geniposide from eucommia bark by using a newly prepared molecular imprinted material. Background technique [0002] Molecularly imprinted technology aims to construct supramolecular compounds with high selectivity and even specific binding ability to target compounds. Due to the matching of the binding site with the target molecule in terms of shape, size and chemical functional groups, molecularly imprinted polymers can be used It is used for selective enrichment and even specific identification of target compounds in complex systems. So far, molecular imprinting technology has been widely used in solid phase extraction, chromatographic separation, sensing technology, immunoassay and simulated enzyme catalysis and other fields. However, most studies on molecular imprinting in the past mainly focused on the imprinting preparation and molecular recognition of a single template....

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects of low selectivity, low recovery rate, and low product purity in the existing complex sample extraction technology, and provide a method for molecularly imprinted solid-phase extraction of the target substance, through metal ions, multiple Template molecules, functional monomers are effectively coordinated and then imprinted to obtain a metal Co 2+ -Binary iridoid glycoside coordination imprinting material, and use it as solid phase extraction material to simultaneously extract aucubin and geniposide in Eucommia