Method for extracting aucubin and geniposide from eucommia ulmoides peel at same time

A technology of aucubin and geniposide, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low recovery rate, low product purity, and low selectivity, and achieve Improve the realism, improve the bonding strength, and strengthen the effect of practical value

Inactive Publication Date: 2016-01-20
河北汇正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects of low selectivity, low recovery rate, and low product purity in the existing complex sample extraction technology, and provide a method for molecularly imprinted solid-phase extraction of the target substance, t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of molecularly imprinted materials: Weigh the template molecule aucubin-geniposide mixture and cobalt acetate dissolved in acetonitrile-dimethyl sulfoxide mixed solution and shake for 10 minutes, add functional monomer 4-VP, shake After 30 minutes, let it fully react with the functional monomer, then add the mixed crosslinking agent TRIM-DVB (molar ratio 1:1) and the initiator AIBN, after fully mixing, transfer to a round bottom flask, ultrasonically degas for 15 minutes, and then pass in argon Gas for 15 minutes, seal the bottle; put the round-bottomed flask into a 60°C constant temperature water bath for 24 hours of polymerization reaction, crush the obtained massive polymer, pass through a 200-mesh sieve, settle with acetone for 3 times, each time for 30 minutes, and discard the upper layer The turbid liquid is used to remove the fine particles in the polymer. After vacuum-drying the lower precipitate, wash away the metal ions in the polymer with 0.10m...

Embodiment 2

[0018] (1) Preparation of molecularly imprinted materials: Weigh the template molecule aucubin-geniposide mixture and cobalt acetate dissolved in acetonitrile-dimethyl sulfoxide mixed solution and shake for 10 minutes, add functional monomer 4-VP, shake After 30 minutes, let it fully react with the functional monomer, then add the mixed crosslinking agent TRIM-DVB (molar ratio 1:1) and the initiator AIBN, after fully mixing, transfer to a round bottom flask, ultrasonically degas for 15 minutes, and then pass in argon Gas for 15 minutes, seal the bottle; put the round-bottomed flask into a 60°C constant temperature water bath for 24 hours of polymerization reaction, crush the obtained massive polymer, pass through a 200-mesh sieve, settle with acetone for 3 times, each time for 30 minutes, and discard the upper layer The turbid liquid is used to remove the fine particles in the polymer. After vacuum-drying the lower precipitate, wash away the metal ions in the polymer with 0.10m...

Embodiment 3

[0022] (1) Preparation of molecularly imprinted materials: Weigh the template molecule aucubin-geniposide mixture and cobalt acetate dissolved in acetonitrile-dimethyl sulfoxide mixed solution and shake for 10 minutes, add functional monomer 4-VP, shake After 30 minutes, let it fully react with the functional monomer, then add the mixed crosslinking agent TRIM-DVB (molar ratio 1:1) and the initiator AIBN, after fully mixing, transfer to a round bottom flask, ultrasonically degas for 15 minutes, and then pass in argon Gas for 15 minutes, seal the bottle; put the round-bottomed flask into a 60°C constant temperature water bath for 24 hours of polymerization reaction, crush the obtained massive polymer, pass through a 200-mesh sieve, settle with acetone for 3 times, each time for 30 minutes, and discard the upper layer The turbid liquid is used to remove the fine particles in the polymer. After vacuum-drying the lower precipitate, wash away the metal ions in the polymer with 0.10m...

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PUM

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Abstract

The invention discloses a method for extracting aucubin and geniposide from eucommia ulmoides peel at the same time. The method includes the steps of firstly, weighing aucubin, geniposide and cobaltous acetate to be dissolved in an acetonitrile-dimethyl sulfoxide mixed solution according to a certain proportion, conducting shaking, adding functional monomers to be shaken, adding mixed cross-linking agent TRIM-DVB and initiator AIBN to be sufficiently mixed, transferring the mixture into a round-bottom flask, and conducting degassing, argon introducing and bottle sealing; secondly, putting the mixture in a constant-temperature water bath kettle at the temperature of 60 DEG C to be subjected to a polymerization reaction, and conducting smashing, screening, depositing, filtering, eluting and vacuum drying; thirdly, putting obtained molecularly imprinted polymers into a solid-phase extraction cylinder, conducting washing and eluting after sample adding, and obtaining aucubin and geniposide. Through the metal ion coordination imprinting technology, binary compound metal ion complex serves as a template molecule, molecule imprinting polymers with high selection recognition performance is synthesized, and quite high practical value is achieved when aucubin and geniposide are extracted from eucommia ulmoides peel at the same time through the polymers.

Description

technical field [0001] The invention relates to a method for simultaneously extracting aucubin and geniposide from eucommia bark by using a newly prepared molecular imprinted material. Background technique [0002] Molecularly imprinted technology aims to construct supramolecular compounds with high selectivity and even specific binding ability to target compounds. Due to the matching of the binding site with the target molecule in terms of shape, size and chemical functional groups, molecularly imprinted polymers can be used It is used for selective enrichment and even specific identification of target compounds in complex systems. So far, molecular imprinting technology has been widely used in solid phase extraction, chromatographic separation, sensing technology, immunoassay and simulated enzyme catalysis and other fields. However, most studies on molecular imprinting in the past mainly focused on the imprinting preparation and molecular recognition of a single template....

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 李辉王素素张月龚梦婷
Owner 河北汇正生物科技有限公司
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