Mold-culture-containing enzyme special for growing pigs and preparation method thereof
A technology for cultivating and growing pigs with mold, which is applied in the field of special enzymes for growing pigs containing mold cultures and its preparation, which can solve the problems of culture functions and preparation methods that need to be improved, the activity of enzyme preparations that need to be continuously improved, and the special enzyme enzyme system is incomplete, etc. problems, to achieve the effect of regulating the balance of gastrointestinal flora, inhibiting growth and reproduction, and promoting animal growth
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Embodiment 1
[0060] Embodiment 1 raw material preparation
[0061] Preparation of mold cultures:
[0062] The preparation method of described mold culture comprises the following steps:
[0063] (1) Activation of bacteria
[0064] Inoculate the well-preserved slant strain of Aspergillus usami CCTCCNO: M2013601 on the slant medium, culture at 32°C for 40 hours to activate the strain, and activate it 3 times;
[0065] (2) Expanded cultivation of liquid seeds
[0066] ① First-level seed cultivation: Wash the spores of the slanted bacteria after activation in step (1) with sterile water, insert them into a 500-ml shaker flask, fill the liquid seed medium with 100 ml, and culture on a shaker at 32°C and 100 rpm for 40 hours;
[0067] ②Cultivation of secondary seeds: Inoculate 10% of the primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as those of the primary seeds;
[0068] ③Third-level seed cultivation: insert the second-level seeds into a ...
Embodiment 2
[0085] A special enzyme for growing pigs containing mold culture, which consists of the following raw materials in parts by weight:
[0086] 50 parts of mold culture, 40 parts of plant extract, 35 parts of acid xylanase, 25 parts of cellulase, 25 parts of beta-glucanase, 18 parts of pectinase, 18 parts of amylase, 12 parts of mannose 12 parts of phytase, 8 parts of Chinese herbal medicine extract, 7 parts of protective agent, 7 parts of activator, 4 parts of antioxidant;
[0087] The mold culture, plant extract, and Chinese herbal medicine extract are all prepared in Example 1;
[0088] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;
[0089] Described protecting agent is made up of the raw material of following parts by weight: trehalose 25 parts, NaCl25 parts, (NH 4 ) 2 SO 4 12 parts, cysteine 12 parts;
[0090] The activator is formed by uniformly mixing inorganic salts with the following mass ...
Embodiment 3
[0095] A special enzyme for growing pigs containing mold culture, which consists of the following raw materials in parts by weight:
[0096] 40 parts of mold culture, 30 parts of plant extract, 30 parts of acid xylanase, 20 parts of cellulase, 20 parts of β-glucanase, 15 parts of pectinase, 15 parts of amylase, 10 parts of mannose 10 parts of phytase, 6 parts of Chinese herbal medicine extract, 5 parts of protective agent, 5 parts of activator, 3 parts of antioxidant;
[0097] The mold culture, plant extract, and Chinese herbal medicine extract are all prepared in Example 1;
[0098] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;
[0099] Described protecting agent is made up of the raw material of following parts by weight: 20 parts of trehalose, 20 parts of NaCl, (NH 4 ) 2 SO 4 10 parts, cysteine 10 parts;
[0100] The activator is formed by uniformly mixing inorganic salts with the following...
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