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Mold-culture-containing enzyme special for growing pigs and preparation method thereof

A technology for cultivating and growing pigs with mold, which is applied in the field of special enzymes for growing pigs containing mold cultures and its preparation, which can solve the problems of culture functions and preparation methods that need to be improved, the activity of enzyme preparations that need to be continuously improved, and the special enzyme enzyme system is incomplete, etc. problems, to achieve the effect of regulating the balance of gastrointestinal flora, inhibiting growth and reproduction, and promoting animal growth

Active Publication Date: 2016-02-10
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Chinese patent CN103865904A discloses a special enzyme for growing pigs containing probiotics and a preparation method thereof. The special enzyme for growing pigs contains a culture of Bacillus licheniformis; Enzymes and preparation methods thereof, the special enzyme for growing pigs contains Aspergillus niger culture; the above-mentioned published patent scientifically compounded microbial cultures containing different types of enzyme preparations on the basis of feed compound enzymes, which can improve the growth of pigs. Immune function and production performance have a certain effect, but the activity of the enzyme preparation in the culture needs to be continuously improved, the function and preparation method of the culture need to be improved, the special enzyme enzyme system is incomplete (excluding plant enzymes), and the special enzyme components have no freedom to add and eliminate Antioxidant that bases and enhances enzyme activity
There are certain defects in the special enzymes for growing pigs

Method used

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  • Mold-culture-containing enzyme special for growing pigs and preparation method thereof
  • Mold-culture-containing enzyme special for growing pigs and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0060] Embodiment 1 raw material preparation

[0061] Preparation of mold cultures:

[0062] The preparation method of described mold culture comprises the following steps:

[0063] (1) Activation of bacteria

[0064] Inoculate the well-preserved slant strain of Aspergillus usami CCTCCNO: M2013601 on the slant medium, culture at 32°C for 40 hours to activate the strain, and activate it 3 times;

[0065] (2) Expanded cultivation of liquid seeds

[0066] ① First-level seed cultivation: Wash the spores of the slanted bacteria after activation in step (1) with sterile water, insert them into a 500-ml shaker flask, fill the liquid seed medium with 100 ml, and culture on a shaker at 32°C and 100 rpm for 40 hours;

[0067] ②Cultivation of secondary seeds: Inoculate 10% of the primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as those of the primary seeds;

[0068] ③Third-level seed cultivation: insert the second-level seeds into a ...

Embodiment 2

[0085] A special enzyme for growing pigs containing mold culture, which consists of the following raw materials in parts by weight:

[0086] 50 parts of mold culture, 40 parts of plant extract, 35 parts of acid xylanase, 25 parts of cellulase, 25 parts of beta-glucanase, 18 parts of pectinase, 18 parts of amylase, 12 parts of mannose 12 parts of phytase, 8 parts of Chinese herbal medicine extract, 7 parts of protective agent, 7 parts of activator, 4 parts of antioxidant;

[0087] The mold culture, plant extract, and Chinese herbal medicine extract are all prepared in Example 1;

[0088] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;

[0089] Described protecting agent is made up of the raw material of following parts by weight: trehalose 25 parts, NaCl25 parts, (NH 4 ) 2 SO 4 12 parts, cysteine ​​12 parts;

[0090] The activator is formed by uniformly mixing inorganic salts with the following mass ...

Embodiment 3

[0095] A special enzyme for growing pigs containing mold culture, which consists of the following raw materials in parts by weight:

[0096] 40 parts of mold culture, 30 parts of plant extract, 30 parts of acid xylanase, 20 parts of cellulase, 20 parts of β-glucanase, 15 parts of pectinase, 15 parts of amylase, 10 parts of mannose 10 parts of phytase, 6 parts of Chinese herbal medicine extract, 5 parts of protective agent, 5 parts of activator, 3 parts of antioxidant;

[0097] The mold culture, plant extract, and Chinese herbal medicine extract are all prepared in Example 1;

[0098] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;

[0099] Described protecting agent is made up of the raw material of following parts by weight: 20 parts of trehalose, 20 parts of NaCl, (NH 4 ) 2 SO 4 10 parts, cysteine ​​10 parts;

[0100] The activator is formed by uniformly mixing inorganic salts with the following...

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Abstract

The invention discloses mold-culture-containing enzyme special for growing pigs and a preparation method thereof and belongs to the technical field of preparation of enzyme preparations. Barley malt, burnt malt, figs and pineapples which are rich in a variety of enzyme systems and garlic, vanilla beans, illicium verum and fennel which can enhance animal appetite and resist to and inhibit bacteria serve as main raw materials, the low temperature wall breaking and extraction technologies are adopted, effective constituents in the raw materials are extracted to the greatest extent, natural plant extract is prepared, meanwhile, aspergillus usamii which can produce high-vitality acid protease serves as an original strain for producing a mold culture containing high-vitality acidic protease, Chinese herbal extract, protective agents, activating agents, anti-oxidant and other food-grade feed enzyme are compounded scientifically, by means of the obtained feed compound enzyme, safe and comprehensive digestive enzyme is provided for the growing pigs, in addition, pleasant fragrance can be brought to feed, appetite of the growing pigs is enhanced, the digestion and absorption functions of the stomachs and intestines of the growing pigs are effectively improved, and productivity of the growing pigs is raised.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparation, in particular to a special enzyme for growing pigs containing mold culture and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations to feed: 1. Degradation of anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, resulting in indigestion of animals and decreased production performance. 2. Improve the utilization rate of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or are present in certain structures that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus is present in the form of phytate phosphorus.). 3. Degrade some specific chemical bonds in raw materials. These chemical bonds cannot be degraded by the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165
Inventor 李洪兵李海清张锦杰朱永明胡永明向左东
Owner 湖南新鸿鹰生物工程有限公司
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