Pharmaceutical compositions containing the extracts of Ilex latifolia as an active ingredient
A technology of holly vulgaris and active ingredients is applied in the directions of drug combinations, medical preparations containing active ingredients, functions of food ingredients, etc., which can solve the problems of unproven treatment effects of degenerative brain diseases, etc., and achieve the effect of inhibiting memory damage.
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Embodiment 1
[0051] The manufacture of embodiment 1 large-leaf holly (Ilexlatifolia) extract
[0052] Production of ethanol extract
[0053] Big-leaf holly is chopped and used after purchased at the Chinese herbal medicine market in Gyeongdong, Seoul. After preparing 100 g of Ilex japonica, it was extracted three times in a reflux condenser within three hours using 3 L of 100% ethanol at room temperature. The above solution was filtered through Waterman No. 1 paper and then dried to finally harvest 18.7 g of ethanol extract. The finally harvested holly extract is put into dimethyl sulfoxide (dimethylsulfoxide; DMSO) and dissolved into The concentration is stored at -20°C. During the experiment, the experiment buffer solution was diluted so that the final concentration of DMSO was less than 0.1% before use.
[0054] Manufacture of water extract
[0055] Except that water was used as the extraction medium instead of 100% ethanol, the powder (yield 21.5%) was obtained according to th...
Embodiment 2
[0060] Example 2 Induced cerebral infarction in rats, cerebral infarction and edema of Ilex japonicus extract Confirmation of inhibitory effect
[0061]The inventors of the present invention conducted the following experiment in order to confirm the efficacy of the extract of Ilex japonicus on rats with cerebral infarction. First, 300±5 g SD rats (SAMTAKO, Gyeonggi-do) were anesthetized by inhalation of 1-2% isoflurane (N2:O2=4:1), and the right carotid artery was carefully exposed surgically. In rats, the linguinal artery branching from the outer diameter artery to the central part and the thyroid artery (thyroid artery) branching from the external carotid artery to the inner carotid artery were ligated by electrocautery. At this time, 30 minutes before arterial ligation, one hour after ligation, and one hour after removal of the ligation, the extract of Ilex japonicus (50, 100, or 200 ㎎ / kg, respectively) or MK-801 (1 mg / kg) as a positive control group was fed. kg). Rat...
Embodiment 3
[0066] Embodiment 3 Passive avoidance test (passive avoidance test; Stepthrough) by rats and mice memory and learning
[0067] ICR mice (BioLinkCo. Korea) were used as experimental animals. After a period of adaptation period after purchase, they were fed with holly (25, 50 or 100 mg / kg) on the first day of the experiment (day 0) and used 30 minutes later. 15 μl (15 nmol) of 1 mMAβ(25-35) (Bachem, Switzerland) was slowly injected into the ventricles of the brain with a microsyringe equipped with a 27-gauge nuddle (Maurice Tetal., Brain Research, 1996, 706: 181-193). Within one week thereafter, feed 25, 50 and 100 mg / kg of Ilex japonicus extract once a day respectively, and place the animals in open boxes step by step through the device (Stepthrough apparatus) (Gemini II avoidance system, Sandiego Instrument) Three open area applicable tests. On the 7th day, feed the Ilex japonicus extract (25, 50 or 100mg / kg), place the animal in the bright box after 30 minutes, open th...
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