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Diagnostic Metabolic Markers Differentiating Coronary Atherosclerosis from Stable Angina

A technology for coronary atherosclerosis and stable angina pectoris, applied in the field of biochemistry, to achieve the effect of improving diagnostic convenience, high sensitivity, and promoting standardization

Active Publication Date: 2018-06-12
齐炼文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] No one has used plasma metabolite levels to diagnose coronary heart disease

Method used

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  • Diagnostic Metabolic Markers Differentiating Coronary Atherosclerosis from Stable Angina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening characterization of differential metabolites in plasma between patients with coronary atherosclerosis and patients with stable angina

[0029] 1. Objects and methods

[0030] 1. The source of the specimen

[0031] After obtaining the consent of the patients, the peripheral venous blood plasma of 480 patients with coronary atherosclerosis, 280 patients with stable angina pectoris and 350 healthy people in Jiangsu Provincial People's Hospital from September 2010 to June 2015 were collected. Confirmed by coronary angiography. The age and sex of healthy people were matched with patients with coronary atherosclerosis and stable angina pectoris. All patients with coronary atherosclerosis, patients with stable angina pectoris and healthy people have normal heart, lung, liver, kidney and hematopoietic functions.

[0032] The time of blood collection was in the morning on an empty stomach.

[0033] 2. Main reagents

[0034] Acetonitrile and formic acid ...

Embodiment 2

[0055] Example 2: Construction of ROC curve to verify the ability of 8 differential metabolites to diagnose coronary atherosclerosis and stable angina pectoris

[0056] Receiver operating curve (ROC) method was used for verification, and the expression levels of differential metabolites in the plasma of patients with stable angina pectoris and patients with coronary atherosclerosis were used to diagnose and distinguish patients with stable angina pectoris and patients with coronary atherosclerosis Ability. The results showed that phosphorylcholine, palmitoylethanolamine, phytosphingosine, phosphatidylcholine, ethyl chenodeoxycholine, lysophosphatidylcholine (16:0), lysophosphatidylcholine (18:2) and phosphatidylinositol (20:4 / 0:0), these 8 differential metabolites have a strong ability to diagnose patients with stable angina pectoris and patients with coronary atherosclerosis, and the area under the ROC curve (AUC) is greater than 0.7, which has clinical diagnostic significan...

Embodiment 3

[0065] Embodiment 3: the preparation of detection kit

[0066] A detection kit has been prepared based on the metabolic markers provided by the present invention, and the kit includes the following components:

[0067] Standards for metabolic markers: including phosphorylcholine, palmitoylethanolamine, phytosphingosine, phosphatidylcholine, ethylchenodeoxycholic acid, lysophosphatidylcholine (16:0), lysophosphatidylcholine (18:2) and phosphatidylinositol (20:4 / 0:0), each standard product is packaged separately;

[0068] Plasma metabolite extraction solvent: 100% acetonitrile and 20% acetonitrile aqueous solution (for UPLC-Q / TOF-MS sample preparation); a mixed solution of methanol, chloroform and water in a ratio of 2.5:1:1, methoxyaminopyridine and N-Methyl-N-trimethylsilyl trifluoroacetamide (for GC-Q / MS sample preparation); 20% acetonitrile aqueous solution can be used as dissolved standard in UPLC-Q / TOF-MS screening and characterization Solvent; in GC-Q / MS screening and c...

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Abstract

Disclosed is a metabolic marker for diagnosing and distinguishing coronary atherosclerosis and stable angina. The metabolic marker comprises one or more of the following components: a phosphorylcholine, a palmitoylethanolamine, a phytosphingosine, a phosphatidylcholine, a ethylchenodeoxycholic acid, a blood-soluble phosphatidylcholine (16:0), a blood-soluble phosphatidylcholine (18:2), and a phosphatidylinositol (24:0 / 0:0). Single-use of each of the components for diagnosing and distinguishing a patient with stable angina and a patient with coronary atherosclerosis results in an ROC curve with an area under the curve (AUC) of greater than 0.7 in every case, providing diagnostic significance in a clinical setting. Using the components in combination increases the AUC with every additional component in the combination, achieving the highest AUC of 0.985 when all 8 components are used. An optimal cutoff value provides a sensitivity and specificity of 97.4% and 98.0% respectively. The metabolic marker can accurately diagnose and distinguish coronary atherosclerosis and stable angina, offering good accuracy, sensitivity, and specificity.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to metabolic markers for diagnosis and classification of coronary heart disease, in particular to a group of metabolic markers for diagnosing and distinguishing coronary atherosclerosis and stable angina pectoris. Background technique [0002] Coronary heart disease, also known as ischemic heart disease, involves atherosclerosis in the arteries supplying myocardial blood, that is, coronary atherosclerotic lesions cause vascular lumen narrowing or plaque formation or even rupture and complete blockage, resulting in myocardial ischemia and hypoxia. Or necrosis leads to cardiac ischemic disease, which leads to a series of serious cardiovascular events such as clinical angina pectoris and myocardial infarction. Coronary heart disease is the main killer of human health. It has the characteristics of high incidence rate, high disability rate, high recurrence rate, high mortality rate, and many co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/02G01N33/50
Inventor 齐炼文朱伟秦勇陈彦
Owner 齐炼文