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Health cordyceps sinensis and mulberry juice amber-colored dense wine rich in DNJ

A technology of cordyceps and mulberry juice, which is applied in the field of health care amber thick wine with cordyceps and mulberry juice, to achieve the effects of lowering blood sugar, preventing bacterial infection, and enhancing human immunity

Active Publication Date: 2016-04-20
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] By searching the existing technologies at home and abroad, it is found that there is no literature report on cultivating Cordyceps mycelia fermentation broth with mulberry leaves as a medium, and using this as the main ingredient to prepare DNJ-rich Cordyceps mulberry juice health-care amber thick wine

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0029] The preparation method of the lactic acid bacteria seed solution in step 4) is as follows: activate the lactic acid bacteria with MRS solid medium, cultivate them at 30°C for 28 hours to obtain activated lactic acid bacteria, scrape an appropriate amount of them with an inoculation loop and inoculate them into 200 mL of MRS liquid medium, and cultivate them at 28°C for 48 hours , the first-grade lactic acid bacteria seed solution is prepared, and the first-grade lactic acid bacteria seed solution is inoculated into 2L of MRS liquid medium at 28° C. for 48 hours at 28° C. to obtain the second-grade lactic acid bacteria seed solution;

[0030] The lactic acid bacteria is Lactobacillus brevis (China Industrial Microorganism Culture Collection and Management Center, strain number CICC20014).

[0031] The activation method of the high activity dry yeast described in step 4) is: according to the mass ratio of yeast and glucose solution of 1:10, yeast is added to the glucose so...

Embodiment 1

[0039] 1) Wash the fresh and tender mulberry leaves, and blanching at 100°C for 10 seconds;

[0040] 2) At 45°C, add 125 parts by weight of mulberry leaves to pulp at a ratio of 1:1 to obtain mulberry leaf pulp, add 0.025 cellulase and 0.019 hemicellulase, enzymatically hydrolyze at 55°C for 5 hours, and plate and frame Press filter to extract juice to get mulberry juice;

[0041] 3) add its quality 1% glucose, 0.3% KH to step 2) in the gained mulberry juice 2 PO 4 , 0.15% MgSO 4 ·7H 2 O, 0.01% Vitamin B 1 , 0.2% yeast extract, sterilized, inoculated with 55 parts by weight of the Cordyceps seed liquid, cultivated at 23°C for 7 days, to obtain the Cordyceps mycelium fermentation liquid;

[0042] 4) Concentrate the Cordyceps mycelium fermentation liquid obtained in step 3) to 1 / 2 of the original volume by a falling film evaporator, add 90 parts by weight of white sugar to adjust the sugar content, keep warm at 18°C ​​for 2 hours, and add 14.8 parts by weight of lactic acid...

Embodiment 2

[0044] Embodiment 2, with embodiment 1, the difference is,

[0045] 2) At 40°C, add 210 parts by weight of mulberry leaves to pulp at a ratio of 1:1.2 to obtain mulberry leaf pulp, add 0.030 cellulase and 0.020 hemicellulase, enzymatically hydrolyze at 50°C for 6 hours, and plate and frame Press filter to extract juice to get mulberry juice;

[0046] 3) Add 1% glucose by mass to the mulberry juice obtained in step 2), sterilize, inoculate the Cordyceps seed liquid with 65 parts by mass, and cultivate at 23°C for 7 days to obtain the Cordyceps mycelium fermentation liquid;

[0047] 4) Add 105 parts by weight of white granulated sugar directly to the fermented broth of Cordyceps mycelia obtained in step 3) to adjust the sugar content, keep it warm at 25°C for 2 hours, add 15.2 parts by weight of lactic acid bacteria seed liquid, and after anaerobic fermentation for 42 hours, add activation Good high-activity dry yeast with a weight part of 1.4, after 5 days of anaerobic main fe...

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Abstract

The invention relates to a health cordyceps sinensis and mulberry juice amber-colored dense wine rich in DNJ. The wine is prepared by adopting the following steps: thoroughly cleaning tender mulberry leaves, blanching the thoroughly cleaned leaves, adding water for pulping, and carrying out enzymolysis, and filtering to obtain mulberry juice; adding glucose, yeast extract and the like for sterilization, inoculating cordyceps taishanensis, and culturing to obtain cordyceps sinensis fermentation liquor; concentrating with a falling-film evaporator or adding white granulated sugar to regulate the sugar degree without concentrating, adding a lactic acid bacteria seed solution and high-activity dried yeast, carrying out anaerobic fermentation, filtering, clarifying, adding konjac oligosaccharides for blending, and filtering through a biofilm for sterilizing, so as to obtain the health mulberry juice amber-colored dense wine. The health mulberry juice amber-colored dense wine is brightly amber, sticky and smooth in taste and full-bodied in ester fragrance, can reduce blood glucose, relieve fatigue, regulate intestines and stomach, clear internal heat, dissipate heat, inhibit bacteria and inflammation and improve the immune capability of human bodies, wherein the content of cordyceps polysaccharide in the wine is larger than 0.41g / 100 mL, the content of cordycepin in the wine is larger than 0.05 g / 100 mL, the content of DNJ substances is larger than 0.08 g / 100 mL, and the viscosity is larger than 200 mPa.s.

Description

technical field [0001] The invention belongs to the technical field of food processing and brewing, and in particular relates to DNJ-rich cordyceps mulberry juice health-care amber thick wine. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is a model species of Ascomycota subphylum, Ergotomycetes, Ergotaceae, and Cordyceps genus. Cordyceps militaris is rich in nutrients and functional active substances such as cordycepin, cordycepic acid, cordyceps polysaccharide, superoxide dismutase (SOD), adenosine, ergosterol, etc., which can resist fatigue, relieve aging, and improve human immunity , anti-cancer, tumor prevention and so on. It is different from general nutritional supplements in that it regulates the balance of human body functions as a whole, mobilizes the internal vitality of the body, regulates human metabolism, improves human immunity, and promotes the normalization of all internal organ functions. The nutrient components pro...

Claims

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Application Information

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IPC IPC(8): C12G3/06C12G3/02C12N1/14C12R1/645C12R1/24C12R1/865
CPCC12G3/02C12G3/06C12N1/14
Inventor 汪超刘璐汪月银卢忠诚李冬生石勇徐宁胡勇周梦舟李玮
Owner HUBEI UNIV OF TECH
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