A method for extracting 2,3-butanediol from fermentation broth

Abstract

The invention discloses a method of extracting 2,3-butanediol from fermentation liquid, wherein the method includes the following steps: 1) mixing a slurry making component, a synergist, and a less amount of the fermentation liquid uniformly under an alkaline condition to obtain a fermentation liquid pretreatment agent; 2) adding the fermentation liquid pretreatment agent to the fermentation liquid, performing a mixing reaction, regulating pH value, allowing the mixture to stand to separate a liquid phase, and collecting the liquid phase to obtain pretreated fermentation liquid; 3) adding soluble inorganic salt to the pretreated fermentation liquid and dissolving the inorganic salt and mixing the inorganic salt uniformly with the pretreated fermentation liquid, adding low-carbon alcohol and / or polyols, fully mixing the mixture, allowing the mixture to stand for layering, collecting a supernatant liquid, and purifying and extracting the supernatant liquid to obtain the 2,3-butanediol. The method can effectively remove biomacromolecules, such as protein and lipids that dissolved out from bacteria, from the fermentation liquid, which is beneficial to implementation of two-aqueous-phase extraction, rectification and the like purifying operations in next. The method is suitable for large-scale production application.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a separation and extraction method for preparing 2,3-butanediol by a biological fermentation method. Background technique [0002] 2,3-butanediol can be used as a potential platform compound to replace traditional platform compounds for large-scale synthesis of methyl ethyl ketone (excellent solvent) and 1,3-butadiene (widely used in synthetic rubber, polyester and Polyurethane and other fields). In addition, 2,3-butanediol can also be used to prepare inks, perfumes, fumigants, moisturizers, softeners, plasticizers, explosives and pharmaceutical chiral carriers, etc., 2,3-butanediol Oxidation products 3-hydroxybutanone and diacetyl can be used as edible spices; at the same time, because of their high calorific value, which is equivalent to ethanol and methanol, they can be used as fuel additives; 2,3-butanediol can be produced after esterification The precursor of polyurethane f...

AI Technical Summary

Problems solved by technology

[0003] In the process of biological fermentation to prepare 2,3-butanediol, glucose is usually used as a carbon source, so there are many by-products in the fermentation process, and the fermentation broth system is relatively complicated, which increases the extraction and purification of 2,3-butanediol products difficulty

2,3-Butanediol has two hydroxyl groups, is extremely hydrophilic, and has a boiling point of 180°C at atmospheric pressure. Conventional vacuum distillation, overevaporation, and membrane evaporation cannot achieve good extraction results.

Although 2,3-butanediol is an organic molecule, because it contains two hydroxyl groups, it is easy to form hydrogen bonds, and the molecule is relatively small and has strong hydrophilicity. The theoretical adsorption capacity of alcohol is very low, and the industrial application prospect is not ideal

[0005] At present, the separation method of 2,3-butanediol is mainly based on the molecular characteristics of 2,3-butanediol, using physical or chemical methods to carry out targeted concentration and purification, but the research results at this stage show that, The actual separation effect is not ideal, the main reason is that in the fermentation broth of 2,3-butanediol, sugars, fermentation by-products, bacteria dissolved proteins, lipids and other biomacromolecules, under certain conditions, have Strong binding effect, so it is difficult to achieve obvious separation effect only by a single treatment