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Preparation method of gold nano-composite targeting drug delivery system

A drug delivery system and compound target technology, applied in the field of nano-biomedicine, can solve the problems of poor drug loading and targeting, and achieve the effects of increasing drug loading, improving therapeutic effect, and good targeting effect

Inactive Publication Date: 2016-06-01
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the problem of poor targeting of mesoporous silica-coated gold nanocarriers in the prior art, and to provide a novel targeting scheme, which can be achieved by mesoporous silica-coated gold nanoparticles. The synergistic treatment of photothermal therapy and chemotherapy can effectively target the tumor site, accumulate rapidly in cancer cells, and be used in the treatment of tumors

Method used

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  • Preparation method of gold nano-composite targeting drug delivery system
  • Preparation method of gold nano-composite targeting drug delivery system
  • Preparation method of gold nano-composite targeting drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Preparation of gold nanoparticles seeds

[0032] Mix 5ml, 0.2M cetyl ammonium bromide solution and 5ml, 0.0005M chloroauric acid solution, stir quickly and add to 0.6ml, 0.010M sodium borohydride solution at one time, stir vigorously for 2min, at 20°C Stand still for 2 h to obtain gold nanoparticle seed solution.

[0033] 2) Preparation of gold nanorods

[0034] To 5ml, 0.2M cetyl ammonium bromide solution, add 5.0ml, 0.001M chloroauric acid solution, 0.15ml, 0.0040M silver nitrate solution and mix, then add 80μl, 0.1M ascorbic acid solution, and then add 10μl After mixing the seed solution evenly, let it stand at 20°C for 12 hours, and centrifuge and wash once to obtain gold nanorod GNRs;

[0035] 3) Preparation of mesoporous silica-coated gold nanorods

[0036]Take 10ml of gold nanorod solution, adjust the pH to 10 with sodium hydroxide solution, then add 30 μl of 20% volume fraction TEOS / methanol solution every half hour (total three times), stir at 20°C for 12...

Embodiment 2

[0047] 1) Preparation of gold nanoparticles seed solution

[0048] Mix 5ml, 0.1M cetyl ammonium bromide solution and 10ml, 0.001M chloroauric acid solution, stir quickly and add it to 1ml, 0.010M sodium borohydride solution at one time, stir vigorously for 2min, and stand at 30°C Set aside for 2.5h to obtain gold nanoparticle seed solution.

[0049] 2) Preparation of gold nanorods

[0050] To 5ml, 0.1M cetyl ammonium bromide solution, add 5.0ml, 0.01M chloroauric acid solution, 0.05ml, 0.010M silver nitrate solution, mix, add 100μl, 0.1M ascorbic acid solution, then add 12μl After the seed solution, stand at 30°C for 24 hours, and centrifuge once to obtain gold nanorods;

[0051] 3) Preparation of mesoporous silica-coated gold nanorods

[0052] Take 10ml of gold nanorod solution, add 100μl, 0.1M sodium hydroxide solution, stir and mix, then add 20μl of 20% volume fraction TEOS / methanol solution every half hour (four times in total), and stir at 40°C for 48h to obtain , and...

Embodiment 3

[0060] 1) Preparation of gold nanoparticles seed solution

[0061] Mix 5ml, 0.1M cetyl ammonium bromide solution and 8ml, 0.005M chloroauric acid solution, stir quickly and add to 0.7ml, 0.010M sodium borohydride solution at one time, stir vigorously for 2min, at 25°C After standing still for 3 hours, the gold nanoparticle seed solution was obtained.

[0062] 2) Preparation of gold nanorods

[0063] Add 0.25ml, 0.0040M silver nitrate solution to 5ml, 0.2M cetyl ammonium bromide solution and mix, then add 85μl, 0.1M ascorbic acid solution, then add 20μl seed solution, and let it stand at 25°C for 20h. Centrifuge twice to obtain gold nanorods;

[0064] 2) Preparation of mesoporous silica-coated gold nanorods

[0065] Take 10ml of gold nanorod solution, add 150μl, 0.1M sodium hydroxide solution, mix well, then add 30μl of 20% TEOS / methanol solution every half hour (four times in total), stir slowly for 36h, and then use deionized Centrifugal washing with water and methanol se...

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Abstract

The invention discloses a preparation method of a gold nano-composite targeting drug delivery system. The preparation method comprises the following steps: sequentially adding a chloroauric acid solution and a silver nitrate solution into a hexadecyl ammonium bromide solution, mixing uniformly, then sequentially adding an ascorbic acid solution and a gold nano-particle seed solution, and centrifuging to obtain a gold nano-bar; adding ethyl orthosilicate to obtain a mesoporous silicon oxide nano-material; adding 3-aminopropyl triethylsilane to obtain aminated GNRs@mSiO2; adding a thionyl chloride solution of melphalan to obtain aminated GNRs@mSiO2-MEL; and adding HA-RGD to obtain GNRs@mSiO2-HA-RGD-MEL. According to the preparation method, gold nano-particles with an effect of photothermal therapy are effectively combined with an anti-cancer drug, namely melphalan, thereby realizing combined therapy of tumor thermotherapy and chemotherapy and increasing the therapeutic effect; and by utilizing mesoporous silicon oxide ducts for efficiently loading drugs, the drug loading capacity can be increased.

Description

technical field [0001] The invention relates to a preparation method of a gold nanocomposite targeted drug delivery system, which belongs to the field of nano biomedicine. Background technique [0002] Cancer is a disease with a high mortality rate, which has attracted widespread attention. Traditional cancer treatment methods include: chemotherapy, radiotherapy, and hyperthermia. These methods have certain curative effects, but they may also kill normal immune cells while killing cancer cells, reducing the body's immunity. Photothermal therapy belongs to a kind of physical therapy. Its basic principle is: after exogenous photothermal agent targeting tumor tissue, laser radiation is used to make the photothermal agent generate high heat through photothermal conversion, destroying and eliminating tumor cells. Among the many materials that can be used in photothermal therapy, noble metal nanomaterials, such as gold nanoparticles, have localized surface plasmon oscillation (LS...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K31/196A61K41/00A61P35/00
CPCA61K41/0052A61K31/196A61K2300/00
Inventor 许沛虎周汇敏徐海星黄志军张凌溪郭玉凤李依萍吴峰政杨夏雯
Owner WUHAN UNIV OF TECH
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