Method for preparing salt-resistant alkali-resistant special algal biological water-soluble fertilizer by enzymatic hydrolysis fermentation

The invention relates to a fermentation method and a salt-alkali resistance technology, which is applied in the field of the preparation of salt-alkali-resistant seaweed biological water-soluble fertilizers by an enzymatic hydrolysis and fermentation method. Effect

Inactive Publication Date: 2016-06-01
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, seaweed fertilizers and microbial fertilizers are relatively new types of improved products. They have good application prospects in the fields of improving the soil environment and utilizing salinized land. They are not only low in cost, but also have the advantages of no secondary pollution. Many experts and scholars have recognized seaweed The improvement effect of f

Method used

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specific Embodiment approach

[0020] The present invention provides a specific embodiment of a method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymatic hydrolysis and fermentation, which specifically includes the following steps:

[0021] (1) Seaweed pretreatment: wash the seaweed with clean water, remove impurities, drain; dry at 80-90°C, finally grind and sieve;

[0022] (2) Ultrasonic-assisted enzymolysis: add water 8 to 10 times the mass of seaweed, and adjust the pH to 6.8 to 7.2; Ultrasonic-assisted enzymatic hydrolysis at 60°C for 2-4 hours;

[0023] (3) Fermentation broth preparation: add 3.0% to 5.0% glucose, 2.0% to 3.0% yeast extract, 0.3% to 0.5% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;

[0024] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60% to 70%, inoculate Bacillus pyrolyticum and Aspergillus niger, and inocul...

Embodiment 1

[0030] A method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymolysis and fermentation, the specific preparation steps are as follows:

[0031] (1) Seaweed pretreatment: wash the seaweed with clean water, remove impurities, and drain; dry at 85°C, finally grind into powder, and pass through a 40-mesh sieve;

[0032] (2) Ultrasonic-assisted enzymolysis: add water 10 times the mass of seaweed, adjust the pH to 6.8-7.2; add 3% glucoamylase and 2% non-starch polysaccharase according to the mass ratio of seaweed, and ultrasonic-assisted enzymolysis at 40-60°C for 2 hours, Ultrasonic power 400w, frequency 25kHz;

[0033] (3) Fermentation broth preparation: Add 5.0% glucose, 3.0% yeast extract, 0.5% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;

[0034] (4) Aerobic fermentation: select a liquid fermentation tank with a liquid volume of 60% to 70%...

Embodiment 2

[0039] A method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymolysis and fermentation, the specific preparation steps are as follows:

[0040] (1) Seaweed pretreatment: wash the seaweed with clean water, remove impurities, and drain; dry at 85°C, finally grind into powder, and pass through a 40-mesh sieve;

[0041] (2) Ultrasonic-assisted enzymolysis: add water 8 times the mass of seaweed, adjust the pH to 6.8-7.2; add 2% glucoamylase and 1% non-starch polysaccharase according to the mass ratio of seaweed, and ultrasonic-assisted enzymolysis at 40-60°C for 4 hours, Ultrasonic power 400w, frequency 25kHz;

[0042] (3) Fermentation broth preparation: Add 3.0% glucose, 2.0% yeast extract, 0.3% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;

[0043] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60% to 70%, in...

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Abstract

The invention discloses a method for preparing salt-resistant alkali-resistant special algal biological water-soluble fertilizer by enzymatic hydrolysis fermentation, characterized by comprising: (1), pretreating algae; (2), carrying out ultrasonic-assisted enzymatic hydrolysis; (3), preparing fermentation broth; (4), aerobically fermenting; (5), fermenting in two steps; (6), filtering; (7), concentrating. refractory macromolecular substances in algae are degraded into small molecule polysaccharides, monosaccharides and nitrogen-bearing compounds by means of composite enzymatic hydrolysis and multi-bacteria co-fermentation, a great amount of soil beneficial bacteria and various specially-effective metabolites are generated, bioactivity of the algal fertilizer is improved, strong alkali, high temperature and high pressure are avoided being destroyed during algal fertilizer production, and rich nutrients in algae are fully reserved. This product integrates natural nutrients, antibiotics and plant hormones, has the composite effect of 'triple in one', organic fertilizer, microbial fertilizer and trace element fertilizer, is effective in improving saline and alkali soil and has a promising application prospect.

Description

technical field [0001] The invention belongs to the field of fertilizer production, and relates to a method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymolysis and fermentation. Background technique [0002] At present, soil salinization is very serious, which has brought a great impact on agriculture, which is not conducive to the planting of plants, and has brought adverse effects on agricultural resources. In my country's 100 million square kilometers of arable land, about 10% of the land is saline soil. In addition, there are more than 2,000 hectares of salt wasteland, most of which have not been cultivated. How to reclaim and utilize these salinized soils, expand planting area, and increase national income is one of the important issues in current agricultural production. At present, the national government and scientific researchers are paying attention to the restoration of salinized soil, actively carrying ou...

Claims

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Application Information

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IPC IPC(8): C05G3/04C05F17/00C05G3/80
CPCC05B7/00C05F17/50C05G3/80C05D3/00C05D5/00C05F11/00C05F11/08Y02W30/40
Inventor 单俊伟董书阁王海华但丽霞马栋
Owner 青岛海大生物集团股份有限公司
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