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Micro-electrolysis system and its preparation method and application

A micro-electrolysis and system technology, applied in the interdisciplinary field of bio-nano technology, can solve the problems of sewage treatment needs to be improved, narrow scope of application, high cost of removal, etc., and achieve the effect of good industrial application prospects

Active Publication Date: 2018-05-29
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional hexavalent chromium reduction methods include: (1) Oxidation-reduction process: direct current reduction method, reducing agent addition method such as sodium thiosulfate, ferrous sulfate, but this method needs to input some chemical substances, which is easy to cause secondary pollution; (2) Coagulation process: Chromium can be combined with azo organic ligands in a complex state, and the structure is very stable, but the removal cost is high and the scope of application is narrow; (3) Biological chromium removal technology: Many microorganisms can Reducing hexavalent chromium to trivalent chromium under aerobic or anaerobic conditions, and then adsorbing through extracellular viscous substances, or participating in intracellular metabolism, of course, the effect is not very satisfactory
[0005] Therefore, the current technology of sewage treatment still needs to be improved.

Method used

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  • Micro-electrolysis system and its preparation method and application
  • Micro-electrolysis system and its preparation method and application
  • Micro-electrolysis system and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Genetic engineering of the p8 capsid protein around the M13 bacteriophage

[0054] The N-terminal sequence of 2700 copies of p8(pVIII) protein in the wild-type M13 phage (purchased from NEB) is N-Ala-Glu-Gly-Asp-Asp-Pro-...(N-AEGDDP-). In this example, the strongly negatively charged polypeptide sequence AEEEAAEEDP was designed, and its gene sequence GAAGAAGAAGCGGCGGAAGAG (SEQ ID NO: 6) was inserted into the N-terminus of the wild-type M13 phage to obtain a gene with strong adsorption to multiple metal ions at the same time Engineering phage M13-6ED. The specific operation method is as follows:

[0055] First, the M13KE phage vector (Mao et al. Virus-Based Toolkit for the DirectedSynthesis of Magnetic and Semiconductor Nanowires. Science. 2004, 303: 213-217) was digested with Pst I (15U / μl, purchased from TaKaRa Biotechnology, Dalian) , the reaction system is: Pst I, 1 μl; 10×H buffer, 2 μl; M13KE phage carrier, 1 μg; add sterile water to a total volume of ...

Embodiment 2

[0058] Example 2: Construction of Fe-Ni@M13 micro-electrolysis system

[0059] Using the method described in Example 1, the recombinant M13 phage M13-6ED obtained in Example 1 was amplified. Take an appropriate amount of recombinant M13 phage obtained by amplification, and supplement the volume to 200 μL with TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH=7.5). In the obtained mixture, the titer of the recombinant M13 phage is 10 11 pfu, and then add an equal volume (100 μL each) mixed ferrous chloride (2mM) and nickel chloride (2mM) solution to it, to treat the recombinant M13 phage on Fe 2+ and Ni 2+ After the adsorption is complete (stand still for about 5-10min), add the reducing agent NaBH 4 Solution (4mM) to the Fe in the system 2+ and Ni 2+ Perform reduction (reaction while oscillating), and react at normal temperature and pressure for about 10 minutes to construct the Fe-Ni@engineeredM13-1 micro-electrolysis system. The nano-iron and nano-nickel particles uniformly di...

Embodiment 3

[0060] Example 3: Fe-Ni@engineered M13-1 micro-electrolysis system efficiently reduces p-chloronitrobenzene and hexavalent chromium ions

[0061]In this example, the Fe-Ni@engineeredM13-1 micro-electrolysis system constructed in Example 2 was used for the reduction of p-chloronitrobenzene. Take 200 μL of p-chloronitrobenzene solution with a concentration of 1 mg / L, add 20 μL of phosphoric acid (2mol / L) to acidify the p-chloronitrobenzene solution, and then add the Fe-Ni@engineeredM13-1 micro-electrolysis system obtained in Example 2 to carry out Reduction reaction about 10min. The Fe@engineered M13-1 system (the preparation method is the same as in Example 2, the difference being that no nickel chloride solution was added), nano-iron particles, and a reducing agent were used as a control. Use high-performance liquid chromatography (chromatographic column: Gemini 5u C18110A (250 * 4.6mm); Mobile phase methanol: water=75:25, flow rate 1ml / min, carry out vacuum suction filtratio...

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Abstract

The invention provides a microelectrolysis system as well as a preparation method and an application thereof. The microelectrolysis system comprises bacteriophage M13, first metal and second metal, wherein the first metal and the second metal are distributed on the surface of the bacteriophage M13 and have a potential difference. The first metal and the second metal have the potential difference, so that the microelectrolysis system can be formed effectively and can be used for performing reductive treatment on organic pollutants or heavy metal ions in sewage, the reduction rate is high, required time is short, treatment can be performed at normal temperature and normal pressure, and the microelectrolysis system does not need a noble metal catalyst and is lower in cost.

Description

technical field [0001] The invention relates to the interdisciplinary field of bio-nano technology and the field of sewage treatment. Specifically, the invention relates to a micro-electrolysis system and its preparation method and application. Background technique [0002] Bio-nanotechnology, which uses organisms as synthetic tools, has been applied in more and more fields as a new technology, such as biochips, nano biomimetic materials, etc. Among them, the phage display technology using filamentous nano-scale M13 phage as a biological template is one of the research hotspots, and has been used in many fields such as mediating the synthesis of metal nanoparticles and nanowires, vaccine research, and catalyst preparation. [0003] Environmental pollution control is a key issue closely related to human health. It will be of great practical significance if bio-nanotechnology can be applied to the environmental restoration of organic wastewater and heavy metal pollution. Wast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/461
CPCC02F1/461
Inventor 于慧敏张帅杨继沈忠耀
Owner TSINGHUA UNIV
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