Application of sodium pyruvate in the preparation of storage solution for improving the quality of stored red blood cells and/or tissue damage after infusion

A technology of sodium pyruvate and tissue damage, which is applied in the field of medicine and can solve the problems of not being able to improve red blood cell oxidative damage, not being able to improve cytokine levels, and reducing immunogenicity

Active Publication Date: 2019-12-13
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, adding energy metabolism substrate glucose to stored red blood cells can improve red blood cell energy metabolism and effectively reduce the hemolysis rate of stored red blood cells, but it cannot improve red blood cell oxidative damage
Stowell et al. added vitamin C to stored red blood cells, which can effectively improve the survival rate of stored red blood cells after reinfusion, and can reduce their immunogenicity. The level of inflammatory response in the body (Stowell, S.R., Smith, N.H., Zimring, J.C., Fu, X., Palmer, A.F., Fontes, J., Banerjee, U. & Yazer, M.H. (2013) Addition of ascorbic acid solution to stored murine red blood cells increases post transfusion recovery and decreases microparticles and alloimmunization. Transfusion, 53, 2248-2257.)

Method used

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  • Application of sodium pyruvate in the preparation of storage solution for improving the quality of stored red blood cells and/or tissue damage after infusion
  • Application of sodium pyruvate in the preparation of storage solution for improving the quality of stored red blood cells and/or tissue damage after infusion
  • Application of sodium pyruvate in the preparation of storage solution for improving the quality of stored red blood cells and/or tissue damage after infusion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, preparation of stored erythrocytes added with sodium pyruvate

[0037] 1. Preparation of sodium pyruvate solution

[0038] Accurately weigh 35 mg of sodium pyruvate powder with an electronic analytical balance, dissolve it in 1 mL of normal saline, and prepare a sodium pyruvate solution with a sodium pyruvate concentration of 35 mg / mL. filter with a pore size bacterial filter, place in a sterile centrifuge tube, and set aside.

[0039] 2. Acquisition of red blood cells and preparation of stored red blood cells

[0040] Take mice and inject 2.5% pentobarbital sodium solution (the injection volume is calculated according to 0.3mL / 100g mouse body weight), fix the anesthetized mice on a heating plate at 37°C, and place them in a sterile ultra-clean working room. On the stage, wipe the outer surface of the abdominal cavity and thorax of the mouse with alcohol cotton balls, take blood by aseptic heart puncture, and use CPDA-1 solution as an anticoagulant and blood ...

Embodiment 2

[0042] Embodiment 2, measure the ATP content in the stored erythrocyte

[0043] The ATP content of the seven groups of stored erythrocytes obtained in Example 1 after storage for 14 days was measured by the method described in the ATP detection kit (the ATP detection kit was purchased from Beyontian Biotechnology Co., Ltd., Cat. No. S0026). Take SP Group 3 # (the final concentration of sodium pyruvate in the stored erythrocytes is 2mM) as an example, the results are as follows figure 1 shown.

[0044] figure 1 The results showed that the ATP content of the blank control group was 2.46±0.19nmol / mg protein, and the SP group 3 # The ATP content in stored red blood cells was 2.82±0.21nmol / mg protein. Compared with blank control group, SP group 3 # The ATP content in stored red blood cells was significantly increased (* indicates p<0.05). It shows that sodium pyruvate can increase the ATP level in red blood cells during the storage of red blood cells, provide substrates for e...

Embodiment 3

[0046] Embodiment 3, measure MDA content in the stored erythrocyte

[0047] Use the instruction method in the MDA detection kit (the MDA detection kit was purchased from Nanjing Jiancheng Biotechnology Co., Ltd., article number A003-1) to measure the lipid peroxidation product-propanediol of seven groups of stored red blood cells obtained in Example 1 after storage for 14 days. Aldehyde (MDA) content. Take SP Group 2 # For example, results such as figure 2 shown.

[0048] MDA is one of the most important terminal metabolites produced by the peroxidation reaction between lipids in the cell membrane and ROS. Its content reflects the intensity and speed of intracellular lipid peroxidation. Therefore, the MDA content is used to reflect the cell and An important indicator of tissue oxidative stress damage (Kwiecien S, Konturek PC, Sliwowski Z, et al. Interaction between selective cyclooxygenase inhibitors and capsaicin-sensitive afferent sensory nerves in pathogenesis of stress...

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Abstract

The invention discloses a new use of sodium pyruvate, namely application in the preparation of a storage liquid for improving stored erythrocyte quality and / or post-infusion tissue damage.The sodium pyruvate is used as an active ingredient to prepare the storage liquid for improving stored erythrocyte quality and / or post-infusion tissue damage, it is possible to improve the stored erythrocyte quality and / or post-infusion tissue damage, the therapeutic effect is significant, no side effect occurs, safety is good, and the sodium pyruvate plays an important role in studying and developing this storage liquid and has promising application prospect and great social significance.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of sodium pyruvate in preparing storage solution for improving the quality of stored red blood cells and / or tissue damage after transfusion. Background technique [0002] In clinical practice, for patients with massive bleeding caused by trauma or surgery, blood transfusion is a basic treatment method, which has saved countless lives. Because red blood cells have the function of combining and transporting oxygen and carbon dioxide, they can provide oxygen to tissues and remove carbon dioxide produced by metabolism. Therefore, blood transfusion can improve the oxygen-carrying capacity of blood, increase tissue oxygen supply, and improve oxygenation status. However, in clinical blood transfusion, it is not possible to take out the red blood cells for immediate use, and most of the red blood cells transfused by patients are red blood cells that have been stored for a period o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/12A61K35/18A61P7/08
CPCA61K35/18A61K47/12
Inventor 陈赣周虹赵莲王瑛尹玉静夏厦
Owner ACADEMY OF MILITARY MEDICAL SCI
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