Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of cross-linked hyaluronic acid dry powder and its preparation method and application

A technology of cross-linking hyaluronic acid and hyaluronic acid, which is applied in the fields of pharmaceutical formulations, toiletry preparations, cosmetic preparations, etc., and can solve the problems of large-scale industrial production, hyaluronic acid long-chain break degradation, and impact on dialysis operations. Gel use effect and other issues, to achieve the effect suitable for large-scale production, thickening and skin feeling, and convenient for clinical use

Active Publication Date: 2019-01-08
BLOOMAGE BIOTECHNOLOGY CORP LTD
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the reaction system of this method, the hyaluronic acid is dissolved in the lye and directly reacted with the crosslinking agent, which will cause the breakage and degradation of the long chain of hyaluronic acid and affect the crosslinking effect.
At the same time, what is obtained in the middle of this method is a wet precipitate containing a large amount of organic solvent and water. Although dialysis is performed after reconstitution, a relatively large amount of organic solvent will still remain in the obtained cross-linked hyaluronic acid gel, which will affect the use effect of the gel.
And dialysis operation is not easy for large-scale industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of cross-linked hyaluronic acid dry powder and its preparation method and application
  • A kind of cross-linked hyaluronic acid dry powder and its preparation method and application
  • A kind of cross-linked hyaluronic acid dry powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Accurately take 5 μl of 1,4-butanediol diglycidyl ether and add it to 100mL of sodium chloride solution (50mg / mL). After mixing, weigh 1g of sodium hyaluronate (molecular weight: 1.5 million Da) and dissolve it in the above solution. After the sodium hyaluronate is fully dissolved, gradually add 186ml of ethanol until the sodium hyaluronate precipitates a white precipitate. After the whole reaction system showed a solid-liquid suspension system, it was stirred and reacted at 30° C. for 6 hours. Then let stand and discard the supernatant. Finally, wash and dehydrate with ethanol for 3 times, then suction filter and discard the filtrate, put the wet powder in a vacuum drying oven at 25°C and dry it to make a dry cross-linked sodium hyaluronate powder. Weigh 0.2 g of the dry powder and add it to 100 ml of purified water to dissolve, and as a result, a colorless, transparent, smooth and viscous solution is obtained, with a kinetic viscosity of 350 mPa s, which is higher th...

Embodiment 2

[0040]Accurately take 10 μl of 1,4-butanediol diglycidyl ether and add it to 100 mL of sodium chloride solution (50 mg / mL). After mixing, weigh 1 g of sodium hyaluronate (molecular weight: 1.5 million Da) and dissolve it in the above solution. After the sodium hyaluronate is fully dissolved, gradually add 186ml of ethanol until the sodium hyaluronate precipitates a white precipitate. After the whole reaction system showed a solid-liquid suspension system, it was stirred and reacted at 30° C. for 6 hours. Then let stand and discard the supernatant. Finally, it was washed and dehydrated with ethanol for 3 times, and the filtrate was discarded by suction filtration. The wet powder was dried in a vacuum drying oven at 30°C to prepare a dry cross-linked sodium hyaluronate powder. Weigh 0.2 g of the dry powder and add it to 100 ml of purified water to dissolve, and as a result, a colorless, transparent, smooth and viscous solution is obtained, with a dynamic viscosity of 1020 mPa s...

Embodiment 3

[0042] Accurately take 20μl of 1,4-butanediol diglycidyl ether and add it to 100mL of sodium chloride solution (50mg / mL). After mixing, weigh 1g of sodium hyaluronate (molecular weight: 1.5 million Da) and dissolve it in the above solution. After the sodium hyaluronate is fully dissolved, gradually add 186ml of ethanol until the sodium hyaluronate precipitates a white precipitate. After the whole reaction system showed a solid-liquid suspension system, it was stirred and reacted at 30° C. for 6 hours. Then let stand and discard the supernatant. Finally, it was washed and dehydrated with ethanol for 3 times, and the filtrate was discarded by suction filtration. The wet powder was dried in a vacuum drying oven at 40°C to prepare a dry cross-linked sodium hyaluronate powder. Weigh 0.2 g of the dry powder and add it to 100 ml of purified water to dissolve, and as a result, a colorless, viscous solution with transparent particles is obtained, and its dynamic viscosity is 7800 mPa ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of hyaluronic acid, in particular to crosslinked hyaluronic acid dry powder. Hyaluronic acid is dissolved in an inorganic salt water solution premixed with a crosslinking agent; an organic solvent is added, precipitates are separated out from the hyaluronic acid, a solid-liquid suspension system is formed, and a cross-linking reaction is performed; an organic solvent is added for washing the precipitates; vacuum drying is performed, and the crosslinked hyaluronic acid dry powder is obtained. The crosslinking reaction is performed in the solid-liquid suspension state, and the gelling property is better; crosslinked hyaluronic acid gels with different concentrations can be prepared from the obtained crosslinked hyaluronic acid dry powder, and use is facilitated. After the obtained crosslinked hyaluronic acid dry powder is dissolved in water, colorless transparent gel is formed, and the crosslinked hyaluronic acid dry powder has the effects that the viscoelasticity is excellent, the dry powder can perform the function of long-acting moisture retention after being applied to cosmetics, physical wrinkle filling is realized, active ingredients are released slowly, the cosmetics cosmetic is thickened, the skin feeling during use is improved and the like.

Description

technical field [0001] The invention relates to the technical field of hyaluronic acid, in particular to a dry powder of cross-linked hyaluronic acid, as well as its preparation method and application. Background technique [0002] Hyaluronic acid is widely distributed in various parts of the human body, and the skin also contains a large amount of hyaluronic acid. As the human body ages, the content of hyaluronic acid in the skin gradually decreases. Hyaluronic acid has been widely used in cosmetics. It can improve skin nutrition metabolism, make skin soft, smooth, wrinkle-free, increase elasticity, and prevent aging. It is also a good transdermal absorption accelerator while moisturizing. However, when the amount is added in a large amount, it is easy to draw and get muddy during the application process, and the skin after application sweats or is in a relatively humid environment, and is prone to stickiness. Cross-linked or modified hyaluronic acid has different rheolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24A61K8/73A61K47/36A61L27/20A61L27/52A61Q19/00A61Q19/08C08L5/08
CPCA61K8/735A61K47/36A61L27/20A61L27/52A61L2400/06A61Q19/00A61Q19/08C08J3/24C08J3/247C08J2305/08
Inventor 王冠凤郭学平石艳丽魏健钱晓路王成山耿凤
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com