Pharmaceutical composition of amikacin sulfate and medical application of pharmaceutical composition

A technology of amikacin sulfate and a composition, applied in the field of biomedicine, can solve the problems such as the related reports on chronic myeloid leukemia and the like, and achieve the effect of improving the therapeutic effect

Inactive Publication Date: 2016-07-06
WENZHOU JUYICHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there has been no report on the correlation between the pharmaceutical composition of amikacin sulfate and chronic myelogenous leukemia

Method used

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  • Pharmaceutical composition of amikacin sulfate and medical application of pharmaceutical composition
  • Pharmaceutical composition of amikacin sulfate and medical application of pharmaceutical composition
  • Pharmaceutical composition of amikacin sulfate and medical application of pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0019] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.

[0020] Separation method: (a) Grind the dry root and rhizome (5kg) of Radix isatidis, extract with 75% ethanol under hot reflux (20L×3 times), combine the extracts, concentrate until no alcohol smell (4L), and then use petroleum ether ( 4L×3 times), ethyl acetate (4L×3 times) and water-saturated n-butanol (4L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b ) Ethyl acetate extract in step (a) is removed with AB-8 type macroporous resin, first elute 8 column volumes with 6% ethanol, then elute 8 column volumes with 70% ethanol, collect 70% eluent Re...

Embodiment 2

[0023] Example 2: Therapeutic effect on chronic myelogenous leukemia

[0024] 1. Materials and Instruments

[0025] K562 cells were provided by Shanghai Institute of Biological Cells, Chinese Academy of Sciences. Compound (I) is self-made, and the HPLC normalized purity is greater than 98%. RPMI-1640 dry powder medium was purchased from Gibco. Calf serum was purchased from Hangzhou Sijiqing Institute of Bioengineering Materials. Penicillin and streptomycin were purchased from Huabei Pharmaceutical Factory. Glutamine, MTT, DMSO were purchased from AMRESCO. Annexinv-FITC cell apoptosis detection kit was purchased from Nanjing KGI Biotechnology Development Co., Ltd. MOPS, Rnasin, agarose, olfactory ethidium, bromophenol blue Huamei Bioengineering Company Huamei Bioengineering Company.

[0026] CO 2 Incubator (Thermo Company, USA), ultra-clean bench (Suzhou Purification Equipment Factory), magnetic stirrer (Shanghai Nanhui Telecommunication Equipment Factory), inverted micr...

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PUM

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Abstract

The invention discloses a pharmaceutical composition of amikacin sulfate and its medical application. The pharmaceutical composition of amikacin sulfate provided by the invention contains amikacin sulfate and a dry root and rhizome from Radix isatidis The natural product compound (I) with a novel structure isolated from , when amikacin sulfate and the natural product compound (I) act alone, the therapeutic effect on chronic myeloid leukemia is weak; The curative effect of myeloid leukemia is significantly improved, and it can be developed into a drug for treating chronic myeloid leukemia, which has outstanding substantive features and significant progress compared with the prior art.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the treatment of chronic myeloid leukemia, in particular to a pharmaceutical composition of amikacin sulfate and its therapeutic effect on chronic myeloid leukemia. Background technique [0002] Amikacin sulfate is a semi-synthetic aminoglycoside antibiotic with a similar antibacterial spectrum to gentamicin, and is effective against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Proteus. This product is also effective against other aminoglycoside antibiotic-resistant strains. It is mainly used clinically for pyelonephritis, urinary tract infection, respiratory tract and lung infection, and sepsis caused by sensitive bacteria. [0003] Chronic myeloid leukemia is a malignant tumor that affects the blood and bone marrow. It is characterized by the production of a large number of immature white blood cells, which accumulate in the bone marrow and inhibit the normal hema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/14A61K31/4375A61K31/7036A61K36/195A61K36/315A61P35/02
CPCC07D471/14A61K31/4375A61K31/7036A61K36/195A61K36/315A61K2236/333A61K2236/51A61K2236/55
Inventor 不公告发明人
Owner WENZHOU JUYICHUANG TECH CO LTD
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