Special compound microorganism bacterium agent capable of eliminating continuous cropping obstacles of radix pseudostellariae and preparation method of special compound microorganism bacterium agent
A compound microbial inoculum and a technology for continuous cropping obstacles are applied in the special compound microbial inoculum for eliminating obstacles to continuous cropping of Radix Radix et Rhizoma and the field of preparation thereof, which can solve the problems of deterioration of soil environment and inability of beneficial bacteria to colonize, and achieve activation of soil nutrients, roots and roots. The effect of good colonization ability and improvement of soil physical and chemical properties
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Embodiment 1
[0041] Example 1 Screening and identification of indigenous antagonistic bacteria
[0042] Pseudomonas selective medium and Bacillus selective medium were used to isolate Pseudomonas and Bacillus from the rhizosphere of Radix Pseudostellariae, and inorganic medium (containing higher concentrations of autotoxic substances in Radix Pseudostellariae) was used to isolate Toxin-degrading bacteria were isolated.
[0043] Randomly selected single colonies on the above-mentioned different media were used to face off on PDA plates to evaluate their antagonistic effects on heterophyllosis-specific pathogenic bacteria (such as Fusarium oxysporum, Fusarium moniliforme, and ankle mold, etc.). Bacteria with strong antagonistic ability were identified by 16srRNA gene or 16s-23srRNA interval PCR identification for molecular identification. 16srRNA gene PCR amplification upstream and downstream primers are: 27f (5'-AGAGTTTGATCCTGGCTCAG-3') and 1522r (5'-AAGGAGGTGATCCAGCCGCA-3'); 16s-23srRNA g...
Embodiment 2
[0050] The preparation of embodiment 2 special-purpose composite microbial bacterial agents
[0051] A kind of special-purpose composite microbial bacterial agent described in the present invention is formed by mixing general-purpose beneficial bacteria and native beneficial bacteria in a ratio of 4:6 by volume, and the specific preparation method is as follows:
[0052] 2.1EM composite bacterial solution preparation
[0053] Add 300g of brown sugar and 50mL of MS mother liquor to 18L of well water (tap water needs to be left for at least 2 days before use), then add 1L of activated EM liquid bacteria, and place it in a room at 35-37°C for 2-3 weeks of closed fermentation. From the second day onwards, you can open the airtight container mouth to deflate and reduce pressure once every other day. When you open the container mouth, you can smell a sweet and sour smell, which becomes more and more obvious. The successful finished fermentation broth has a sweet and sour taste, pH3...
Embodiment 3
[0063] Example 3 Evaluation of Field Effect of Special Compound Microbial Inoculant to Prevent and Control Pseudostellaria Pseudostellariae Continuous Cropping Obstacles
[0064] First, the prepared special compound microbial agent is fermented together with organic materials to prepare bio-organic fertilizer, which is used as base fertilizer to pre-improve the soil of Pseudostellariae heterophylla, and then the plots are prepared to plant Pseudostellaria heterophylla, and proper amount shall be applied from the seedling stage of Pseudostellaria heterophylla Composite microbial liquid inoculum specially used for root irrigation and topdressing, and the yield difference was compared during the harvest period. The specific preventive measures are as follows:
[0065] 3.1 Preparation of microbial organic base fertilizer
[0066] Mix the organic materials bean dregs, rice bran, dried pig manure, fish meal, and brown sugar in a weight ratio of 2:1:1:1:0.03, then add an appropriate...
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