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Preparation method and application of nanoscale bacterial cellulose membrane

A bacterial cellulose membrane and nano-scale technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, skin care preparations, etc., can solve the problems of long fermentation cycle, low degree of mechanization, and reduced price of end products. Achieve the effect of lower production cost, better effect and strong market competitiveness

Inactive Publication Date: 2016-08-03
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (2) The fermentation cycle is long and the degree of mechanization is low
Compared with the existing bacterial cellulose mask products on the market, there is no significant difference in microstructure, tensile strength, water retention characteristics, etc., but the production cost of nanocellulose gel film is greatly reduced, so that the end product is The price can be greatly reduced to meet the requirements of mass consumption

Method used

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  • Preparation method and application of nanoscale bacterial cellulose membrane
  • Preparation method and application of nanoscale bacterial cellulose membrane
  • Preparation method and application of nanoscale bacterial cellulose membrane

Examples

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preparation example Construction

[0042] Preparation of seed solution:

[0043] a. The formula of nutrients required for seed cultivation is as follows:

[0044] In terms of mass ratio (w / v), glucose 5-20%, peptone 0.5-2%, yeast extract 0.5-2%, citric acid 0.01-0.1%, Na 2 HPO 4 0.2~0.5%, KH 2 PO 4 0.1~0.2%, MgSO 4 0.025~0.05%, pH4.8~6.8, sterilized at 121℃ for 20min.

[0045] b. Seed activation method is as follows:

[0046] Pick a single colony on the plate and inoculate it in a fresh medium, add 0.01% cellulase, and culture on a shaker at 180 rpm at 30°C for 48 hours. Centrifuge at 5000rpm for 10min to obtain bacterial cell sedimentation, wash with sterile normal saline water 3 times to remove cellulase in the system, and finally dissolve the cleaned bacterial cells with the same volume of sterile water for use.

Embodiment 1

[0047] Embodiment 1: Synthesize bacterial cellulose with pullulan polysaccharide fermentation waste water as raw material

[0048] 1) Collect the pullulan fermentation broth, add 2 times the volume of ethanol to precipitate the polysaccharide in the fermentation broth, centrifuge at 5000rpm for 10min to obtain a mixture of fermentation broth and ethanol. The above mixture was transferred to a rotary evaporator to remove ethanol. The temperature of the water bath in the rotary evaporator was 40°C. An appropriate amount of distilled water was added to the alcohol-free fermentation broth obtained by rotary evaporation to make up for the water lost due to rotary evaporation.

[0049] 2) Use a biosensor analyzer to detect the sugar content in the fermentation medium, and adjust the sugar content of the fermentation broth to 2%. If it is lower than 2% (w / v), you need to add an appropriate amount of sucrose to ensure the normal growth of the bacteria; if it is higher than 2%, you nee...

Embodiment 2

[0055] Example 2: Synthesizing bacterial cellulose with pullulan polysaccharide fermentation waste cells as raw material

[0056] Pullulan polysaccharide is a polysaccharide obtained by fermentation of Aureobasidium pullulans. Aureobasidium pullulans, also known as Pullulan yeast, has its own cell wall, and the cells need to be fully broken to dissolve the nutrients. Example The method of ultrasonic crushing is used for wall breaking treatment.

[0057] 1) Collect the fermentation waste bacterial liquid, and centrifuge to obtain bacterial sediment. Add an appropriate amount of distilled water, mix well, and prepare a 10-20% bacterial suspension. As for the ultrasonic breaker, the cells are physically crushed, the crushing power is 400w, the crushing time is 30s, and the interval is 5s. Repeat 20 cycles. For foam, it is necessary to add an appropriate amount of defoamer to improve the crushing effect. The bacterial suspension obtained after ultrasonic crushing was placed in a...

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Abstract

The invention discloses a preparation method and application of a nanoscale bacterial cellulose membrane, and belongs to the technical field of comprehensive utilization of fermentation byproducts. The method comprises the following steps of: with pretreated waste bacteria solution or waste bacteria as fermentation raw materials, adjusting the content of nutritional ingredients in a fermentation medium, and inoculating 5-20% activated gluconacetobacter xylinus for static fermentation to obtain the nanoscale bacterial cellulose membrane. Proper treatment is carried out on the obtained bacterial cellulose membrane; a mask nutrient solution is added for low-temperature packaging, so that the beauty mask with a high added value is obtained; the mask is better in fitness and moisture retentiveness than an ordinary non-woven mask; meanwhile, the production cost is greatly reduced; the preparation cost of the mask material can be reduced by at least 99%; the overall production cost is reduced by at least 70%; and the preparation method has great market competitiveness.

Description

technical field [0001] The invention relates to a preparation method and application of a nanoscale bacterial cellulose membrane, belonging to the technical field of comprehensive utilization of fermentation by-products. Background technique [0002] Cellulose is the most abundant natural biomacromolecule substance on the earth. It is a polymer composed of glucose molecules connected by beta-1,4 glycosidic bonds. Its main source is trees, cotton and other plants. It is the main source of plant photosynthesis. product. Currently, cellulose from trees and cotton is the main raw material in the paper, textile, construction and chemical industries. Hundreds of millions of tons of cellulose are synthesized by plants every year on the earth, but the synthesis of cellulose is not unique to plants. Some bacteria, such as Acetobacter xylinum, Rhizobium, Agrobacterium and Pseudomonas, etc. High-purity nanoscale cellulose, also known as bacterial cellulose (BC), can be synthesized mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61K8/73A61K8/02A61Q19/00C12R1/02
CPCA61K8/0212A61K8/731A61Q19/00C12P19/04
Inventor 咸漠赵宏伟王纪明张海波
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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