Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, applied in the application of sewage treatment, heterotrophic nitrification-aerobic denitrification in the field of the temperature-resistant strain HAD-2 of M.

Pending Publication Date: 2016-08-17
CHINA UNIV OF PETROLEUM (EAST CHINA) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on strains with strong heterotrophic nitrification-aerobic denitrification performance and flocculant production at the same time, and strains

Method used

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  • Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof
  • Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof
  • Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Separation and purification of Pseudomonas mendoza strain HAD-2 of heterotrophic nitrification-aerobic denitrification

[0028]The samples were screened and separated from the activated sludge in Dongying Kenli Petrochemical Wastewater Treatment Plant, which was operating stably.

[0029] Medium formula: denitrification medium (g / L): sodium succinate 4.7, KNO 3 1.5, KH 2 PO 4 1.5, NaHPO 4 7.9, Mg 2 SO 4 ··7H 2 O 0.5, trace element solution 1mL; pH 7.5. Trace element solution (g / L): EDTA 50.0, ZnSO 4 2.2, CaCl 2 5.5g / L, MnCl 2 4H 2 O 5.06, FeSO 4 ·7H 2 O 5.0, (NH 4 ) 6 Mo 7 o 24 4H 2 O 1.1, CuSO 4 ·5H 2 O 1.57, CoCl 2 ·6H 2 O 1.61. Bromophenol blue (BTB) screening medium (g / L): L-asparagine 1.0, KNO 3 1.0, CH 3 COONa 8.5, KH 2 PO4 1.0, FeCl 2 ·6H 2 O0.05, CaCl 2 2H2O 0.2, MgSO 4 ·7H 2 O 1.0, bromophenol blue (BTB) solution (1% BTB and 99% ethanol) 5 mL; pH 7.0-7.3. Heterotrophic nitrification medium (g / L): with NH 4 Cl ins...

Embodiment 2

[0031] Embodiment two: identification of bacterial strain HAD-2

[0032] Strain morphology: observe the colony morphology of a single colony of the purified strain HAD-2, perform Gram staining, and observe with a transmission electron microscope. The bacterial strains were fixed with 2.5% glutaraldehyde, fully rinsed with buffer solution, dropped on the copper grid, stained with phosphotungstic acid, and observed by transmission electron microscope under high vacuum conditions. see results Attached picture 1 .

[0033] Physiological and biochemical experiments: According to the "Berger Bacteria Identification Handbook" and "Common Bacteria Identification Handbook" for physiological and biochemical identification, see the results surface 1 . The growth was measured at different temperatures, and it was found that the strain could grow at 15-45°C. It was a high-temperature-resistant strain and grew rapidly at 45°C.

[0034] surface 1 Physiological and biochemical char...

Embodiment 3

[0039] Embodiment three, the preparation of bacterial strain HAD-2 bacterial suspension

[0040] Inoculate a single HAD-2 colony into LB medium, culture at 35°C for 12-16 hours, and centrifuge to remove the supernatant. 0.9% sterilized physiological saline was used to resuspend the bacterial pellet to obtain the HAD-2 bacterial suspension. The LB medium contains (g / L): 10g peptone, 5g yeast extract, 10g sodium chloride, adjust the pH to 7.0, and sterilize at 121°C for 20 minutes.

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Abstract

The invention discloses a Pseudomonas mendocina temperature resisting strain HAD-2 being able to carrying out heterotrophic nitrification and aerobic denitrification and having flocculation ability. The strain is from activated sludge in the petrochemical sewage treatment plant, and is preserved in China General Microbiological Culture Collection Center on Jan. 20, 2016 with the preservation number of CGMCC 1.15625. The invention belongs to the technical field of environmental protection, and aims at solving the synchronous removal problem of ammonia nitrogen, nitrate nitrogen and total nitrogen in the sewage treatment process. The strain can realize simultaneous implementation of heterotrophic nitrification and aerobic denitrification under aerobic conditions to rapidly remove a heterotrophic nitrification system, an aerobic denitrification system, and ammonia nitrogen, nitrate nitrogen and total nitrogen in simulated sewage. Floc is produced by using LB, fermentation medium or nitrogen-containing medium fermentation. The strain has a wide application range, and has the advantage in treatment of high-temperature slight-alkaline sewage.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and specifically relates to a flocculation-producing heterotrophic nitrification-aerobic denitrification Mendosalmonas temperature-resistant strain HAD-2 and its application in sewage treatment. Background technique [0002] Biological nitrogen removal technology has been widely used in sewage treatment. Traditional biological denitrification methods need to be achieved through the coupling of microbial aerobic nitrification and anaerobic denitrification. Nitrifying bacteria and denitrifying bacteria have different requirements for oxygen and different environmental conditions. Therefore, in the engineering practice of sewage treatment, it is necessary to set up nitrification tanks and denitrification tanks, and the process is relatively complicated. Heterotrophic Nitrification-Aerobic Denitrification (HN-AD) is a new type of biological denitrification technology that can perfo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C02F3/02C02F3/34C12R1/38
CPCC02F3/02C02F3/34C12P1/04C12N1/205C12R2001/38Y02W10/10
Inventor 李静张璐王琳慧陈效儒郗丽君肖梓军
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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