Citrobacter freundii resisting yolk antibody, preparation method therefor and application of Citrobacter freundii resisting yolk antibody

Abstract

The invention discloses a Citrobacter freundii resisting yolk antibody, a preparation method therefor, a potency detection method for the Citrobacter freundii resisting yolk antibody and an application of the Citrobacter freundii resisting yolk antibody. The Citrobacter freundii resisting yolk antibody is characterized by being obtained through immunizing laying hens by adopting Citrobacter freundii as an antigen, collecting high-immunity eggs, and separating and purifying immune egg yolk. The preparation method comprises the steps: preparing the Citrobacter freundii antigen, and carrying out deactivating; carrying out fundamental immunization once and 3 to 4 times of booster immnunization on the laying hens, and collecting the eggs; and finally, carrying out centrifugal separation, salting-out and dialysis, thereby obtaining the product. The potency of the Citrobacter freundii resisting yolk antibody is detected by adopting indirect enzyme-linked immunosorbent assay, and the Citrobacter freundii resisting yolk antibody can be applied to the prevention and treatment of diseases induced by the Citrobacter freundii and can also be applied to the development of Citrobacter freundii detection kits and immune reaction and diagnosis in immunology. The Citrobacter freundii resisting yolk antibody has the advantages that the yolk antibody is relatively high in purity and has a relatively high inhibiting effect on the Citrobacter freundii, meanwhile, high-immunity yolk is cheap and convenient to obtain, low in cost and convenient in large-batch production, and the preparation method is simple and easy.

Description

technical field [0001] The invention relates to the field of preparation technology and application of biological products, in particular to an anti-Citrobacter freundii egg yolk antibody and its preparation method and application. Background technique [0002] Citrobacter freundii (Citrobacter freundii) is an opportunistic pathogen that is widely distributed in nature and is a normal flora in the intestinal tract of humans and animals. Like coliform bacteria, it can be regarded as a hygienic indicator of fecal pollution. Under certain conditions, Citrobacter freundii is a "human-accumulated" comorbid pathogen. When the body's resistance decreases, it can cause diarrhea, urinary tract infection, wound infection, arthritis and sepsis, etc.; Citrobacter can also exist in food and cause food poisoning; at the same time, Citrobacter freundii is also a widespread aquatic animal pathogen, which can cause diseases to both seawater and freshwater animals. Huge loss. According to r...

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Problems solved by technology

At present, yolk antibody has been widely concerned and applied in animal husbandry, but the research and development in the aquaculture industry is still in the basic stage. At present, the patents applied for publication and authorization in aquaculture include anti-prawn virus disease, anti-Cryptosporidium bayeri parahaemolyticus, anti-Vibrio anguillarum and anti-Aeromonas hydrophila yolk antibodies, but there is no relevant research report on anti-Citrobacter freundii yolk antibodies and their preparation methods and applications

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