Method of industrial production for trehalose by way of microbial fermentation

Abstract

A method of industrial production for trehalose by way of microbial fermentation comprises the steps of fermentation of trehalose hydrolase and trehalose synthase, starch liquefaction, catalytic reaction, glycosylation, decolorization and filtration, ion exchange, concentration, crystallization, centrifugation, drying, and membrane separation. The method of industrial production for trehalose by way of microbial fermentation has the following advantages: 1, starch is used as the raw material in the method to produce the trehalose through enzymatic catalysis, allowing mass and cheap industrial production of the trehalose; 2, high activity and low dosage of enzymes cause reduction of the production cost; 3, a centrifuged trehalose mother liquor is subjected to membrane separation such that the content of the trehalose is increased, and blending crystallization may be implemented to further increase the yield of the trehalose; 4, an innovative trehalose crystallization process causes the content of the trehalose to be above 50%, the content of glucose to be below 20%, and the content of the one-time crystallization product of the trehalose to be above 98.5%; 5, compared with a process of transforming the trehalose by using maltose, the method has great advantages, such as higher yield and lower production cost.

Description

technical field [0001] The invention belongs to the technical field of starch sugar production, and in particular relates to a method for the industrial production of trehalose by a microbial fermentation method. Background technique [0002] In November 2000, the Food and Agriculture Organization of the United Nations and the World Health Organization (FAO / WHO) Joint Expert Committee on Food Additives (JECFA) confirmed the function of trehalose as a tissue forming agent, stabilizer, moisturizer, and sweetener. The allowable intake is not limited; in March 2005, the Ministry of Health of my country approved trehalose as a new energy food. The production of trehalose has become a popular development project, and its production method has attracted widespread attention from domestic and foreign starch sugar manufacturers. It is reported that there are many production methods, which can be produced industrially, and there are few low-cost production methods. [0003] Trehalose...

AI Technical Summary

Problems solved by technology

[0004] The general preparation methods of trehalose reported at present (microbial extraction, microbial fermentation, enzymatic production, genetic recombination) are only small-scale experiments or theories, which cannot be industrialized, and the cost is very high

There is also an industrialized production method using maltose conversion, but the yield is low and the production cost is high

Images