Method of industrial production for trehalose by way of microbial fermentation

A microbial fermentation method and trehalose technology, applied in the field of starch sugar production, can solve the problems of unsuitable industrial production, high production cost, high cost, etc., and achieve the effects of low production cost, increased yield, and high enzyme activity

Active Publication Date: 2016-09-07
湖南金代科技发展有限公司
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Problems solved by technology

[0004] The general preparation methods of trehalose reported at present (microbial extraction, microbial fermentation, enzymatic production, genetic recombination) are only small-scale experiments or theories, which cannot be industrialized, and the cost is very high
There is also an industrialized production method using maltose conversion, but the yield is low and the production cost is high

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  • Method of industrial production for trehalose by way of microbial fermentation

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Embodiment 1

[0024] Weigh 1200kg of corn starch, add water to stir and adjust the pH value to 5.68, the concentration of the starch slurry is 14Be, the temperature of the first injection is 106°C, keep it warm for 40 minutes, check that the iodine color reaction is qualified, and the temperature of the second injection is 135°C; the liquefied liquid is 4.06M³, the concentration is 23.6 Bx, liquefied DE value 9.83%; cool down to 46°C, add pullulanase 800ml, add trehalose generating enzyme Y enzyme 28kg (enzyme fermentation broth), trehalose hydrolase Z enzyme 8kg (enzyme fermentation broth), and catalyze the reaction for 30 hours Raise the temperature to 61°C, adjust the pH to 4.4 with hydrochloric acid, add 400ml of glucoamylase, and conduct the saccharification reaction for 24 hours. 1365us / cm, volume 3.97M³ decolorized clear liquid; through cation and anion exchange, the conductivity is reduced to 21us / cm, pH5.8; concentrated to a concentration of 72.2%, volume 1.03M³, trehalose content 7...

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Abstract

A method of industrial production for trehalose by way of microbial fermentation comprises the steps of fermentation of trehalose hydrolase and trehalose synthase, starch liquefaction, catalytic reaction, glycosylation, decolorization and filtration, ion exchange, concentration, crystallization, centrifugation, drying, and membrane separation. The method of industrial production for trehalose by way of microbial fermentation has the following advantages: 1, starch is used as the raw material in the method to produce the trehalose through enzymatic catalysis, allowing mass and cheap industrial production of the trehalose; 2, high activity and low dosage of enzymes cause reduction of the production cost; 3, a centrifuged trehalose mother liquor is subjected to membrane separation such that the content of the trehalose is increased, and blending crystallization may be implemented to further increase the yield of the trehalose; 4, an innovative trehalose crystallization process causes the content of the trehalose to be above 50%, the content of glucose to be below 20%, and the content of the one-time crystallization product of the trehalose to be above 98.5%; 5, compared with a process of transforming the trehalose by using maltose, the method has great advantages, such as higher yield and lower production cost.

Description

technical field [0001] The invention belongs to the technical field of starch sugar production, and in particular relates to a method for the industrial production of trehalose by a microbial fermentation method. Background technique [0002] In November 2000, the Food and Agriculture Organization of the United Nations and the World Health Organization (FAO / WHO) Joint Expert Committee on Food Additives (JECFA) confirmed the function of trehalose as a tissue forming agent, stabilizer, moisturizer, and sweetener. The allowable intake is not limited; in March 2005, the Ministry of Health of my country approved trehalose as a new energy food. The production of trehalose has become a popular development project, and its production method has attracted widespread attention from domestic and foreign starch sugar manufacturers. It is reported that there are many production methods, which can be produced industrially, and there are few low-cost production methods. [0003] Trehalose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/16C12P19/14C12P19/12C12R1/19
CPCC12P19/12C12P19/14C12P19/16
Inventor 谢艳萍崔楠张国军李坚陈凯
Owner 湖南金代科技发展有限公司
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