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Immobilized microbial agent and preparation method and application thereof

A technology of bacterial agent and bacterial liquid, which is applied in the field of immobilized bacterial agent and its preparation, can solve the problems of ineffective repair of heavy oil spill pollution, easy predation by protozoa, and fast dilution of seawater, etc., to resist the competition of indigenous microorganisms Effect, fast adsorption speed, good growth effect

Active Publication Date: 2016-09-14
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above results give full affirmation to the prospect of immobilized bacterial agents for the restoration of intertidal oil spill pollution. However, these two immobilized bacterial agents are prepared by embedding with a variety of materials as carriers. The disadvantages of complex preparation process, high preparation cost, low mechanical strength of the preparation, and poor mass transfer performance limit the practical application of this type of product in the oil spill polluted intertidal zone environment
[0007] In addition, traditional free bacterial agents often have problems such as fast dilution of seawater, slow start-up speed, disadvantages in competition with indigenous bacteria, easy predation by protozoa, and sensitivity to environmental conditions during the restoration of coastline oil spill pollution, which cannot be effectively repaired Heavy oil spill pollution, especially in the intertidal zone

Method used

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  • Immobilized microbial agent and preparation method and application thereof
  • Immobilized microbial agent and preparation method and application thereof
  • Immobilized microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] This embodiment provides a screening method for Dietzia cercidiphylli strain HJZX-2, which is obtained by screening from heavy oil polluted intertidal zone with heavy crude oil as a single carbon source, and the screening process is as follows:

[0072] Take 2g of heavy oil-contaminated soil in 100mL of sterilized R2A medium, and enrich and culture it on a shaking table at 25°C-30°C, 140rpm, and dark for 5 days;

[0073] After two consecutive enrichment treatments, take the culture medium after the second transfer according to 10, 10 2 、10 3 、10 4 、10 5 and 10 6 Doubling serial dilution, and take 10 3 、10 4 、10 5 The two-fold dilutions were respectively applied to solid seawater medium containing 1g / L heavy oil, and cultured upside down in a 28°C incubator for 5d-7d;

[0074]According to the colony morphology of different microorganisms on the plate, use the inoculation loop to pick them out and streak them to the solid R2A medium plate, continue to invert the cu...

Embodiment 2

[0081] The present embodiment provides a kind of preparation method of modified zeolite, it comprises the following steps:

[0082] (1) High temperature modification

[0083] Natural zeolite (such as figure 2 Shown in A) burning for 2 hours to obtain high-temperature modified zeolite;

[0084] (2) acid modification

[0085] Take 10g of high-temperature modified zeolite and place it in 100mL of HNO with a concentration of 0.02mol / L 3 Soak in the aqueous solution for 24 hours, during which the glass rod is used for intermittent stirring; after the modification is completed, the modified zeolite is washed with deionized water for several times until it is neutral, and then placed in a constant temperature drying oven at 105 ° C to dry spare;

[0086] (3) Organic modification

[0087] Take 8g of zeolite modified by high temperature and acid modification, put it in 100mL aqueous solution of cetyltrimethylammonium bromide with a concentration of 12mol / L, react on a shaking tab...

Embodiment 3

[0089] This embodiment provides a kind of preparation method of immobilized bacterial agent, and its technological process is as follows figure 1 As shown, the method includes the following steps:

[0090] 1) Determination of immobilization conditions

[0091] The immobilization conditions of the bacterial agents were investigated through a single factor experiment, and the experimental design is shown in Table 1.

[0092] Table 1 Single factor design table of adsorption and fixation conditions

[0093]

[0094] Table 2 Plackeet-Burman experimental design and results

[0095] serial number

X1

X2

X3

X4

X5

X6

X7

Crude oil removal rate / %

1

1

1

-1

1

1

-1

1

48.82

2

1

-1

1

1

-1

1

-1

42.52

3

-1

-1

-1

1

1

1

-1

41.98

4

1

1

-1

1

-1

-1

-1

47.94

5

-1

-1

-1

-1

-1

-1

-1

42.97

6

1

-1

1

-...

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Abstract

The invention provides an immobilized microbial agent and a preparation method and application thereof. The preparation method comprises the following steps: inoculating Dietzia cercidiphylli strain HJZX-2 into a R2A medium to carry out activated culture so as to obtain a bacteria solution; inoculating the above bacteria solution into an immobilized medium, adding modified zeolite, placing the immobilized medium into a shaker, and culturing at predetermined immobilization temperature for immobilization time so as to obtain a liquid immobilized microbial agent. The technical scheme of the invention is simple and convenient to operate, preparation cost is low, and reaction conditions are mild. The product has high microbial activity and cell size, and degradation efficiency of petroleum hydrocarbon can reach 40.36%.

Description

technical field [0001] The invention relates to an immobilized bacterial agent and its preparation method and application, and belongs to the technical field of bioremediation of intertidal zones polluted by petroleum and petroleum products. Background technique [0002] In recent years, with the increasing intensity of exploration and development of offshore oil and gas resources and the rapid development of the shipping industry and coastal economy, marine oil spills and pollution accidents have occurred frequently, and oil and its products have become the second largest after cyanobacteria and red tides Big ocean pollution source. After the oil spill accident, although physical and chemical treatment can remove most of the surface oil spill, the residual petroleum hydrocarbon pollutants remain in the coastline environment for a long time, causing huge and lasting damage to the marine ecological environment and human health. [0003] At present, bioremediation technology ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N1/20C02F3/34C02F101/32C12R1/01
CPCC02F3/34C02F2101/32C12N11/14C12N1/205C12R2001/01
Inventor 宋佳宇阎光绪代小丽刘思敏王凌匀
Owner PETROCHINA CO LTD
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