Emulsion containing Heitacai polysaccharide
A technology of black cabbage and polysaccharide, which is applied in the field of emulsion containing black cabbage polysaccharide, can solve the problems of no antiseptic measures, no verification of compound effect, and inability to explain the effect, etc., achieve good economic benefits, simple preparation method, skin Mild and non-irritating effect
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Embodiment 6
[0067] Embodiment 6 multiple skin irritation tests
[0068] 1. Materials and methods:
[0069] (1) Preparation method of the test substance: the sample examples 1-5 and comparative examples 1-2 were directly tested respectively.
[0070] (2) Tested animals and feeding environment: 21 ordinary white New Zealand rabbits, divided into seven groups on average, 3 rabbits in each group, weighing 2.2-2.7kg, the temperature of the experimental animal breeding room was 19-26°C, and the relative humidity was 40 -70%.
[0071] (3) Test method: 24 hours before the test, the hair on both sides of the animal's back and spine was shaved, and the shaved range was about 3cm×3cm. Use distilled water as a control. Apply once a day for 7 consecutive days. From the next day, remove the hair before applying it every day, wash it with warm water, observe the skin reaction after 1 hour, and carry out the skin irritation reaction score according to the relevant regulations in the "Hygienic Standar...
Embodiment 7
[0079] Example 7 Determination of antioxidant activity
[0080] The preparation concentration is 1×10 -4 mol / L DPPH·dehydrated ethanol solution. The black tassel polysaccharide was formulated with 80% ethanol to prepare sample solutions to be tested with concentrations of 0.01, 0.04, 0.08, 0.10, 0.12, 0.16, 0.20, 0.24, and 0.26, respectively. Take 5mL sample solution and DPPH solution respectively, mix them evenly, place them in the dark for 30min, adjust to zero with 80% ethanol, and measure the absorbance Ai at 517BITI; in the same way, take 5mL80% ethanol solvent and sample solution and mix with each other evenly, avoiding Measure the absorbance Aj after standing in the light for 30min; add 5mL DPPH·solution to 5mL80% ethanol solvent and mix well, place in the dark for 30min, measure the absorbance Ac. Finally, the free radical scavenging rate is calculated according to the following formula: IP%=[1-(Ai-Aj) / Ac]×100, where Ai is the absorbance value after the sample intera...
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