Chitosan-sericin composite biological scaffold as well as preparation method and application thereof

A technology of sericin and bio-scaffold, which is applied in the field of medical biocomposite materials, can solve the problems of wasting natural resources, achieve good biocompatibility, and prevent tissue sticking

Active Publication Date: 2016-10-26
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, due to the lack of understanding of sericin and the limitations of research, serici

Method used

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  • Chitosan-sericin composite biological scaffold as well as preparation method and application thereof
  • Chitosan-sericin composite biological scaffold as well as preparation method and application thereof
  • Chitosan-sericin composite biological scaffold as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Preparation of chitosan-sericin composite bioscaffold

[0069] 1. Selection of silkworm cocoons:

[0070] The silkworm cocoons of silkworm silk fibroin-deficient mutant varieties were selected (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the silkworm silk fibroin-deficient mutant varieties were preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the preservation number (or product number) 185Nd-s, 140Nd-s, 139Nd-s As the raw material, the main chemical composition is: sericin.

[0071] 2. Choice of chitosan:

[0072] Chitosan was purchased from Sigma with a purity greater than 75%.

[0073] 3. Extraction and separation of sericin

[0074] 1) Weigh 1 g of silkworm cocoons of silk fibroin-deficient mutant varieties and cut them into 1 mm 2 Left and right fragments were placed in a clean beaker. Th...

Embodiment 2

[0090] Example 2 Performance analysis of chitosan-sericin composite bioscaffold

[0091] The chitosan-sericin composite bioscaffold prepared in embodiment 1 is carried out following performance analysis, specifically:

[0092] 1. Macro structure

[0093] Using the selected mold, the resulting chitosan-sericin composite scaffold was prepared into a sheet-like structure with a diameter of 1 cm and a height of 2-3 mm, such as figure 2 shown.

[0094] 2. Microstructure.

[0095] The chitosan-sericin composite bioscaffold was observed under a scanning electron microscope (ULTRA PLUS-43-13, Zeiss, Germany). Such as image 3 As shown, the SEM images of the cross-sections of five chitosan-sericin composite bioscaffolds with different ratios, the scale is 500 microns, and the interior of the scaffold is a multi-void structure, CS100 / SS0, CS80 / SS20, CS50 / SS50, CS20 / SS80 The average pore diameters of the five composite scaffolds, CS0 / SS100, are 157.69 μm, 130.55 μm, 146.27 μm, 136....

Embodiment 3

[0109]Example 3 Evaluation of Composite Biological Scaffold Supporting Nerve Cell Survival and Proliferation Ability

[0110] Use the aseptic chitosan solution prepared in Example 1 and the sericin aqueous solution to directly add in the cell well plate according to CS100 / SS0, CS80 / SS20, CS50 / SS50, CS20 / SS80, CS0 / SS100 five kinds of ratios, each Add genipin aqueous solution in various proportions, the amount added is 330 uL of genipin aqueous solution with a concentration of 0.01 g / mL per 1 mL of mixed solution, at 37 o C for 8 hours, followed by freezing at -80°C for 4 hours, and then freeze-drying in a vacuum freeze dryer to obtain a freeze-dried chitosan-sericin composite bioscaffold; Mouse Schwann cells (RSC96) were suspended, blown up, and seeded on cell culture plates covered with freeze-dried scaffolds. The medium used for cell culture was DMEM (Dulbecco's Modified Eagle's Medium) medium, 5% fetal bovine serum was added to the medium, and the cells were placed in a cel...

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Abstract

The invention provides a chitosan-sericin composite biological scaffold as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving chitosan by taking an acetic acid solution as a solvent to obtain a chitosan solution; preparing a sericin aqueous solution from the cocoon of bombyx mori of the fibroin-deleted mutant variety; uniformly mixing the chitosan solution and the sericin aqueous solution and pouring into a mould; adding a crosslinking agent for moulding to obtain a chitosan-sericin composite hydrogel; and freeze-drying the chitosan-sericin hydrogel to obtain the chitosan-sericin composite biological scaffold. The invention also provides a composite biological scaffold carrying a nerve growth factor NGF for peripheral nerve entrapment treatment. The composite biological scaffold provided by the invention has good biocompatibility and relatively high porosity and degradability, and the degradation product also has a neurotropic effect; the composite scaffold also has antibacterial and anti-adhesion functions and the like; and compared with the traditional way of relieving sciatic nerve compression simply by operation, the composite biological scaffold provided by the invention can effectively promote the repairing of peripheral nerve entrapment injury.

Description

technical field [0001] The invention relates to the field of medical biological composite materials, in particular to a chitosan-sericin composite biological scaffold, a composite biological scaffold loaded with nerve growth factor, and a preparation method and application thereof. Background technique [0002] Peripheral nerve entrapment is a common disease in clinical surgery, with a prevalence rate as high as 3.8% in the population. It mainly includes carpal tunnel syndrome, cubital tunnel syndrome, radial tunnel syndrome, and common peroneal nerve entrapment syndrome. Nerve entrapment can occur anywhere in the body, most commonly in bony fibrous tubes near joints, especially in hypertrophic tendon arches, aberrant ligaments, and space-occupying lesions (ganglion cysts, lipomas, hematomas, or tumors) in the course of nerves etc.) can squeeze the peripheral nerve. Traditional peripheral nerve entrapment treatment methods include surgical treatment and conservative treatme...

Claims

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Application Information

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IPC IPC(8): A61L27/48A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/48A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/252A61L2300/414A61L2300/602A61L2430/32C08L5/08C08L89/00
Inventor 王琳王征杨文张磊谢洪建王健李晓麟
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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