Preparation method of microbial particles for producing xanthan gum

A technology of microorganisms and xanthan gum, which is applied in the field of preparation of composite microbial particles, can solve problems such as low output, low viscosity of aqueous solution, and impact on product quality, and achieves the effect of low cost and simple preparation steps

Inactive Publication Date: 2016-10-26
郭舒洋
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: aiming at the low yield of Xanthan gum produced by Xanthan gum, and the low viscosity of the aqueous solution of the produced xanthan gum, which affects the quality of the product, a method of first using Calcium carbonate rich in limestone, through high-temperature calcination and hydrochloric acid soaking to expand its gaps, and then by loading the beneficial oxygen-promoting substances in seaweed, and mixing them with nutrients to attract and adsorb microorganisms to make a composite microbial particle carrier, and then wild rapeseed Xanthomonas is inoculated into the prepared medium, induced by sodium nitrite and nitrosoguanidine, supplemented by ultraviolet radiation for mutagenesis screening, to obtain high-yielding strains, and finally adsorb them to the composite microbial particle carrier, then Process for obtaining microbial particles for the production of xanthan gum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] First take limestone, clean its surface with water, put it into a high-temperature furnace, calcinate at 220°C for 50 minutes, cool to 90°C, take out the activated limestone, soak it in a hydrochloric acid solution with a mass fraction of 15%, and let it stand After 35 minutes, the pretreated limestone is taken out, and the pretreated limestone is washed with distilled water until the pH of the washing solution is neutral, then put into a pulverizer for crushing, and passed through a 200-mesh sieve; Put sodium sarcosinate into the container, then add distilled water twice the mass of sieved particles into the container, stir at 200r / min for 16min, then filter, dry the filtrate, and take fresh Put seaweed and distilled water into a mill, grind into a slurry, filter, and collect the filtrate; then soak the above-mentioned dried filtrate in the above-mentioned filtrate, and place it in a microwave instrument, at 28°C Under microwave vibration for 3 days, then filter, colle...

example 2

[0022]First take limestone, clean its surface with water, put it into a high-temperature furnace, calcinate at 180°C for 40 minutes, cool to 80°C, take out the activated limestone, soak it in a hydrochloric acid solution with a mass fraction of 15%, and statically After standing for 25 minutes, the pretreated limestone is taken out, and the pretreated limestone is washed with distilled water until the pH of the washing solution is neutral, then put into a pulverizer for crushing, and passed through a 200-mesh sieve; Sodium acyl sarcosinate is put into the container, and then distilled water with 1 times the mass of the sieved particles is added to the container, stirred at 200r / min for 12min and then filtered, and the filtered matter is dried, and the solid-liquid ratio is 1:2. Put fresh seaweed and distilled water into a mill, grind into a slurry, filter, and collect the filtrate; then soak the dried filtrate in the above-mentioned filtrate, and place it in a microwave instrum...

example 3

[0025] First take limestone, clean its surface with water, then put it into a high-temperature furnace, calcinate at 200°C for 45 minutes, cool to 85°C, take out the activated limestone, soak it in a hydrochloric acid solution with a mass fraction of 15%, and statically After standing for 30 minutes, the pretreated limestone is taken out, and the pretreated limestone is washed with distilled water until the pH of the washing solution is neutral, then put into a pulverizer for crushing, and passed through a 200 mesh sieve; Sodium acyl sarcosinate is put into a container, and then distilled water with 1 times the mass of the sieved particles is added to the container, stirred at 200r / min for 15min, and then filtered, and the filtered matter is dried, and the solid-to-liquid ratio is 1:2. Put fresh seaweed and distilled water into a mill, grind into a slurry, filter, and collect the filtrate; then soak the dried filtrate in the above filtrate, and place it in a microwave instrumen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
viscosityaaaaaaaaaa
quality scoreaaaaaaaaaa
quality scoreaaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparation method of composite microbial particles for producing xanthan gum, belonging to the technical field of xanthan gum preparation. The method comprises the following steps of: activating, pretreating and grinding limestone respectively; mixing the obtained particles with sodium lauroyl sarcosine and filtering; soaking the filtered object in a filtering medium prepared from gloiopeltis; performing microwave oscillation; mixing the filtered object with the substances such as agar and tryptone to obtain a composite microbial particle carrier; inoculating the prepared culture medium with sarson xanthomonas, and performing constant-temperature culture; leaching the culture medium after culture by using sterile water; submerging the composite microbial particle carrier in the leacheate; and inoculating the leacheate to obtain the microbial particles for producing xanthan gum. In the invention, the prepared microbial particles can be used for mass production of xanthan gum, and the yield of the xanthan gum reaches 5.35-6.12g / 100mL; the viscosity coefficient of the produced xanthan gum is high and reaches 50.2-60.5mPa.s.

Description

technical field [0001] The invention relates to a method for preparing composite microbial particles for producing xanthan gum, belonging to the technical field of xanthan gum preparation. Background technique [0002] Xanthan gum, also known as xanthan gum and yellow single-cell polysaccharide, is a widely used microbial exopolysaccharide produced by fermentation of Xanthomonas campestris with carbohydrates such as corn starch as the main raw material. Xanthan gum is soluble in cold water and hot water, and forms a high-viscosity solution after fully hydrating, which has an efficient thickening effect and unique rheological properties; good heat resistance, salt resistance, and acid and alkali resistance; Various other hydrocolloids, such as starch, locust bean gum, guar gum, etc., have synergistic effects; in addition, xanthan gum also has good freezing / thawing resistance. As a high-efficiency thickener and stabilizer, xanthan gum is widely used in the food industry such ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/02C12P19/06C12R1/64
CPCC12N11/14C12N11/02C12N11/10C12P19/06
Inventor 郭舒洋薛培龙宋奇
Owner 郭舒洋
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap