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Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice

A technology of inorganic anions and organic acids, applied in the field of chemistry, can solve the problems of inability to meet the requirements of simultaneous detection, failure to meet detection requirements, and low detection efficiency, and achieve reduced detection costs, less matrix interference components, and high detection sensitivity Effect

Active Publication Date: 2016-10-26
日照海关综合技术服务中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using different detection standards, different pretreatment methods, and different detection instruments (HPLC, IC) will consume a lot of manpower and material resources, and the detection work efficiency is low, which cannot meet the growing detection needs, and can be retrieved in the current database. The only thing to do is to simultaneously detect a certain type of components (single organic acid or pure inorganic anion), or the number of components to be detected at the same time is small (for example, only more than 10 components are analyzed at the same time), which cannot meet the requirements of multiple components in the fruit juice matrix. Requirements for Simultaneous Detection of Multiple Ions in Class Components

Method used

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  • Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice
  • Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice
  • Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice

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Experimental program
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Effect test

Embodiment 1

[0040] Determination of chromatographic conditions

[0041] 1. Selection of chromatographic column: The experimental separation effects of AS11, AS11-HC, and AS19 three chromatographic columns have been investigated successively. It is found that under the same conditions, the peak shape is the sharpest and the comprehensive separation effect is the best when the AS11-HC chromatographic column is used. This is because AS11-HC is a high-capacity chromatographic column, which has a good retention effect on organic acids and inorganic anions, and is very suitable for simultaneous detection of multiple components. Therefore, the AS11-HC chromatographic column was selected for experimental determination.

[0042] 2. Selection of flow rate: The experimental separation under the flow rate conditions of 0.5mL / min, 1.0mL / min, 1.5mL / min, and 2.0mL / min has been investigated successively. The chromatographic separation effect at 0.5mL / min is better than that at 1.0mL / min When the time de...

Embodiment 2

[0047] The accuracy of the assay method

[0048] 1. Detection limit and calibration curve

[0049] Prepare standard working solutions of 18 kinds of organic acids and 8 kinds of inorganic anions with different concentrations, and test them on the machine, and obtain the chromatographic separation diagrams of 26 kinds of component standard solutions. See the attached drawings for details. figure 1 . The detection limit (S / N=3) of the components to be tested was calculated by the chromatographic peak signal-to-noise ratio. Within the concentration range of 0.02mg / L to 10.0mg / L, the detection and linearity of standard working solutions at 9 different concentration points (0.02, 0.04, 0.10, 0.20, 0.50, 1.0, 2.0, 5.0, 10.0mg / L) were carried out The fitting and correlation coefficients were all greater than 0.995, indicating that under the experimental conditions, the linearity was good when multiple components were detected simultaneously. See Table 2 for details, where Y is the...

Embodiment 3

[0074] Actual sample measurement

[0075] Six common fruit juice drinks (apple juice, grape juice, orange juice, peach juice, pear juice, and mixed fruit juice) were selected to detect the contents of organic acids and inorganic anions in actual samples.

[0076] Before testing, use KOH solution with a concentration of 4.0mol / L, adjust the pH value to neutral (6.5-8.0), take 10mL sample and put it into a 50mL centrifuge tube, centrifuge at 10000r / min at high speed for 15min, and weigh the supernatant Sample 1.00g, dilute to 100mL volumetric flask with ultrapure water. Shake well, let it stand for 10 minutes, take 10.0mL solution, pass through a 0.22μm aqueous phase filter membrane, discard the first 3mL, collect the latter clear liquid, and wait for detection.

[0077] The resulting supernatant was tested on the computer according to the ion chromatography conditions and the multi-stage gradient elution procedure, and the results were calculated.

[0078] The specific testin...

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Abstract

The invention discloses a method for simultaneously detecting 26 organic acids and inorganic negative ions in juice and a juice manufacturing method. According to the method, a juice sample is preprocessed; multilevel-inhibition conductance ion chromatography is utilize to simultaneously detect 18 organic acids (quinic acid, lactic acid, propionic acid, formic acid, butyric acid, pyruvic acid, pentanoic acid, acetic acid, malonic acid, malic acid, citric acid, glutaric acid, maleic acid, fumaric acid, benzoic acid, cis-aconitic acid, trans-aconitic acid, and oxalic acid) and 8 inorganic negative ions (F<->, Cl<->, NO3<->, NO2<->, ClO2<->,BrO3<->, PO4<3->, and CrO4<2->) in a juice; the sample pretreatment is simple, rapid and accurate; the technical problem that multiple components in juice cannot be accurately analyzed is solved, organic acids and inorganic negative ions in juice can be accurately measured, a technical support is provided for judging nutritional value, freshness degree, and true / false of juice, and the components of juice can be detected by the method.

Description

technical field [0001] The invention relates to a detection method in the field of chemistry, in particular to a method for simultaneous detection of 26 kinds of organic acids and inorganic anions in fruit juice. Background technique [0002] Organic acids are a class of organic compounds with acidic characteristics, widely present in the fruits, stems and leaves of plants. The content of organic acids in fruits, vegetables, juices and other foods we eat daily will directly affect the taste and flavor of foods. Organic acids can represent the quality of products and identify the freshness of foods. Inorganic anions are an indispensable part of fruits, vegetables and their products. Excessive content of certain anions will endanger human health, and even accumulate in the body and transform into carcinogens. It can be seen that the importance of the determination of inorganic anions is also beyond doubt. [0003] The common organic acids and inorganic anions in fruit juices ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 宋卫得袁晓鹰高尧华苏征惠希东吕宁刘冰王霞
Owner 日照海关综合技术服务中心
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