Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice

A technology of inorganic anions and organic acids, applied in the field of chemistry, can solve the problems of inability to meet the requirements of simultaneous detection, failure to meet detection requirements, and low detection efficiency, and achieve reduced detection costs, less matrix interference components, and high detection sensitivity Effect

Active Publication Date: 2016-10-26
日照海关综合技术服务中心
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  • Abstract
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Problems solved by technology

Using different detection standards, different pretreatment methods, and different detection instruments (HPLC, IC) will consume a lot of manpower and material resources, and the detection work efficiency is low, which cannot meet the growing detection needs, and can be retrieved in the current database. The only thing to do is to simultaneously detect a certain typ

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  • Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice
  • Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice
  • Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice

Examples

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Example Embodiment

[0039] Example one

[0040] Determination of chromatographic conditions

[0041] 1. Chromatographic column selection: The experimental separation effects of AS11, AS11-HC, and AS19 columns were successively investigated, and it was found that under the same conditions, the AS11-HC column has the sharpest peak shape and the best comprehensive separation effect. This is because AS11-HC is a high-capacity chromatographic column, which has good retention of organic acids and inorganic anions, and is very suitable for simultaneous detection of multiple components. Therefore, the AS11-HC column was selected for experimental determination.

[0042] 2. Selection of flow rate: the experimental separation under flow rate conditions of 0.5mL / min, 1.0mL / min, 1.5mL / min, and 2.0mL / min have been investigated successively, and the chromatographic separation effect at 0.5mL / min is better than 1.0mL / min Although the resolution is slightly larger than that at 1.0 mL / min, the total peak time becomes l...

Example Embodiment

[0046] Example two

[0047] The accuracy of the measurement method

[0048] 1. Detection limit and calibration curve

[0049] Prepare standard working solutions of 18 kinds of organic acids and 8 kinds of inorganic anions with different concentrations respectively, and test on the computer to obtain chromatographic separation diagrams of 26 kinds of standard solutions, see the attached drawings in the instructions for details figure 1 . The detection limit (S / N=3) of the component to be tested is calculated by the chromatographic peak signal-to-noise ratio. In the concentration range of 0.02mg / L to 10.0mg / L, 9 different concentration points (0.02, 0.04, 0.10, 0.20, 0.50, 1.0, 2.0, 5.0, 10.0mg / L) standard working solution detection and linearity The fitting and correlation coefficients are both greater than 0.995, indicating that under the experimental conditions, the linearity is good when multiple components are detected simultaneously. See Table 2 for details, where Y is the pea...

Example Embodiment

[0073] Example three

[0074] Actual sample measurement

[0075] Six common fruit juice drinks (apple juice, grape juice, orange juice, peach juice, pear juice, mixed juice) were selected, and the contents of organic acids and inorganic anions in actual samples were detected.

[0076] Before the test, use a KOH solution with a concentration of 4.0mol / L, adjust the pH to neutral (6.5-8.0), take 10mL of the sample into a 50mL centrifuge tube, centrifuge at a high speed at 10000r / min for 15min, and weigh the supernatant The sample is 1.00g, dilute to a 100mL volumetric flask with ultrapure water. Shake well, let it stand for 10 minutes, take 10.0 mL of the solution, pass through a 0.22 μm water phase filter membrane, discard the first 3 mL, collect the clear liquid afterwards, and wait for testing.

[0077] The resulting supernatant was tested on the machine according to ion chromatography conditions and multi-stage gradient elution procedures, and the results were calculated.

[0078] T...

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Abstract

The invention discloses a method for simultaneously detecting 26 organic acids and inorganic negative ions in juice and a juice manufacturing method. According to the method, a juice sample is preprocessed; multilevel-inhibition conductance ion chromatography is utilize to simultaneously detect 18 organic acids (quinic acid, lactic acid, propionic acid, formic acid, butyric acid, pyruvic acid, pentanoic acid, acetic acid, malonic acid, malic acid, citric acid, glutaric acid, maleic acid, fumaric acid, benzoic acid, cis-aconitic acid, trans-aconitic acid, and oxalic acid) and 8 inorganic negative ions (F<->, Cl<->, NO3<->, NO2<->, ClO2<->,BrO3<->, PO4<3->, and CrO4<2->) in a juice; the sample pretreatment is simple, rapid and accurate; the technical problem that multiple components in juice cannot be accurately analyzed is solved, organic acids and inorganic negative ions in juice can be accurately measured, a technical support is provided for judging nutritional value, freshness degree, and true/false of juice, and the components of juice can be detected by the method.

Description

technical field [0001] The invention relates to a detection method in the field of chemistry, in particular to a method for simultaneous detection of 26 kinds of organic acids and inorganic anions in fruit juice. Background technique [0002] Organic acids are a class of organic compounds with acidic characteristics, widely present in the fruits, stems and leaves of plants. The content of organic acids in fruits, vegetables, juices and other foods we eat daily will directly affect the taste and flavor of foods. Organic acids can represent the quality of products and identify the freshness of foods. Inorganic anions are an indispensable part of fruits, vegetables and their products. Excessive content of certain anions will endanger human health, and even accumulate in the body and transform into carcinogens. It can be seen that the importance of the determination of inorganic anions is also beyond doubt. [0003] The common organic acids and inorganic anions in fruit juices ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 宋卫得袁晓鹰高尧华苏征惠希东吕宁刘冰王霞
Owner 日照海关综合技术服务中心
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