Medical tissue closing glue composition, medical tissue closing glue and preparation method and application thereof

A technology of sealant and composition, which is applied in the field of biomedicine, can solve the problems of functional effects and use position limitations, and achieve good biocompatibility, high anti-rupture strength, and pain relief effects

Active Publication Date: 2016-11-09
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, GEFOAM's absorbable gelatin (Pharmacia & Upjohn, Kalamazoo, MI), Johnson & Johnson (Shanghai) Medical Equipment Co., Ltd.'s Bioseal and Surgiflo, and fibrin sealants, etc.; although natural biomaterials have the advantages of low cost, reliable sources, and easy access , as an implantable medical material has a long history of use and safety evaluation, but its functional effect and use position have always been highly limited

Method used

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  • Medical tissue closing glue composition, medical tissue closing glue and preparation method and application thereof
  • Medical tissue closing glue composition, medical tissue closing glue and preparation method and application thereof
  • Medical tissue closing glue composition, medical tissue closing glue and preparation method and application thereof

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preparation example Construction

[0037] The present invention provides a medical tissue sealant composition, a medical tissue sealant and its preparation method and application. The medical tissue sealant composition of the present invention mainly includes a first component containing a nucleophile and a second component containing an electrophile. Two components, the nucleophilic reagent includes a natural substance containing an amino group and / or a first modification product that is modified with a sulfhydryl group on the natural substance containing an amino group, and the natural substance containing an amino group and the first modification product are independently have more than 10 active groups, wherein the active groups are amino groups and mercapto groups; the electrophilic reagents include the second modified with more than 10 vinyl groups on chitosan and / or polylysine modified product. Preferably, in the first component, the position where the thiol group is modified is the position of the amino...

Embodiment 1

[0094]

[0095] 1) Weighing 15mmol (2.418g) of chitosan with a degree of deacetylation of 87.5%, 20mmol (1.73mL) of mercaptopropionic acid, 20mmol (3.8350g) of soluble carbodiimide, 20mmol (2.3010g) of N-hydroxysuccinimide g), placed in 100 mL of purified water at a temperature of 5° C., and stirred for 8 hours.

[0096] 2) Dialyze the reaction mixture obtained in step 1) in 5 L of normal saline for 12 hours to remove unreacted monomers and small molecular substances, and replace the normal saline every 2 hours.

[0097] 3) Put the mixture obtained by dialysis in step 2) into a freeze dryer for freeze-drying to obtain chitosan modified with sulfhydryl groups, which is the first component.

[0098]

[0099] 1) Weigh 15mmol (2.418g) of chitosan with a degree of deacetylation of 87.5%, 20mmol (1.4mL) of acrylic acid, 20mmol (3.8350g) of soluble carbodiimide, and 20mmol (2.3010g) of N-hydroxysuccinimide, Place in 100 mL of purified water at 10°C, and stir to react for 12 hour...

Embodiment 2

[0106]

[0107] 1) Weigh 0.4887 g of poly-L-lysine hydrochloride with a weight-average molecular weight between 3600 and 4300 and a molar percentage of primary amino groups of 35%, wherein the molar mass of primary amino groups is 1.5 mmol, 20mmol (1.73mL) of mercaptopropionic acid, 20mmol (3.8350g) of soluble carbodiimide, and 20mmol (2.3010g) of N-succinimide were placed in 100mL purified water at a temperature of 5°C, and stirred for 8 hours.

[0108] 2) Dialyze the reaction mixture obtained in step 1) in 5 L of normal saline for 12 hours to remove unreacted monomers and small molecular substances, and replace the normal saline every 2 hours.

[0109] 3) The mixture obtained by dialysis in step 2) is placed in a lyophilizer for lyophilization to obtain poly-L-lysine modified with sulfhydryl groups, which is the first component.

[0110]

[0111] 1) Weigh 15mmol (2.418g) of chitosan with a degree of deacetylation of 87.5%, 20mmol (1.4mL) of acrylic acid, 20mmol (3.8350g)...

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Abstract

The invention provides a medical tissue closing glue composition, medical tissue closing glue and a preparation method and application thereof. The medical tissue closing glue composition comprises a first component and a second component, wherein the first component comprises an amino containing natural substance and/or a first modified product obtained by modifying the amino containing natural substance with sulfydryl groups, and the amino containing natural substance and the first modified product respectively have ten or more active groups which are amino groups and sulfydryl groups; the second component comprises a second modified product obtained by modifying chitosan and/or polylysine with ten or more vinyl groups. The medical tissue closing glue has high rupture strength, a low swelling ratio and good adhesiveness, tissue wound surfaces can be quickly closed, the effect of closing tissue is effectively achieved, interstitial fluid or blood or cerebrospinal fluid is prevented from leaking, the pain of a patient can be relieved, and more danger is avoided.

Description

technical field [0001] The invention relates to a medical tissue sealing glue composition, a medical tissue sealing glue and a preparation method and application thereof, belonging to the technical field of biomedicine. Background technique [0002] Medical tissue sealant is mainly used in medicine to seal and stop bleeding sites during or after surgery, physically fill defect tissues, prevent tissue viscera from sticking, and prevent interstitial fluid leakage. The main materials of medical tissue sealant can be divided into two categories according to the composition: one is artificially synthesized hydrogel-based materials, such as polyethylene glycol, acrylamide, etc.; the other is natural biological materials, such as gelatin, collagen, shell, etc. Polysaccharides, sodium alginate, cellulose, etc. [0003] Synthetic hydrogel materials are divided into two categories: degradable and non-degradable. Among them, the degradable materials are mostly polyethylene glycol, po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/04A61L24/00A61L27/20A61L27/18A61L27/58C08B37/08C08G69/48C08F251/00C08F220/06C08F283/04
CPCA61L24/0042A61L24/046A61L24/08A61L27/18A61L27/20A61L27/58A61L2400/04A61L2430/34C08B37/003C08F220/06C08F251/00C08F283/04C08G69/48C08L5/08C08L77/04
Inventor 马骋邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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