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Film-substrate micro-through-hole-array biochip and preparation method thereof

A technology of biochip and manufacturing method, which is applied in the field of biological and medical detection, can solve problems such as uneven aperture, single shape, and high price, and achieve the effects of good repeatability, flexible design, and low cost

Inactive Publication Date: 2016-11-16
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the micropore distribution of the nuclear pore membrane is random, many pores overlap, the shape is single, the pore size is uneven, and the whole set of filter equipment is expensive, reaching several thousand yuan

Method used

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  • Film-substrate micro-through-hole-array biochip and preparation method thereof
  • Film-substrate micro-through-hole-array biochip and preparation method thereof
  • Film-substrate micro-through-hole-array biochip and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0025] A kind of PI thin film base microvia array biochip involved in this embodiment is as follows: figure 1 As shown, the structure is as follows:

[0026] The thickness is 10 μm, which is a uniform and transparent PI film 1. The micro-through hole array 2 is distributed on the film 1. The micro-through hole is circular and has a full-through structure. The pore diameter is 8 μm, and the ratio of the pore diameter to the gap between the holes is 1: 1. The micro-via array 2 is arranged in a rectangular distribution. Its scanning electron microscope picture is as Figure 5 shown.

[0027] The flow chart of a method for manufacturing a PI film substrate micro-through-hole array biochip involved in this embodiment is as follows Figure 4 As shown, the steps are as follows:

[0028] (1) In a 500ml beaker, add 239.1g of dimethylacetamide, then add 2.3g of p-phenylenediamine, 24.3g of 4,4'-diaminodiphenyl ether, 21.8g of pyromellitic dianhydride, 12.6 g of pyromellitic dianhyd...

Embodiment 2

[0033] A kind of PMMA film substrate micro-through-hole array biochip involved in this embodiment is as follows: figure 2 As shown, the structure is as follows:

[0034] It is a uniform and transparent PMMA film 1 with a thickness of 5 μm. Micro-via arrays 2 are distributed on the film 1. The micro-vias are square and have a full-through structure. The diameter of the micro-vias is 4 μm. 1:5, the micro-via array 2 is arranged in a rectangular distribution.

[0035] A flow chart of a method for manufacturing a PMMA film substrate micro-through-hole array biochip involved in this embodiment is as follows Figure 4 As shown, the steps are as follows:

[0036] (1) Take 3g of PMMA particles with an electronic balance, put into a glass bottle filled with 30ml of glacial acetic acid, stir and let stand for 2 hours to obtain a PMMA solution. The PMMA solution was coated on a silicon wafer, the coating speed was 2000 rpm, baked at 150° C. for 20 minutes, thermally cured, and cooled...

Embodiment 3

[0041] A kind of PU film substrate micro-through-hole array biochip involved in this embodiment is as follows: image 3 As shown, the structure is as follows:

[0042] It is a uniform and transparent PU film 1 with a thickness of 60 μm. On the film 1 is distributed an array of micro-vias 2 . The micro-vias are regular hexagonal and full-through structures. :10, the micro-via array 2 is arranged in a regular hexagonal distribution.

[0043] The flow chart of a method for manufacturing a PU film substrate micro-through-hole array biochip involved in this embodiment is as follows Figure 4 As shown, the steps are as follows:

[0044] (1) Put 106g of acrylate and 110g of polyethylene glycol into a flask, heat it to 50°C, slowly add 196g of toluene diisocyanate into the flask while stirring, keep it warm for 2 hours, slowly drop to room temperature, add diluent The agent is mixed uniformly to obtain a polyurethane prepolymer. Add the polyurethane prepolymer, acrylate and benzoin ...

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Abstract

The invention discloses a film-substrate micro-through-hole-array biochip and a preparation method thereof. The film-substrate micro-through-hole-array biochip is mainly based on the property that cancer cells usually greater than normal cells and employs a series of through holes with diameters greater than the diameters of normal cells and less than the diameters of cancer cells for separation of cancer cells from normal cells by using a filtering and screening method. The film-substrate micro-through-hole-array biochip provided by the invention is a transparent film with uniform thickness, and micro-through-hole arrays are distributed on the film; and biochip is prepared by using a photoetching method. According to the invention, a transparent-film through-hole structure is employed for separation of cancer cells, which facilitates combination of conventional mature pathological analysis means and observation excited by an inverted fluorescence microscope after dyeing and prevents interference by backlight; moreover, the film is a high polymer material which is widely available and low in cost; the shapes and distribution of micro through holes are diversified; so the biochip has a higher degree of freedom in performance optimization and industrial application and promotion of the biochip are benefited.

Description

technical field [0001] The invention relates to the field of biological and medical detection, in particular to a membrane-based micro-hole array biological chip for separating and detecting circulating tumor cells and a manufacturing method thereof. Background technique [0002] With the continuous improvement of living standards, people pay more and more attention to life and health, and cancer is a major problem affecting people's life and health. When cancer is discovered, it is often too late. How to diagnose cancer as early as possible and treat it in an early stage can often save people's lives. Since more than 90% of cancer deaths are caused by metastasis, and circulating tumor cells are the direct source of tumor metastases, detection of circulating tumor cells from blood has attracted more and more attention. [0003] The amount of circulating tumor cells in peripheral blood is very small, and every 10ml of blood may only contain a few to several hundred circulati...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12N5/09C12Q1/02
CPCC12N5/0693G01N33/5017
Inventor 张之胜张为国王赟姣张东夏良平汤明杰史浩飞杜春雷
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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