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Finger print detection method of traditional Chinese medicine composition

A detection method and fingerprint technology, applied in the field of drug analysis, to achieve the effects of good system adaptability, strong specificity and strong repeatability

Active Publication Date: 2016-11-16
SHANGHAI KAIBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since traditional Chinese medicine and its preparations are multi-component complex systems, the evaluation of their quality should adopt detection methods that are suitable for it and can provide rich identification information, but the current methods of microscopic identification, physical and chemical identification and content determination are not enough. to solve this problem

Method used

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  • Finger print detection method of traditional Chinese medicine composition
  • Finger print detection method of traditional Chinese medicine composition
  • Finger print detection method of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of Chinese medicine composition soft capsule

[0042] Take 375g of cloves and 375g of cinnamon, add 8 times the amount of water, distill it over water for 8 hours, collect the volatile oil, refrigerate for 24 hours, remove the water, and make a total of 36ml of volatile oil.

[0043] Mix 36ml volatile oil with 364ml soybean oil and set aside. Take 150g of gelatin, 60g of glycerin, and 10g of monosyrup, add 150g of deionized water and mix well, heat to 60-70℃, keep for 0.5 hours, add 0.15g of methyl paraben and 0.075g of ethyl paraben, stir , Evacuate and keep at 70°C for 20 minutes to make the bag material. Take the mixture of volatile oil and soybean oil, use the capsule material to make soft capsules by the compression method, inspect and sub-package to obtain 1000 soft capsules.

Embodiment 2

[0044] Example 2 Investigation of chromatographic conditions

[0045] 1 Instruments and reagents

[0046] Shimadzu Shimadzu GC-2010Plus gas chromatograph, FID detector; electronic balance; Mili-Q pure water instrument; KQ-500DE ultrasonic cleaning instrument.

[0047] Ethanol, methanol, ethyl acetate, n-hexane, and ether are analytically pure; cinnamaldehyde (98%, batch number: PS151228-05) and eugenol (98%, batch number: PS0262-0500) were purchased from Chengdu Pusi Biotech Co., Ltd. Co., Ltd., the Chinese medicine composition is a soft capsule prepared according to Example 1.

[0048] 2 Preparation of sample solution

[0049] The preparation method of the test solution: accurately weigh 0.8 g of the contents of the soft capsule and place it in a 50 mL volumetric flask. Dilute to the mark with n-hexane, ether, ethyl acetate, absolute ethanol, and methanol, and shake well. The results showed that the ethyl acetate group was clear and transparent and dissolved well, while the other gro...

Embodiment 3

[0062] Example 3 Methodological investigation

[0063] 1 Preparation of sample solution

[0064] Preparation of test solution: accurately weigh 0.8 g of the contents of the soft capsule, put it in a 50 mL measuring flask, add ethyl acetate to dissolve, dilute to the mark, filter through a microporous membrane (0.45 μm), and seal for later use.

[0065] Preparation of reference substance solution: accurately weigh out an appropriate amount of cinnamaldehyde reference substance, dilute with ethyl acetate, and dilute to a 100mL volumetric flask to prepare a reference substance solution with a concentration of about 3.0mg / mL, microporous filter membrane (0.45μm) Filter, seal, store at 4°C for later use.

[0066] 2 Chromatographic conditions

[0067] Chromatographic conditions: Column: 6% cyanopropyl phenyl / 94% dimethyl polysiloxane as stationary phase -624 capillary column (30m×0.25mm, 1.4um); carrier gas is nitrogen (99.99%); SPL1 is 250°C; FID1 is 270°C, initial column temperature is 12...

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Abstract

The invention discloses a gas chromatograph finger print detection method of a traditional Chinese medicine composition taking clove and cassia barks as bulk drugs and belongs to the field of pharmaceutical analysis. Conditions of gas chromatograph comprise: a capillary column is selected as a chromatographic column; a hydrogen flame ionization detector is adopted; the carrier gas is nitrogen with a split ratio being 5-20:1; the column flow is 0.5-1 ml / min; the detector temperature (FID1) is 250-270 DEG C, the sampling port temperature (SPL1) is 230-250 DEG C, the initial column temperature is 100-140 DEG C, and the temperature can reach 200-240 DEG C after temperature rise. The gas chromatograph finger print detection method is excellent in system suitability, good in specificity, stability and repeatability, and high in precision degree, can conform to requirements for constructing a finger print, and can be applied to quality control of a traditional Chinese medicine composition or a preparation thereof. The method is high in reliability and easy and convenient to operate.

Description

Technical field [0001] The invention discloses a fingerprint detection method of a traditional Chinese medicine composition, and specifically discloses a fingerprint detection method of a traditional Chinese medicine composition prepared from cloves and cinnamon as raw materials, and belongs to the field of drug analysis. Background technique [0002] The invention relates to a traditional Chinese medicine preparation prepared by combining two medicines of clove and cinnamon according to a certain ratio and extracting the volatile oil thereof. The main components are eugenol and cinnamaldehyde. The preparation has the effects of warming and dispelling cold pathogens, promoting qi and relieving pain, and is suitable for abdominal pain in irritable bowel syndrome, aggravated cold, diarrhea, constipation, abdominal distension and pain, belching, chills, urge to defecate or swelling, and feeling of bloating after defecation. Pain reduction, pale tongue, white tongue coating, stringy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025
Inventor 张振华陈军李小雪陈琳刘绍勇
Owner SHANGHAI KAIBAO PHARMA