Autolysis process method for producing high-activity material yeast autolysate and yeast product prepared by same

A yeast autolysate and process method technology, applied in the field of yeast autolysis, can solve problems such as low yeast autolysis efficiency, and achieve the effects of reducing control difficulty, increasing nucleotide content, and reducing drying costs

Pending Publication Date: 2016-12-07
ICELL SUSTAINABLE SHANGHAI NUTRITION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0034] The purpose of the present invention is to solve the defects of existing yeast autolysis efficiency, and provide a high-efficiency, active ingredient-rich production method using endogenous enzyme-rich specific single-cell proteins and yeast mixed autolysis

Method used

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  • Autolysis process method for producing high-activity material yeast autolysate and yeast product prepared by same
  • Autolysis process method for producing high-activity material yeast autolysate and yeast product prepared by same
  • Autolysis process method for producing high-activity material yeast autolysate and yeast product prepared by same

Examples

Experimental program
Comparison scheme
Effect test

manufacture example

[0066] In this example, the complex flora rich in endogenous enzymes cultivated in real time from the organic wastewater of a beer factory is used as the raw material for specific single-cell proteins. After the complex flora is cultivated, most of the extracellular water is removed through the process of sedimentation and centrifugation. After precipitation, the flora contains water. The rate is 90% to 98%, and the water content is reduced to 80% to 85% after centrifugal dehydration. The protein content (dry basis) of the complex flora is higher than 50%, and it is transported to the autolysis equipment by a meterable screw pump.

[0067] The yeast is commercially available common dried non-autolyzed beer yeast with a protein content of 50%.

[0068] Add the prepared composite flora and yeast at a ratio of 50% / 50% of dry matter, and the addition of the ratio is realized by the frequency of the screw conveying pump and the motor of conveying Jiaolong.

[0069] In addition to t...

Embodiment 2

[0078] This example is an example of raw material selection, autolysis production process and products. In this example, the complex flora rich in endogenous enzymes cultivated in real time in the organic wastewater of a beer factory is used as raw materials, and Tween 80 is used as the enzyme production during the cultivation process. Accelerator.

[0079] After the complex flora is cultivated, most of the extracellular water is removed through the process of sedimentation and centrifugation, and the protein content is higher than 50%, which is transported to the autolysis equipment through a meterable screw pump.

[0080] The yeast is dehydrated fresh yeast obtained by press filtration from the beer factory. It has not been dried, and the protein (dry basis) is higher than 50%. The prepared composite flora and yeast are added in a dry matter ratio of 50% / 50%. The way to increase the proportion is realized by the motor frequency of the screw conveying pump and conveying Jiaol...

Embodiment 3

[0089] Embodiment 3 is an embodiment of the determination of small peptides and free amino acids in the sample prepared by the present invention

[0090] Principles and methods

[0091] The content of small peptides and free amino acids is used to verify the degree of hydrolysis of yeast protein after autolysis, and to compare and analyze the degree of hydrolysis and nutritional value before and after autolysis.

[0092] Amino acids were eluted using a reversed-phase C18 chromatographic column and a buffered salt system of acetonitrile and sodium acetate. The small peptide content of the sample is equal to the total amino acid content of the supernatant after perchloric acid treatment minus the free amino acid content in the supernatant.

[0093] Reagent and Solution Preparation

[0094] 12mol / L hydrochloric acid (containing 0.2% phenol): Weigh 1000mg of phenol into 500ml concentrated hydrochloric acid with a concentration of 38% 12mol / l, and mix well.

[0095] 1.5mol / L per...

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Abstract

The invention discloses an autolysis process method for producing high-activity material yeast autolysate. Specific single-cell protein not treated through sterilization and keeping original endogenous enzyme activity and yeast are mixed and autolyzed according to the dry biomass of 2-8 to 8-2, then dried and sterilized, autolysis is performed at a low temperature period and a high temperature period, in the low temperature period, the temperature ranges is kept at 45 to 60 DEG C for 20 to 60 minutes, and in the high temperature period, the temperature is kept at 75 to 95 DEG C for 30 to 120 minutes. According to the autolysis process method provided by the invention, the specific single-cell protein containing rich endogenous enzyme is innovatively taken as an exogenous enzyme directly applied to yeast autolysis, the activity of the exogenous enzyme is higher than that of industrial enzyme, and since the autolysis temperature is raised, the problem of pollution caused by over low temperature can be solved, and product quality is improved.

Description

technical field [0001] The invention relates to the technical field of yeast autolysis. Background technique [0002] Yeast is widely used in bread fermentation, beer fermentation, animal feed and other fields, and there are many types, including bread yeast, beer yeast, molasses yeast, etc. Many existing food ingredients, such as molasses, cornstarch, corn syrup, and sugar beets, can be fermented to produce large amounts of yeast. [0003] Yeast is rich in nutrients, containing protein, carbohydrates, 8 kinds of amino acids and 14 kinds of minerals necessary for the human body, and contains almost all kinds of vitamins, nucleic acids, glutathione and other nutrients. Yeast can release internal nutrients after wall breaking, enzymolysis, autolysis and other processes, so that it has a strong fragrance, and a large number of nutrients such as small peptides and flavor amino acids, free nucleotides, vitamins, etc. can also be released from the inside , so that it can be dire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06A23K10/18A23K20/147A23K20/153A23L33/14A23L33/195A23L33/13
CPCC12N1/06A23V2002/00C12N1/063A23V2250/546A23V2250/76
Inventor 曾靖婷
Owner ICELL SUSTAINABLE SHANGHAI NUTRITION CO LTD
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