Method for preparing butanediamine

A technology of butanediamine and succinonitrile, applied in the field of biochemistry, can solve problems such as inability to synthesize butanediamine, and achieve the effects of great social significance and economic value, high efficiency and yield, and simple process

Active Publication Date: 2016-12-14
南京荔枝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved in the present invention is to provide a method for preparing butanediamine, so as to solve the problem in the prior art that the C4 platform compound of succinic acid cannot be synthesized into butanediamine

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] This example illustrates the process of producing ammonium succinate by fermentation of Escherichia coli BEW308.

[0033] The culture medium formula described in the present embodiment:

[0034] Plate medium formula is: citric acid 3g / L, Na 2 HPO 4 12H 2 O 4g / L, KH 2 PO 4 8g / L, MgSO 4 ·7H 2 O1g / L, CaCl 2 2H 2 O 10.0mg / L, ZnSO 4 ·7H 2 O 0.5mg / L, CuCl 2 2H 2 O 0.25mg / L, MnSO 4 ·H 2 O2.5mg / L, CoCl 2 ·6H 2 O 1.75mg / L, H 3 BO 3 0.12mg / L, Al 2 (SO4) 3 1.77mg / L, Na 2 MoO 4 2H 2 O0.5mg / L, ferric citrate 16.1mg / L, agar 20g / L, glucose 10g / L, with (NH 4 ) 2 HPO 4 Provide ammonium ions and make NH 4 + The concentration is 0.5mol / L, and the addition amount is 32g / L;

[0035] The formula of the seed medium is: peptone 10g / L, yeast powder 5g / L, NaCl 5g / L;

[0036] The formula of fermentation medium is: citric acid 3g / L, with (NH 4 ) 2 HPO 4 10g / L, Na 2 HPO 4 12H 2 O 4g / L, KH 2 PO 4 8g / L, MgSO 4 ·7H 2 O 1g / L, CaCl 2 2H 2 O 10.0mg / L, ZnSO 4...

Embodiment 2

[0040] This example illustrates the process of using Escherichia coli BEW308 to produce ammonium succinate and obtain a clarified supernatant after fermentation.

[0041] In order to obtain clarified supernatant in this embodiment, process ① disc centrifugation, ultrafiltration, and activated carbon column were established; process ② microfiltration, ultrafiltration, and activated carbon column two processes were established. The test results are as follows:

[0042]

[0043]

[0044] It can be seen from the test results that both processes can obtain better clarified ammonium succinate aqueous solution, but considering the actual operation process, the process ① has a smaller loss rate of the product, and the operation and operation costs are lower.

Embodiment 3

[0046] This embodiment illustrates the process of clarifying ammonium succinate aqueous solution to prepare butanediamine. In order to obtain butanediamine, the following process is established:

[0047] Using vacuum distillation, control the pressure of the concentration tank to -0.09Mpa, and the temperature at 60°C, and evaporate the supernatant to dryness to obtain crude crystals; transfer the crystals into a 500mL reactor, and control the temperature at 60°C under normal pressure. React at 190°C for 3 hours, the effluent is mainly water and part of ammonia gas, which can be directly connected to the waste water collection tank; connect the vacuum pump to control the vacuum degree of the reactor at 8-9kpa, control the temperature at 180°C for 2 hours, and collect the distillate, namely D Dinitrile; distillate is added to hydrogenation reactor, ethanol is solvent, and addition is 1.1 times (volume ratio) of succinonitrile, and Raney nickel is catalyst, and addition 5%, and po...

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Abstract

The invention discloses a method for preparing butanediamine. With ammonium succinate as a raw material, the butanediamine is obtained through a dehydration reaction and a hydrogenation reaction. Meanwhile, according to the method, a strain capable of tolerating high-concentration ammonium ions and producing succinic acid at a high yield can also be combined, the ammonium succinate is prepared through anaerobic fermentation, a clear aqueous solution of the ammonium succinate is obtained through a simple solid-liquid separation process and a simple decolorization process under an unpurified and unseparated condition, and ethylenediamine is prepared, so that a method for biologically preparing the ethylenediamine is provided. Compared with the prior art, the method provided by the invention has the advantages of simple process and extremely high efficiency and yield. Meanwhile, in the whole process, renewable biomass resources are taken as raw materials, and each process is relatively simple and can be implemented, so that the method has great social significance and great economic value.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for preparing butanediamine. Background technique [0002] Succinic acid, also known as succinic acid, is a common natural organic acid. As an intermediate product of the TCA cycle and one of the terminal reduction products of anaerobic metabolism, it plays a very important role in the process of biological metabolism. In recent years, with the increasing depletion of fossil resources and the increasingly serious environmental problems, the use of biological methods to prepare succinic acid has attracted much attention. The US Department of Energy has listed succinic acid as one of the 12 most valuable biorefinery products in the future . Biosynthesis of succinic acid is to use various microorganisms such as bacteria and fungi to produce succinic acid through microbial fermentation with glucose or other various hydrolyzates as carbon sources. Compared with chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C209/48C07C211/09C12P7/46C12R1/19
CPCC07C209/48C07C253/22C12P7/46C07C211/09C07C255/04
Inventor 马江锋罗继宏
Owner 南京荔枝生物科技有限公司
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