Gracilaria slag fermented active feed and preparation method thereof
A technology of Gracilaria and activity, which is applied in the field of fermented active feed of Gracilaria residue and its preparation, can solve the problems of large protein molecular weight, difficult direct and effective utilization of Gracilaria residue by livestock and poultry, and difficult degradation of crude cellulose and residual agar, etc. Achieve the effect of reducing emissions and improving growth performance
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[0013] The specific preparation method of the Gracilaria gracilis slag fermentation active feed provided by the invention mainly includes the following steps:
[0014] 1. The slants of Aspergillus oryzae, Bacillus subtilis LYN-1, high temperature resistant Saccharomyces cerevisiae and Lactobacillus acidophilus were expanded and cultivated respectively, transferred to the triangular flask for culture, and further enlarged, and the fermenter was used for liquid fermentation culture to prepare strains;
[0015] 2. Mix gracilaria dregs, corn flour, corn starch, soybean meal, bran, glucose, trace elements and water in a mixing tank, sterilize them, and inoculate after cooling to room temperature;
[0016] 3. The four microbial strains prepared are connected to the mixing tank according to a certain inoculation amount and mixed with the solid-state fermentation substrate, and a certain amount of water is added to control the humidity of the material at 45-55wt%;
[0017] 4. Transfer...
Embodiment 1
[0022] Species: Aspergillus oryzae, Bacillus subtilis LYN-1, thermostable Saccharomyces cerevisiae and Lactobacillus acidophilus. Aspergillus oryzae and high temperature Saccharomyces cerevisiae were screened and preserved in this laboratory. Bacillus subtilis LYN-1 was deposited on June 21, 2012 in the General Microbiology Center of China Microorganism Culture Collection Management Committee (CGMCC for short), and the registration number is: CGMCC No. 6265. Lactobacillus acidophilus originated from the China Industrial Microorganism Culture Collection Center (strain number: 6075), and the four strains are now preserved in the Department of Pharmacy, Fujian Institute of Health Vocational Technology.
[0023] Proceed as follows to prepare seeds separately:
[0024] 1. Preparation of Aspergillus oryzae seed solution: Take the slanted surface of the preserved Aspergillus oryzae eggplant bottle, rinse it with sterile water in a sterile room, and inoculate it into 30 500mL conical...
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