Ultra-small metal chalcogenide compound nano crystal and biological synthesis method and application thereof

A synthesis method and nanocrystal technology, applied in the direction of silver compounds, silver compounds, chemical instruments and methods, etc., can solve the problems of large mercury sulfide nanoparticles, not suitable for in vivo application, weakened absorption, etc., and achieve good dispersion, uniform size, Highly stable effect

Inactive Publication Date: 2017-01-11
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the Cu prepared by this method 2-x S is very unstable in the surrounding atmospheric environment and is easily oxidized by oxygen, resulting in a significant weakening of the absorption in the near infrared.
[0005] The invention patent with the publication number CN102249291A records a preparation method based on goat anti-human antibody as a soft template to control the synthesis of nanoparticles of mercury sulfide. It mainly uses the antibody as a template, and first adds mercury ions to form a composite of protein and mercury ions. Then add sulfide ions, the sulfide ions combine with the mercury ions on the protein molecules to form surface-modified mercuric sulfide nanoparticles of sheep anti-human antibody, but this technology still has some defects, for example, the mercuric sulfide nanoparticles are relatively large (average particle size greater than 10nm), and not suitable for in vivo application

Method used

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  • Ultra-small metal chalcogenide compound nano crystal and biological synthesis method and application thereof
  • Ultra-small metal chalcogenide compound nano crystal and biological synthesis method and application thereof
  • Ultra-small metal chalcogenide compound nano crystal and biological synthesis method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 uses bovine serum albumin (BSA) as a template to prepare ultra-small CuS nanocrystals, comprising the following steps:

[0064] 1. Take 0.05mol / L CuCl 2 Put 1.0mL of the solution, 80mg of bovine serum albumin (BSA, Mw=66,430KDa) and 24mL of 1 deionized water into a 100mL two-necked round-bottomed flask. Too large to prevent the protein from forming a lot of foam.

[0065] 2. Inject 25 ml of NaOH solution (PH=9.0) into the reaction system, and continue stirring at room temperature.

[0066] 3. After stirring for 5 minutes, add 7.5 uL of 85% hydrazine hydrate, and stir for 2 minutes, and the solution turns light yellow.

[0067] 4. Quickly inject 320mg / mL Na 2 100uL of S solution was heated up to 60°C, reacted at this temperature for 2h, and then cooled to room temperature to obtain a light green solution.

[0068] 5. Use a 100KDa ultrafiltration tube, set the speed of the centrifuge to 3000RPM, perform ultrafiltration concentration and washing to remove ...

Embodiment 2

[0069] Embodiment 2 uses bovine serum albumin (BSA) as a template to prepare ultra-small CdS nanocrystals, comprising the following steps:

[0070] 1. Take 0.05mol / L CdCl 2 Put 1.0mL of the solution, 80mg of bovine serum albumin (BSA, Mw=66,430KDa) and 24mL of deionized water into a 100mL two-neck round bottom flask, and stir it magnetically for 0.5h at room temperature to make it fully mixed. Too large to prevent the protein from forming a lot of foam.

[0071] 2. Inject 25 mL of NaOH solution (PH=9.0) into the reaction system, and continue stirring at room temperature.

[0072] 3. Quickly inject 100uL of Na with a concentration of 320mg / ml 2 S solution, heated up to 60°C, reacted at this temperature for 2h, then cooled to room temperature to obtain a yellow solution.

[0073] 4. Use a 100KDa ultrafiltration tube, set the speed of the centrifuge at 3000RPM, perform ultrafiltration, concentration and washing to remove free BSA protein molecules, and finally disperse the pro...

Embodiment 3

[0074] Embodiment 3 uses bovine serum albumin (BSA) as a template to prepare ultra-small size ZnS nanocrystals, comprising the following steps:

[0075] 1. Take 0.05mol / L ZnCl 2 Put 1.0mL of the solution, 80mg of bovine serum albumin (BSA, Mw=66,430KDa) and 24mL of deionized water into a 100mL two-neck round bottom flask, and stir it magnetically for 0.5h at room temperature to make it fully mixed. Too large to prevent the protein from forming a lot of foam.

[0076] 2. Quickly inject 100uL of Na with a concentration of 320mg / ml 2 S solution, heated up to 80°C, reacted at this temperature for 2h, then cooled to room temperature to obtain a colorless solution.

[0077] 3. Use a 100KDa ultrafiltration tube, set the speed of the centrifuge at 3000RPM, perform ultrafiltration, concentration and washing to remove free BSA protein molecules, and finally disperse the product into 4.0mL of deionized water for later use.

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Abstract

The invention discloses an ultra-small metal chalcogenide compound nano crystal and a biological synthesis method and application thereof. In one embodiment, the synthesis method is as follows: a protein with the isoelectric point PI lower than 9.0 and a metal salt are mixed with water evenly to form a mixed solution, the mixed solution is adjusted to alkaline, a reducing agent and a sulfur source are added for reaction to form the ultra-small metal chalcogenide compound nano crystal with the particle size less than 10nm. The method has the characteristics of mild reaction conditions, simple operation, direct use of particles without additional surface modification in biological photothermal therapy, and the like, the prepared ultra-small metal chalcogenide compound nano crystal is uniform in size, good in dispersion and high in stability, and the prepared ultra-small metal chalcogenide compound nano crystal has very good bio-compatibility without any surface modification, also has the photothermal effect, and is a great-application-prospect nano material used for photothermal therapy and biological fluorescence imaging.

Description

technical field [0001] The present invention relates to a kind of nanoparticle and its preparation method, especially a kind of ultra-small size, monodisperse, hydrophilic, biocompatible metal chalcogenide nanoparticle (nanocrystal) and its preparation method and application. Background technique [0002] Due to their unique optical properties, sulfide plays an important role in the research of biomedicine, especially antitumor medicine. For example: CuS, Ag 2 S is often used as a photothermal therapeutic agent for research because of its good photothermal effect in the near-infrared region, and CdS and ZnS and their heterojunctions have been used for the first time since 1998 by Chan, Nie and Bruchez et al. After the CdS quantum dots successfully labeled HeLa cells and 3T3 fibroblasts, they were deeply studied and developed as bioimaging probes. However, there are still many shortcomings in the above synthesis of sulfide nanocrystals, and the preparation of CuS nanocryst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G3/12C01G11/02C01G9/08C01G5/00C01G49/12C01G21/21C01B19/04A61K49/00A61K41/00A61P35/00B82Y30/00
Inventor 王新张倩胡慧珊
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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