Yeast culture and preparing method and application thereof

A technology of yeast culture and yeast, which is applied in the field of yeast culture and its preparation, can solve the problems of being unable to be popularized in a large scale, long production time, and large floor area, so as to achieve improved daily feed intake, good quality, The effect of low price

Inactive Publication Date: 2017-01-11
北京中农弘科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid fermentation process is simple, fast, and efficient to operate, but due to equipment, energy consumption and other reasons, there are problems that it cannot be widely promoted; the solid fermentation process avoids dehydration, collection and drying in the liquid fermentation process, reducing energy consumption. And reduce the discharge of waste liquid, but because the solid fermentation bacteria need to be cultivated in multiple stages, it is easy to be polluted by miscellaneous bacteria, the production time is long, and the problem of occupying a large area

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  • Yeast culture and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the preparation of yeast culture

[0029] 1. Activation of slant strains: inoculate the Saccharomyces cerevisiae with the preservation number of CGMCC No.2.399 frozen and preserved on the slant medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L, and agar 2%. Keep the temperature at 28°C and culture for 40 hours to obtain activated yeast strains.

[0030] 2. Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into liquid medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value is 5.6, shake at 28°C Bed culture, rotation speed 120r / min; culture 18h.

[0031] 3. Liquid aerobic fermentation: Inoculate the prepared seed liquid with 10% inoculum into a new liquid culture medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value 5.6 The expanded culture was carried out in the tank, the ventilation rate was controlled to be 5 L / min, the temperature was 28° C., and the fermentat...

Embodiment 2

[0038] Embodiment 2, the preparation of yeast culture

[0039] The difference from Example 1 is that the composition of the solid medium is 7 parts of straw, 5 parts of corn flour and 1 part of bran.

[0040] The carbon to nitrogen ratio of the solid medium was 4:1.

[0041] The cell wall-breaking enzyme is pectinase, purchased from Sigma Company in the United States; the enzyme activity of pectinase is 5.0 U / mg.

[0042] The enzyme activity of pectinase is defined as follows:

[0043] Under the condition of 50°C and pH value of 4.0, the amount of enzyme needed to decompose polygalacturonic acid to produce 1 μmol galacturonic acid in 1 minute is 1 enzyme activity unit.

[0044] After testing, the crude protein content of the product prepared in this example is 14.5%, the mannan content is 1.0%, the B-glucan content is 3.8%, and the ash content is 6.5%.

Embodiment 3

[0045] Embodiment 3, the preparation of yeast culture

[0046] This embodiment is the best implementation mode of this application.

[0047] 1. Activation of slant strains: inoculate the Saccharomyces cerevisiae with the preservation number of CGMCC No.2.399 frozen and preserved on the slant medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L, and agar 2%. Keep the temperature at 30°C and culture for 48 hours to obtain activated yeast strains.

[0048] 2. Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into liquid medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value is 6.5, and shake at a constant temperature of 30°C Bed culture, rotation speed 160r / min; culture 24h.

[0049] 3. Liquid aerobic fermentation: Inoculate the prepared seed liquid with 20% inoculation amount into a new liquid culture medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value 5.6 Expanded culture was carrie...

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Abstract

The invention discloses yeast culture and preparing method and application thereof. The method for preparing the yeast culture includes the following steps: after activating the brewer's yeast with slant strains, the barms acquired continues to inoculate for seed solution preparing, and implement liquid aerobic fermentation and solid anaerobic fermentation for the seed solution succesively, then make the acquired solid fermentation products into yeast culture through cell disruption, drying and smashing. The yeast culture supplied has a high content of crude protein and stable, and can reach about 15%; and the contents of mannan, Beta- glucan are respectively higher than 1.0% and 3.5%. It is a feed ingredient with high nutritive value, and can improve feed palatability, animal food consumption, and animal intestinal health and growth performance; the invention adopts the invention adopt liquid- solid combined fermentation process, to develop cheap yeast culture with favorable effect and lower price, and the process is simple, easy to operate, and the whole production process conditions are easy to control, no need of high temperature treatment, which may favorably store the nutrient substance in the yeast culture.

Description

technical field [0001] The invention relates to the technical field of animal breeding feed, in particular to a yeast culture and its preparation method and application. Background technique [0002] Since 2006, the Chinese government has also promulgated a series of specifications for the use of feed drug additives, which has limited the application of feed antibiotics in livestock breeding. Fully tapping my country's existing protein feed resources is the key to ensuring the continued stability of my country's feed industrial-grade breeding industry. The inevitable way of development, and the research of yeast culture has received more and more attention. [0003] Yeast culture is a micro-ecological product formed by yeast on a specific medium (such as corn, wheat bran, oats and other grains) after sufficient anaerobic fermentation under specific process conditions, mainly composed of extracellular metabolic products , the mutated medium after fermentation and a small amou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/865A23K10/12A23K10/37A23K10/30A23K10/38A23K20/10A23K50/15A23K50/30A23K50/60A23K50/10
CPCC12P1/02C12N1/185C12R2001/865Y02P60/87
Inventor 刘明
Owner 北京中农弘科生物技术有限公司
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