Device and method for continuous cultivation and production of lysine

A technology for producing lysine and lysine, which is applied in biochemical equipment and methods, biological material sampling methods, biochemical cleaning devices, etc., can solve the problems of contamination, burden, time-consuming and labor-intensive, etc.

Active Publication Date: 2017-02-22
SHOUGUANG GOLDEN FAR EAST MODIFIED STARCH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the fermentation, the acid adjustment tank is stored for a period of time, and the problem of bacterial contamination is prone to occur. The acid adjustment of the fermentation broth is adjusted in batches, and there is instability between batches, which will cause a burden for the subsequent extraction process.
Then enter the ceramic membrane for filtration, the clear liquid enters the continuous ion exchange resin, and the waste water from the ion exchange is directly discharged, which causes great pollution to the environment
Part of the lysine data needs to be detected during the fermentation and extraction process, such as the lysine concentration in the fermenter, the lysine content in the clear liquid after the fermentation broth passes through the membrane, the lysine content in the high-concentration wastewater after separation, The lysine content in the collected liquid after handing over, the lysine content in the collected liquid after concentration and other indicators, according to the routine detection, need to take samples and use the ninhydrin method for detection, which is not only time-consuming and laborious, but also lags behind in the control of continuous production parameters

Method used

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  • Device and method for continuous cultivation and production of lysine
  • Device and method for continuous cultivation and production of lysine
  • Device and method for continuous cultivation and production of lysine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] A method for continuously cultivating and producing lysine, comprising the steps of:

[0084] (1) Uninterrupted cultivation: After the first-level seed tank is cultivated to a certain bacterial concentration, it is transferred to the second-level seed tank for cultivation according to a certain inoculation amount, and the first-level seed tank is supplemented with a certain volume of medium for the first-level seed tank to continue the cultivation , according to the growth time of the first-level seed tank, the second-level seed tank inoculation is carried out after reaching the required bacterial concentration, and the continuous cycle is continuously cultivated; in this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.45, and the second-level seed tank is maintained. The OD of the bacteria in the seed tank is 0.9;

[0085] (2) Uninterrupted fermentation: Transfer the bacterial liquid obtained from the cultivation of the secondary seed...

Embodiment 2

[0094] A method for continuously cultivating and producing lysine, comprising the steps of:

[0095] (1) Uninterrupted cultivation: After the first-level seed tank is cultivated to a certain bacterial concentration, it is transferred to the second-level seed tank for cultivation according to a certain inoculation amount, and the first-level seed tank is supplemented with a certain volume of medium for the first-level seed tank to continue the cultivation , according to the growth time of the first-level seed tank, the second-level seed tank inoculation is carried out after reaching the required cell concentration, and the continuous circulation is cultivated successively; in this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.65, and the second-level seed tank is maintained. The OD of the bacteria in the seed tank is 1.1;

[0096] (2) Uninterrupted fermentation: Transfer the bacterial liquid obtained from the cultivation of the secondary see...

Embodiment 3

[0105] A method for continuously cultivating and producing lysine, comprising the steps of:

[0106] (1) Uninterrupted cultivation: After the first-level seed tank is cultivated to a certain bacterial concentration, it is transferred to the second-level seed tank for cultivation according to a certain inoculation amount, and the first-level seed tank is supplemented with a certain volume of medium for the first-level seed tank to continue the cultivation , according to the growth time of the first-level seed tank, the second-level seed tank inoculation is carried out after reaching the required cell concentration, and the continuous circulation is cultivated successively; in this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.5, and the second-level The OD of the bacteria in the seed tank is 1;

[0107] (2) Uninterrupted fermentation: Transfer the bacterial liquid obtained from the cultivation of the secondary seed tank to the first fermente...

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PUM

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Abstract

The invention discloses a device for continuous cultivation and production of lysine. The device comprises a primary seed tank, a secondary seed tank and a fermentation tank group, wherein the primary seed tank, the secondary seed tank and the fermentation tank group are sequentially connected in series; the fermentation tank group is composed of a No.1 fermentation tank, a No.2 fermentation tank, a No.3 fermentation tank and a No.4 fermentation tank which are serially connected in series; a discharged material mixer and a settling tank are sequentially connected to the endmost side of the fermentation tank group in series; and an iron-exchange resin system, an MVR (mechanical vapor recompression) concentrator, a continuous crystallizing system, a centrifugal system and a fluidized bed drying system are sequentially arranged at the downstream side of the settling tank. Meanwhile, the invention also discloses a method for continuous cultivation and production of the lysine. With the application of the device and the method provided by the invention, investment cost to the seed tanks and the fermentation tanks is reduced, a fermentation flow is shortened and lysine fermentation cost is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of amino acid production, in particular to a method and device for producing lysine by continuous culture. Background technique [0002] Lysine is the eight basic amino acids necessary for the human body (also known as essential amino acids, they are lysine, threonine, leucine, isoleucine, methionine, valine, phenylalanine and tryptophan )one. Lysine has a high solubility in water, and it is easy to absorb carbon dioxide in the air, so it is difficult to obtain its crystallization. Therefore, as a commodity it often appears in the form of monohydrochloride. Lysine has a wide range of uses and is mainly used in the following industries: food industry, preparation of nutritional enhancers, flavoring agents, and sweeteners; feed industry, preparation of nutritional enhancers; pharmaceutical industry, preparation of amino acid infusions, amino acid derivatives, and amino acid salts; Chemical industry, prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/00C12P13/08
CPCC12M23/58C12M27/02C12M29/06C12M33/04C12M47/10C12P13/08
Inventor 吴泽华李刚王志强孙敬善杨秋霞李玉玲罗明刚刘汉生孙振
Owner SHOUGUANG GOLDEN FAR EAST MODIFIED STARCH CO LTD
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