Device and method for continuous cultivation and production of lysine
A technology for producing lysine and lysine, which is applied in biochemical equipment and methods, biological material sampling methods, biochemical cleaning devices, etc., can solve the problems of contamination, burden, time-consuming and labor-intensive, etc.
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Embodiment 1
[0083] A method for continuously cultivating and producing lysine, comprising the steps of:
[0084] (1) Uninterrupted cultivation: After the first-level seed tank is cultivated to a certain bacterial concentration, it is transferred to the second-level seed tank for cultivation according to a certain inoculation amount, and the first-level seed tank is supplemented with a certain volume of medium for the first-level seed tank to continue the cultivation , according to the growth time of the first-level seed tank, the second-level seed tank inoculation is carried out after reaching the required bacterial concentration, and the continuous cycle is continuously cultivated; in this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.45, and the second-level seed tank is maintained. The OD of the bacteria in the seed tank is 0.9;
[0085] (2) Uninterrupted fermentation: Transfer the bacterial liquid obtained from the cultivation of the secondary seed...
Embodiment 2
[0094] A method for continuously cultivating and producing lysine, comprising the steps of:
[0095] (1) Uninterrupted cultivation: After the first-level seed tank is cultivated to a certain bacterial concentration, it is transferred to the second-level seed tank for cultivation according to a certain inoculation amount, and the first-level seed tank is supplemented with a certain volume of medium for the first-level seed tank to continue the cultivation , according to the growth time of the first-level seed tank, the second-level seed tank inoculation is carried out after reaching the required cell concentration, and the continuous circulation is cultivated successively; in this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.65, and the second-level seed tank is maintained. The OD of the bacteria in the seed tank is 1.1;
[0096] (2) Uninterrupted fermentation: Transfer the bacterial liquid obtained from the cultivation of the secondary see...
Embodiment 3
[0105] A method for continuously cultivating and producing lysine, comprising the steps of:
[0106] (1) Uninterrupted cultivation: After the first-level seed tank is cultivated to a certain bacterial concentration, it is transferred to the second-level seed tank for cultivation according to a certain inoculation amount, and the first-level seed tank is supplemented with a certain volume of medium for the first-level seed tank to continue the cultivation , according to the growth time of the first-level seed tank, the second-level seed tank inoculation is carried out after reaching the required cell concentration, and the continuous circulation is cultivated successively; in this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.5, and the second-level The OD of the bacteria in the seed tank is 1;
[0107] (2) Uninterrupted fermentation: Transfer the bacterial liquid obtained from the cultivation of the secondary seed tank to the first fermente...
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