A desulfurization rhodobacter and its application
A technology of red bacteria and sulfate, applied in bacteria, biological water/sewage treatment, gas fuel, etc., can solve the problem of single nutritional type of strains
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Embodiment 1
[0031] Embodiment 1: strain screening
[0032] Get Chengdu Chang'an Landfill Site (located in Longquanyi District, Chengdu City, Luodai Town, Wanxing Township) leachate is an appropriate amount, and adds in the reactor containing 5L sodium thiosulfate medium by 20% inoculum size, at pH=7.0, 0.5L / min, enrichment culture at 30°C for 7 days, use sodium sulfide medium for plate separation, 3 to 4 days later colonies are formed, pick a single colony and insert it into the plate for culture, and repeat the streak separation more than 4 times to obtain pure bacteria.
Embodiment 2
[0033] Embodiment 2: bacterial strain identification
[0034] The strain was inoculated in sodium thiosulfate liquid medium according to the same inoculum size, and cultured in a shaker at different temperatures (20°C, 25°C, 30°C, 40°C) for 30 h, and the optimum growth temperature of the strain was determined to be 30°C; take initial pH values of 5.0, 6.0, 7.0, 8.0, and 9.0, and culture at the optimum growth temperature for 30 hours to determine the optimum growth pH as 7.0.
[0035] The isolated and purified strains were observed under a scanning electron microscope (SEM), as figure 1 As shown, the bacterium is rod-shaped, 2.0-6.0 μm long, 0.8-1.2 μm wide, without flagella; the Gram test is negative.
[0036] Bacterial whole genome rapid extraction kit was used to extract the whole genome of pure strains, PCR was performed by selecting bacterial 16SrDNA universal primers 27F and 1492R, and then sequenced and analyzed. The sequencing results were compared by BLAST, and the...
Embodiment 3
[0038] Embodiment 3: desulfurization bottle test
[0039] 200ml sodium sulfide medium solution (600mg / L S 2- ) was sterilized under ultraviolet light for 30 minutes, put it into a sterilized 500ml Erlenmeyer flask under aseptic conditions, then added 50ml of Rhodobacter bacteria liquid after inoculation and cultivation for 30 hours, plugged the bottle mouth with an aerobic stopper, and placed it in a shaker Oscillation (30 ℃, 200r / min), measure initial sulfide concentration and be 504mg / L; Described sodium sulfide culture medium formula is: Na 2 S.9H 2 O 10.0g, KH 2 PO 4 1.0g, K 2 HPO 4 1.0g, NH 4 Cl 0.2g, MgCl 2 0.2g, NaHCO 3 1.0g, 1ml of trace element solution, 1ml of vitamin solution, adjust the pH to neutral with 1mol / L HCl solution.
[0040] The same amount of sterile water was added to the control group, and three replicates were set up in each experimental group, and samples were taken every 30 minutes to determine the concentration of sulfide and elemental...
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