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Biological scaffold material suitable for cell growth and preparation method of biological scaffold material

A bio-scaffold and cell technology, applied in the field of bio-scaffold materials and its preparation, can solve problems such as insufficient purity, excessive collagen hydrolysis, and incomplete cell removal, and achieve safe and reliable raw materials, easy industrial production, and good cell compatibility Effect

Active Publication Date: 2017-03-08
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of these problems is not only related to the preparation method, but also related to the control of process conditions. Improper control during the preparation process may lead to excessive collagen hydrolysis, incomplete cell removal, and insufficient purity.

Method used

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  • Biological scaffold material suitable for cell growth and preparation method of biological scaffold material
  • Biological scaffold material suitable for cell growth and preparation method of biological scaffold material
  • Biological scaffold material suitable for cell growth and preparation method of biological scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Sectioning: select the back of fresh pigskin, use a sectioning machine to section, remove the subcutaneous fat tissue of the pigskin, and section the dermis to a thickness of 0.4-0.8mm.

[0048] (2) Water immersion: Weigh 100 parts by weight of animal hide and put it into the drum, add 300 times the volume of purified water, add 4 parts by weight of sodium chloride, rotate for 60-120min, add 50 parts by volume of 95% alcohol , soaked overnight.

[0049] (3) Chemical degreasing: put the washed animal skin into a drum, add 1 part by weight of Pingpingjia, add 1 part by weight of sodium carbonate, and treat at room temperature for 120 minutes. After washing with water, repeat for the second degreasing.

[0050] (4) Alkali erosion-surfactant combination method decellularization: add 1 weight part of sodium hydroxide, 3 weight parts of Pingpingjia, and 300 weight parts of purified water in the drum, and transfer the degreased animal skin to the drum. Drum, rotate 120mi...

Embodiment 2

[0055] (1) Sectioning: select the back of fresh pigskin, use a sectioning machine to section, remove the subcutaneous fat tissue of the pigskin, and section the dermis to a thickness of 0.4-0.8mm.

[0056] (2) Soak in water: take 100 parts by weight of animal hide and put it into the drum, add 400 parts by volume of purified water, add 5 parts by weight of sodium chloride, rotate for 60-120min, add 95% alcohol of 50 parts by volume, Soak overnight.

[0057] (3) Chemical degreasing: put the washed animal skin into a drum, add 2 parts by weight of Pingpingjia, add 1 part by weight of sodium carbonate, and treat at room temperature for 120 minutes. After washing with water, repeat for the second degreasing.

[0058] (4) Alkali erosion-surfactant combination method decellularization: add 1.5 parts by weight of sodium hydroxide, 2 parts by weight of Pingjiao, and 400 parts by weight of purified water in the drum, and transfer the degreased animal skin to the drum. Drum, rotate 12...

Embodiment 3

[0063] (1) Sectioning: select the back of fresh pigskin, use a sectioning machine to section, remove the subcutaneous fat tissue of the pigskin, and section the dermis to a thickness of 0.4-0.8mm.

[0064] (2) Soak in water: take by weighing 100 parts by weight of animal skin and put it into a drum, add 500 parts by volume of purified water, add 10 parts by weight of sodium chloride, rotate for 60-120min, add 95% alcohol of 50 parts by volume, Soak overnight.

[0065] (3) Chemical degreasing: put the washed animal skin into the drum, add 1 part by weight of Pingpingjia, add 2 parts by weight of sodium carbonate, and treat at room temperature for 120 minutes. After washing with water, repeat for the second degreasing.

[0066] (4) Alkali erosion-surfactant combination method decellularization: add 3 parts by weight of potassium hydroxide, 1 part by weight of TritonX-100, purified water of 500 parts by weight in the drum, and transfer the degreased animal skin into the drum, R...

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Abstract

The invention relates to a biological scaffold material suitable for cell growth and a preparation method of the biological scaffold material. The preparation method of the biological scaffold material comprises the following steps: selecting mammal skin after dehairing adopting unhairing equipment in a slaughter house as a raw material, carrying out splitting, carrying out water soaking, carrying out chemical degreasing, carrying out decellularization by adopting an alkali ablation method, carrying out fiber loosening treatment, carrying out freeze drying, and carrying out irradiation sterilization by adopting cobalt-60 and packaging. The prepared biological scaffold material has the three-dimensional porous structure, good hydrophily, high-strength mechanical property, and biocompatibility, and is suitable for the cell adhesion growth. The biological scaffold material can be used for preparing a cell culture scaffold, a tissue filling material, an auxiliary material for treating burns, artificial skin, artificial meniux and a neurolemma sheath.

Description

technical field [0001] The invention relates to a biological scaffold material suitable for cell growth and a preparation method thereof, belonging to the field of tissue engineering scaffold material preparation. Background technique [0002] The acellular dermal matrix prepared from animal skin retains the unique properties of collagen and has a three-dimensional network structure, and has special properties of collagen, such as good cell compatibility and easy binding of required cytokines. (Henson F, Getgood A. The use of scaffolds in musculoskeletal tissue engineering [J]. Open Orthop J, 2011, 5 Suppl 2:261-266.). The clinical application of acellular porcine dermal matrix recorded in the literature is mainly as body surface repair materials, such as artificial skin, wound dressing, etc.; it is also used in tissue filling materials and tissue engineering scaffold materials (Chen Bin, Guo Yongzhang. Tissue engineering skin scaffold Research and application of materials ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/24C08H1/06
Inventor 宋强富勇马文杰
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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