Method for rapid detection of trimethylamine in aquatic product

Abstract

The invention provides a method for rapid detection of trimethylamine in an aquatic product, wherein the method includes the specific steps: preparing trimethylamine standard using gases, introducing into an ion mobility spectrometer, successively detecting, to obtain ion mobility spectrum spectrograms of the trimethylamine standard using gases, and thus obtaining a trimethylamine standard curve; introducing top air formed by a to-be-tested aquatic product into an ion mobility spectrum, to obtain an ion mobility spectrum spectrogram of the to-be-tested sample; and comparing the ion mobility spectrum spectrogram of the top air of the to-be-tested aquatic product with the ion mobility spectrum spectrograms of the standard using gases, and calculating the actual content of trimethylamine in the top air of the to-be-tested aquatic product according to the trimethylamine standard curve. The freshness of the aquatic product is characterized by the measured concentration of volatilized trimethylamine through the method, the identification of the freshness degree of the aquatic product can be effectively achieved, good food quality judgment can be provided for consumers, and food safety is ensured.

Description

technical field [0001] The invention belongs to the field of food, and relates to a technology for rapidly detecting the freshness of aquatic products. Specifically, the invention relates to a method for rapidly detecting the content of trimethylamine in aquatic products. Background technique [0002] Aquatic products such as fish, shrimp, and shellfish are prone to autolysis and spoilage due to high water content and fragile meat tissue during storage and transportation due to the external environment, endogenous enzymes, and microorganisms in the body, causing Protein autolytic degradation, lipid oxidation, and changes in free fatty acids and amino acids produce mixed putrefactive odors. Among them, volatile basic nitrogen substances such as trimethylamine (TMA), dimethylamine (Dimethylamine, DMA) and ammonia formed by the degradation of trimethylamine oxide (TMAO) catalyzed by specific spoilage microbial enzymes have become common at home and abroad. Approved signature f...

AI Technical Summary

Problems solved by technology

Disadvantages of spectrophotometry: Rayleigh's salt and picric acid react with other amines in the sample, often resulting in high measurement results

Disadvantages of gas chromatography: gas chromatography has high sensitivity and fast analysis speed, and is suitable for trace analysis, but low-level aliphatic amines are highly polar and are easily adsorbed on polar stationary phases such as silane and siloxane, resulting in tailing peaks and ghosting, poor repeatability

Disadvantages of gas chromatography-mass spectrometry: SPME requires a special extractor, which is expensive, and the fiber head has a limited service life. It needs to be replaced constantly, which makes the measurement cost higher, and the sample is not easy to store. After extraction, it must be injected and analyzed immediately. , the experimental operation is complicated

Disadvantages of ion chromatography: Since biological samples contain some common cations, such as Ca2+, Na+, K+, Mg2+, considering that these ions will cause great interference to the experiment, ion exchange chromatography is not the best method for measuring amines in biological samples best solution

Disadvantages of capillary electrophoresis: due to the small diameter of the capillary and the short optical path, when using a UV detector, the sensitivity is low, and there are many factors affecting electroosmosis, which in turn affects the repeatability

Disadvantages of mass spectrometry: the sample pretreatment process is complicated, the professional requirements are high, the detection process takes a long time and the efficiency is low

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