Application of monomeric compound in wisteria sinensis sweet caulis and separation and purification method of monomeric compound

A technology for separation and purification of compounds, which is applied in the field of natural plant-derived pesticides and achieves the effects of simple separation and purification methods, good antibacterial activity and good drug safety.

Active Publication Date: 2017-04-05
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the monomeric compounds 3ˊ,5ˊ-dihydroxy-2ˊ,4ˊ,7-trimethoxyisoflavane, 2ˊ,4ˊ,4-trihydroxychalcone, 12-oleanene-3 ,Related research reports on the inhibitory activity of 22,24-triol on fungi in crop hospitals

Method used

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  • Application of monomeric compound in wisteria sinensis sweet caulis and separation and purification method of monomeric compound
  • Application of monomeric compound in wisteria sinensis sweet caulis and separation and purification method of monomeric compound
  • Application of monomeric compound in wisteria sinensis sweet caulis and separation and purification method of monomeric compound

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Take 15kg of dried wisteria medicinal material, pulverize it, and use 80vt% ethanol as a solvent to reflux extract three times at 80°C for 2 hours each time, combine the extracts, recover the extraction solvent, and concentrate to obtain 2kg of extract with a relative density of 1.1 ;

[0047] 2. Gradient elution of the obtained extract with a silica gel column. The eluent is composed of dichloromethane and methanol. The volume ratio of dichloromethane and methanol in the eluent is 100:0~0:1. Gradient elution separation from small to large, TLC detection, 8 components were obtained after merging the same components, which were called Z in order of polarity from small to large 1 -Z 8 ;

[0048] 3. Component Z 4 (82.3g) was eluted through gradient silica gel column, the eluent was composed of petroleum ether and ethyl acetate, the volume ratio of petroleum ether and ethyl acetate in the eluent was 50:1~0:1, the polarity of the eluent Separation by gradient elution ...

Embodiment 2

[0059] 2.1 Instruments and reagents

[0060] 2.1.1 Instruments

[0061] Shimadzu LC-15C high performance liquid chromatography (SPD-15C ultraviolet detector, LC-15C pump, LC-Solution chromatographic workstation, Tianjin Dongkang DT-230A column thermostat), KQ2200 ultrasonic cleaner (Jiangsu Kunshan Ultrasonic Instrument Co., Ltd.), UV759CRT ultraviolet-visible spectrophotometer (Shanghai Youke Instrument Co., Ltd.), German Sartorius CP225D 1 / 100,000 electronic balance.

[0062] 2.1.2 Drug testing

[0063] Acetonitrile and methanol were chromatographically pure (Tianjin Damao Chemical Reagent Factory), water was Wahaha purified water (Hangzhou Wahaha Group Co., Ltd.), and phosphoric acid was analytically pure (Shanghai Suyi Chemical Reagent Co., Ltd.).

[0064] 2.2 Chromatographic analysis conditions

[0065] The experimental chromatographic column is Phenomenex-C 18 (250mm×4.6mm, 5μm), mobile phase is acetonitrile (A)-0.1% phosphoric acid aqueous solution (B), elution cond...

Embodiment 3

[0072] 3.1 Preparation of test sample solution

[0073] Select the isolated compounds 3ˊ,5ˊ-dihydroxy-2ˊ,4ˊ,7-trimethoxyisoflavane, 2ˊ,4ˊ,4-trihydroxychalcone, 12-oleanene-3,22,24- Dissolve an appropriate amount of triol in sterile water (if necessary, add a small amount of DMSO to aid in dissolving), and prepare 0.5 mg·ml respectively -1 , 0.25mg·ml -1 , 0.125mg·ml -1 concentrated drug stock solution.

[0074] 3.2 Preparation of drug-containing medium

[0075] Measure 1mL of each concentration of the test drug solution, filter it through a 0.22μm filter membrane, mix it with 9mL of sterilized PDA medium (potato dextrose agar medium) in a sterile test tube, and pour it into a sterilized petri dish Make a culture medium plate containing medicine, and use sterile water and DMSO as blank control.

[0076] 3.3 Preparation of test pathogenic fungus cake

[0077] Inoculate the four crop pathogenic fungi of Aracea anthracnose, peanut brown spot, eggplant stem blight, and tobacc...

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PUM

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Abstract

The invention discloses an application of a monomeric compound in wisteria sinensis sweet caulis and a separation and purification method of the monomeric compound. The monomeric compound in the wisteria sinensis sweet caulis refers to 3',5'-dihydroxy-2',4',7-dimethoxyisoflavan, 2',4',4-trihydroxychalcone or 12-oleanen-3,22,24-triol. The monomeric compound disclosed by the invention is extracted and separated from the wisteria sinensis sweet caulis and can be used for restraining crop pathogenic fungi.

Description

technical field [0001] The invention relates to the use of a monomer compound in wisteria and its separation and purification method, belonging to the field of natural plant-derived pesticides. Background technique [0002] Botanical pesticides refer to pesticides that use biologically active plants or specific parts thereof, and rough process or extract their active ingredients to make preparations for the prevention and control of harmful organisms. As an important part of biological pesticides, botanical pesticides have the advantages of high efficiency, low toxicity or non-toxicity, low residue, high selectivity, it is difficult for harmful substances to produce resistance to them, and they are easy to mix with other pesticides. With the extensive attention of pesticide research and application departments all over the world, the research and development of new biological source pesticides with high efficiency, low toxicity and easy degradation is the general trend of pe...

Claims

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Application Information

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IPC IPC(8): C07D311/58C07C45/79C07C49/835C07J63/00A01N43/16A01N35/04A01N45/00A01P3/00
CPCA01N35/04A01N43/16A01N45/00C07C45/79C07D311/58C07J63/008C07C49/835Y02A50/30
Inventor 王国凯刘劲松蔡百祥孙云鹏郁阳王诤王刚
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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