Natural fibroin protein fiber scaffold and preparation method

Abstract

The invention relates to a natural fibroin protein fiber scaffold and a preparation method thereof. According to the invention, degumming fibroin protein fiber is dispersed into formic acid to obtain fibroin fiber dispersion liquid; sodium chloride is added in the fibroin fiber dispersion liquid to obtain a fibroin fiber-sodium chloride-formic acid mixing solution; the fibroin fiber-sodium chloride-formic acid mixing solution is volatilized to remove formic acid to obtain a fibroin fiber-sodium chloride solid; the fibroin fiber-sodium chloride-formic acid mixing solution or the fibroin fiber-sodium chloride solid are placed in a flowing water and / or organic solvent to remove sodium chloride to obtain wet fibroin fiber scaffold; and the wet fibroin scaffold is refrigerated and dried to obtain the natural fibroin protein fiber scaffold. The inner structure of the natural fibroin protein fiber scaffold takes fiber as a main part, the natural fibroin protein fiber scaffold has the advantages of high porosity amount, high penetration rate, and excellent mechanical properties, is in favor of nutrients conveying, cell migration, and tissue growth, and is the ideal tissue engineering scaffold.

Description

technical field [0001] The invention relates to a silk fibroin fiber support and a preparation method thereof, which can be used in the fields of regenerative medicine such as repair of soft tissue and hard tissue, drug sustained release, and the like. Background technique [0002] There are millions of patients with organ or tissue damage and functional loss due to diseases and accidents every year. In the United States alone, more than 8 million operations are needed to treat such patients every year, and the economic cost is more than 400 billion US dollars. With the development of modern medicine and surgical techniques, tissue or organ transplantation to repair functional loss has been widely accepted, however, it faces a huge donor gap. The formation of tissues or organs in vivo or in vitro by means of regenerative medicine provides a new treatment option for the repair of damaged functions. Among them, the selection and construction of tissue engineering scaffold mat...

AI Technical Summary

Problems solved by technology

[0004] At present, there are many methods for preparing porous silk fibroin scaffolds, including freeze-drying, salting-out method, gas foaming method, three-dimensional printing, etc. However, these methods still have some insurmountable shortcomings.

For example, the freeze-drying method is easy to form a sheet structure, which is not conducive to the growth of cells and tissues. Although the prior art has reported a method of repeated membrane dissolution to control the self-assembly of silk fibroin to form a nanofibrous structure, and then form a porous scaffold, the efficiency is low. Poor reproducibility, and the existence of pore wall structure directly restricts cell migration and interaction, and tissue growth is thus limited

The fiber scaffold prepared by electrospinning technology is considered to be an ideal scaffold structure for tissue engineering; however, the electrospinning technology is complex in processing, low in yield, and the structure of the electrospun fiber membrane is dense, which is not conducive to the growth of cells and tissues

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