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Preparation method of microbial seaweed fertilizer

A seaweed fertilizer and microorganism technology, applied in magnesium fertilizer, phosphate fertilizer, organic fertilizer and other directions, can solve the problems of difficulty in effectively degrading macromolecular substances in seaweed, destroying nutritional elements of seaweed, and other difficulties, so as to enhance plant stress resistance and reduce pesticides. The use of, the effect of promoting germination and growth

Inactive Publication Date: 2017-04-26
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is still difficult to use microorganisms to fully ferment seaweed to effectively release its nutrients, because seaweed contains a large amount of polysaccharides that are difficult to decompose and high concentrations of salt
At this stage, there are still many defects in the preparation of seaweed fertilizer by fermentation. First, there is a lack of effective strains that can efficiently degrade seaweed polysaccharides and can be directly used as bacterial fertilizers; Degradation of macromolecular substances in seaweed affects the application effect of seaweed fertilizer; third, in order to ensure the fermentation effect, there is a lack of effective pretreatment technology for seaweed raw materials. Pollution

Method used

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  • Preparation method of microbial seaweed fertilizer
  • Preparation method of microbial seaweed fertilizer

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1. A strain of Bacillus licheniformis WS-2, Gram-positive, rod-shaped, with rough colonies, bumps, and irregular edges, can degrade seaweed polysaccharides, secrete a variety of glycosidases and proteases, and have a wide range of antibacterial activities .

[0021] 2. After activating the bacillus in step 1, inoculate in the seaweed culture medium, perform semi-solid fermentation culture to obtain fermentation liquid.

[0022] 3. Centrifuge the fermented liquid obtained in step 2 at 4° C. at 1000 g, and collect the supernatant.

[0023] 4. Filter the supernatant in step 2 with a 0.22 μm filter membrane, collect the filtrate, and obtain a liquid fertilizer containing fucoidan oligosaccharides, that is, a seaweed liquid fertilizer, for use.

[0024] 5. The medium composition of the activated strain in step 2 is: kelp powder 1% of 80 mesh sieves, peptone 0.5%, NaCl 2%, sucrose 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01%, ...

Embodiment 2

[0042] 1. A strain of Bacillus licheniformis WS-2, Gram-positive, rod-shaped, with rough colonies, bumps, and irregular edges, can degrade seaweed polysaccharides, secrete a variety of glycosidases and proteases, and have a wide range of antibacterial activities .

[0043] 2. After activating the bacillus in step 1, inoculate them in equal proportions in seaweed culture medium, and conduct semi-solid fermentation culture to obtain fermentation liquid

[0044] 3. Centrifuge the fermented liquid obtained in step 2 at 4° C. at 1000 g, collect and centrifuge the seaweed fertilizer solids containing active Bacillus amyloliquefaciens WB 1 and Bacillus subtilis ABTNL-2, and dry them at low temperature to obtain seaweed solid fertilizer. Crush and sieve to make powder, set aside.

[0045] 4. The medium composition of the activated strain in step 2 is: kelp powder 1% of 80 mesh sieves, peptone 0.5%, NaCl 2%, sucrose 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, c...

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Abstract

The invention discloses a preparation method of a microbial seaweed fertilizer. Screened Bacillus licheniformis WS-2 having high alginate lyase activity and producing multiple ablastins is adopted to ferment press-shear assisted interaction processed seaweeds to generate a large amount of seaweed oligosaccharides and other micro-molecular special effect metabolites having a promotion effect on the growth of crops, so the bioactivity of the seaweed fertilizer is improved. The seaweed fertilizer shows the multiple crop pathogen inhibition advantage of the Bacillus licheniformis WS-2, has high nutrition added values, improves the properties of soil, and prevents the pesticide and fertilizer integration composite effects of multiple disease pests.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to a preparation method of microbial seaweed fertilizer. Background technique [0002] The long-term and extensive use of chemical fertilizers has gradually caused problems such as soil barrenness, compaction, and nutritional imbalances. The long-term abuse of pesticides has also led to serious problems such as enhanced resistance of pathogenic bacteria and environmental pollution. In order to ensure the high-speed, sustainable and green development of agriculture, the Ministry of Agriculture of my country has paid more and more attention to the use of organic fertilizers, and proposed to gradually reduce the use of chemical fertilizers and pesticides in agriculture. Seaweed is rich in a variety of nutrients and is an excellent raw material for the manufacture of natural organic fertilizers. Its fermented and degraded small molecule oligosaccharides contain natural plant growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05G3/04C05G3/80
CPCC05B7/00C05F11/08C05G3/80C05G5/23C05D3/00C05D5/00C05D9/00C05F11/00C05G3/00
Inventor 李淑英徐牧王熙涛
Owner DALIAN SEM BIOLOGICAL ENG TECH
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