Preparation method of composition containing 2-tripalmitin

A technology of glyceryl palmitate and composition, which is applied in the field of composition preparation, can solve the problems of low product purity, unfavorable environmental protection, and low product purity, and achieve the effect of simple preparation process and few by-products

Inactive Publication Date: 2017-04-26
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these methods are limited by raw materials, and the purity of product is all relatively low
Even if the content of palmitic acid in the 2-position is increased by random transesterification, the purity of the product is still not high due to the limitation of the total composition of palmitic acid
When such a composition is subjected to subsequent refining, a large amount of waste, such as sodium fatty acid, etc., will be generated, which is not conducive to environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Provide a kind of palm oil, its palmitic acid accounts for 21.7% of total fatty acid ratio, and 2 palmitic acid content is 15.6%. Among them, "2-position palmitic acid content" refers to the content of palmitic acid at the 2-position accounting for all the fatty acids at the 2-position, and its detection method refers to GB / T24894, the same below.

[0028] (2) Put 25g of the reaction substrate palm oil, 1g of palmitic acid and 0.5g of potassium hydroxide in a flask, stir and raise the temperature to 170°C and keep it constant, and continue the reaction for 10 hours (transesterification, the same below), then add hexane After 50ml is fully dissolved, the fatty acid potassium is removed by centrifugation to obtain grease A.

[0029] (3) Mix 20g of fat A and 20g of oleic acid, put it in a four-necked flask, stir and heat up to 68°C and keep it constant, add 2.4g of sn-1,3 specific lipase, and reflux for 8 hours (directed transesterification , the same below), to obtai...

Embodiment 2

[0032] (1) Provide a kind of palm oil, its palmitic acid accounts for 22.8% of total fatty acid ratio, and 2 palmitic acid content is 16.4%.

[0033] (2) Add 50 g of palm oil, 1 g of methyl palmitate and 0.5 g of lipase into the flask, stir and raise the temperature to 76° C. and keep it constant, and continue the reaction for 8 hours. After the reaction, fatty acid methyl ester was removed by dry fractionation to obtain fat A.

[0034] (3) Mix 40g of fat A and 20g of linoleic acid, put it in a four-neck flask, stir and raise the temperature to 70°C and keep it constant, add 200ml of cyclohexane, add 2.4g of sn-1,3 specific lipase, and reflux After reacting for 7 hours, mixed oil B was obtained.

[0035] (4) Purify the mixed oil B. The purification steps include but are not limited to: filter to remove lipase, dry fractionation to remove free fatty acids, and obtain a composition containing 2-palmitin glyceride after deodorization, and pass GC and thin layer chromatography T...

Embodiment 3

[0037] Under the same conditions, the products were prepared respectively according to the preparation method of the present invention and the existing preparation method, and compared.

[0038] The method of the invention

[0039] (1) A shortening is provided, the proportion of palmitic acid in the total fatty acids is 23.0%, and the content of 2-position palmitic acid is 16.9%.

[0040] (2) Put 200g of shortening, 200g of ethyl palmitate, 20g of lipase and 1g of deionized water as the reaction substrates in a container, stir and raise the temperature to 60°C and keep it constant, and continue the reaction for 8 hours. After the reaction, fatty acid ethyl ester was removed by molecular distillation to obtain oil A.

[0041] (3) Mix 300g of fat A and 600g of arachidonic acid, put it in a container, stir and raise the temperature to 60°C and keep it constant, add 700ml of octane, add 37g of sn-1,3 specific lipase, and keep the reaction for 8 hours , to get mixed oil B.

[0...

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PUM

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Abstract

The invention relates to a preparation method of a composition containing 2-tripalmitin. The method comprises the following steps: providing glyceride containing palmitic acid residues; carrying out interesterification on the glyceride containing palmitic acid residues and palmitic acid or palmitate salt or non-glyceryl palmitate to obtain grease A; carrying out enzymatic directed interesterification on the grease A and unsaturated fatty acid or nonglyceride of the unsaturated fatty acid to obtain mixed oil B; and purifying the mixed oil B to obtain the composition containing 2-tripalmitin. The preparation method is simple. Compared with existing methods, the method disclosed in the invention has the advantages of high content of 2-tripalmitin in the composition, few byproducts, and facilitation of environmental protection.

Description

technical field [0001] The invention relates to a preparation method of a composition containing 2-glyceryl palmitate. Background technique [0002] Triglycerides are the main fat components ingested by humans. Modern nutrition has confirmed that in the process of digesting triglycerides, the human body will preferentially hydrolyze the fatty acids at the Sn-1 and 3 positions to form 2-monoglycerides and a large amount of free fatty acids. These free fatty acids will undergo two reactions in the body: 1. be absorbed by itself; 2. be excreted after reacting with other substances in the body. Studies have shown that compared with free unsaturated fatty acids, free saturated fatty acids have a slower absorption rate and are more likely to react with calcium to form insoluble soap calcium, which is then excreted from the body. It can be seen that compared with triglycerides that produce free saturated fatty acids, triglycerides that produce free unsaturated fatty acids are mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11C3/10
CPCC11C3/10C12P7/6445
Inventor 汪志明李翔宇陆姝欢周强汪锋肖敏
Owner CABIO BIOTECH WUHAN CO LTD
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