Cell marking probe and preparation method thereof
A technology for labeling probes and probes, which is applied in the field of probes, can solve the problems of difficult formation of cell labeling effect and complex construction, and achieve the effect of inexpensive materials, few operation steps, and simple and convenient preparation process
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[0041] see Figure 5 , Figure 5 It is a flowchart of an embodiment of the preparation method of the cell labeling probe of the present invention, the method includes:
[0042] Step S101: providing nanoparticles or quantum dots, and ascorbyl palmitate, and mixing them uniformly.
[0043] Step S102: adding volatile organic solvent and water to the uniformly mixed mixture, and shaking it evenly.
[0044] Ascorbyl palmitate is insoluble in water and can only be dissolved in organic solvents, such as ethanol, oil, etc. The purpose of the volatile organic solvent is to dissolve the ascorbyl palmitate so that the dissolved ascorbyl palmitate can better wrap or modify the nanoparticles or quantum dots. Subsequent ascorbyl palmitate-coated or modified probes of nanoparticles or quantum dots need to be used for cell labeling, and the organic solvent must be removed to avoid damage to the cells, so water needs to be added, and the organic solvent needs to be a volatile organic solven...
Embodiment 1
[0056] Example 1, construction of ascorbyl palmitate-coated or modified ferric oxide nanoprobes:
[0057] (1) Add ferric oxide nanocrystals with a concentration of 10 mg / ml dispersed in chloroform, n-hexane or dichloromethane into the reagent bottle, and remove the organic solvent by vacuum drying or drying with an inert gas, Then weigh 0.005 parts by weight of nanocrystals;
[0058] (2) 0.010 parts by weight of ascorbyl palmitate and 0.005 parts by weight of the dried iron ferric oxide nanocrystals were shaken and mixed at a temperature of 25-30 degrees Celsius. After mixing, add 1 part by weight of tetrahydrofuran solution;
[0059] (3) Ultrasonic the above-mentioned organic mixed solution at a temperature of 25-30 degrees Celsius, with an ultrasonic power of 130W and an amplitude of 65%, and then add the ultrasonicated mixture into 5 parts by weight of an aqueous solution, and oscillate evenly.
[0060] (4) The residual tetrahydrofuran solvent in the above-mentioned aqueo...
Embodiment 2
[0061] Example 2, construction of ferric oxide / IR780 nanoprobe coated or modified by ascorbyl palmitate:
[0062] (1) Add ferric oxide nanocrystals with a concentration of 10 mg / ml dispersed in chloroform, n-hexane or dichloromethane into the reagent bottle, and remove the organic solvent by vacuum drying or drying with an inert gas, Then weigh 0.005 parts by weight of nanocrystals;
[0063] (2) 0.001 parts by weight of IR780, 0.025 parts by weight of ascorbyl palmitate and 0.005 parts by weight of the above-mentioned dry ferric oxide nanocrystals were shaken and mixed at a temperature of 25-30 degrees Celsius. After mixing, add 1 part by weight of tetrahydrofuran solution;
[0064] (3) Ultrasonic the above-mentioned organic mixed solution at a temperature of 25-30 degrees Celsius, with an ultrasonic power of 130W and an amplitude of 65%, and then add the ultrasonicated mixture into 5 parts by weight of an aqueous solution, and oscillate evenly.
[0065] (4) The residual tet...
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