Method for preparing DL-tryptophan feed additive
A feed additive and tryptophan technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, and animal feed molding or processing, can solve the problems of low fermentation acid production, improve protein synthesis, and improve feed quality. Utilization rate, high effect of animal absorption and utilization rate
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Embodiment 1
[0023] 1. Breeding and biological characteristics of high-producing bacteria of L-tryptophan
[0024] The starting bacteria Corynebacterium glutamicum LZS-1 and LZS-2 were subjected to ultraviolet, sodium nitrite, diethyl sulfate and nitrosoguanidine mutagenesis and a series of primary and secondary screening to obtain acid-producing L-tryptophan The amount of strain was 38.27g / L. The bacterium is tyrosine auxotroph, showing different levels of 6-fluorotryptophan, 5-methyltryptophan, 5-fluorotryptophan, 4-fluorophenylalanine, sulfaguanidine and cinnamic acid resistance.
[0025] (1), UV mutagenesis
[0026] Under the condition of a fixed UV lamp of 40 watts and a distance of 20 cm, the mutagenesis was performed after 60 s of UV irradiation.
[0027] (2), sodium nitrite mutagenesis
[0028] The mutagenesis concentration of immobilized NaNO2 was 0.03mol / L, after 360s of mutagenesis.
[0029] (3) Mutagenesis of diethyl sulfate (DES)
[0030] With 25%Na 2 8 2 O 3 The aque...
Embodiment 2
[0046] The difference from Example 1 is:
[0047] The weight ratio of each component of the seed medium is: glucose 7, ammonium sulfate 0.35, corn steep liquor 5, yeast extract 1.4, magnesium sulfate 0.02, potassium dihydrogen phosphate 0.25, dipotassium hydrogen phosphate 0.15, pH 7.2;
[0048] The weight ratio of each component of the fermentation medium is: sucrose 5, potassium dihydrogen phosphate 0.25, dipotassium hydrogen phosphate 0.13, magnesium sulfate 0.08, ammonium sulfate 1.0, corn steep liquor 5, ferrous sulfate 0.0013, manganese sulfate 0.0013, sulfuric acid Copper 0.0006, L-tyrosine 0.04, thiamine hydrochloride 0.00004, pH 7.2.
Embodiment 3
[0050] The difference from Example 1 is:
[0051] The weight ratio of each component of the seed medium is: glucose 8, ammonium sulfate 0.4, corn steep liquor 6, yeast extract 1.5, magnesium sulfate 0.03, potassium dihydrogen phosphate 0.3, dipotassium hydrogen phosphate 0.2, pH 7.5;
[0052] The weight ratio of each component of the fermentation medium is: sucrose 5.5, potassium dihydrogen phosphate 0.2, dipotassium hydrogen phosphate 0.14, magnesium sulfate 0.10, ammonium sulfate 0.8, corn steep liquor 5.5, ferrous sulfate 0.0014, manganese sulfate 0.0014, sulfuric acid Copper 0.0006, L-tyrosine 0.035, thiamine hydrochloride 0.00004, pH 7.5.
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