Method for simultaneously detecting five triterpene acid components in Cornus officinalis by utilizing UFLC method

A technology of cornus officinalis and triterpenoid acids, which is applied in the field of simultaneous detection of five triterpenoid acids in Cornus officinalis, can solve the problems of long analysis time, difficult separation and the like, and achieves the effects of good separation effect, easy operation, and accurate and reliable measurement results.

Active Publication Date: 2017-05-24
SHAANXI NORMAL UNIV
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  • Abstract
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Problems solved by technology

[0005] At present, the components of triterpene acids in Cornus officinalis are independently tested. Jin Dezhuang et al. used HPLC to analyze the content of ursolic acid and oleanolic acid in Cornus officinalis. Liu Bo et al. used HPLC to detect the content of corosolic acid in Cornus officinalis. Acid and corosolic acid, oleanolic acid and ursolic acid are isomers, these 5 kinds of acids are triterpene acids in Cornus officinalis, their structures and properties are similar, and it takes a long time to analyze them by high performance liquid chromatography And it is not easy to separate, how to quickly and accurately determine the content of maslinic acid, corosolic acid, betulinic acid, oleanolic acid and ursolic acid in Cornus officinalis is of great significance

Method used

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  • Method for simultaneously detecting five triterpene acid components in Cornus officinalis by utilizing UFLC method
  • Method for simultaneously detecting five triterpene acid components in Cornus officinalis by utilizing UFLC method
  • Method for simultaneously detecting five triterpene acid components in Cornus officinalis by utilizing UFLC method

Examples

Experimental program
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Effect test

preparation example Construction

[0041] 2.1 Preparation of mixed reference solution

[0042] Precisely weigh 0.7 mg of ursolic acid, oleanolic acid, maslinic acid, and corosolic acid reference substance, and 1.4 mg of betulinic acid reference substance, dissolve in methanol, and dilute to a 1 mL volumetric flask. Precisely measure 200 μL of each of the above 5 reference solutions and place them in a 1 mL volumetric flask, filter through a 0.22 μm microporous membrane, take the subsequent filtrate, and mix the continuous filtrate to obtain a mixed reference solution.

[0043] 2.2 Preparation of the test solution

[0044] Precisely weigh 1.00g of Cornus officinalis powder, put it in a XO-200 type special glass reaction flask, add 5-25mL of ethanol solution with a volume fraction of 60-90% and seal it, weigh the mass as A, connect the reflux device, and place it in the XO- 200 ultrasonic-microwave combined reaction system for extraction for 6-15min, wherein, microwave power is 100-500W, ultrasonic power is 120-...

Embodiment 1

[0072] Example 1 Selection of solid-liquid ratio

[0073] In this example, five solid-liquid ratios of 1:5, 1:10, 1:15, 1:20, and 1:25 were selected to extract the Cornus officinalis powder respectively, and 1 g of Cornus officinalis powder was taken and placed in an XO-200 type special glass. In the reaction flask, different volumes of 60% ethanol were respectively added, the ultrasonic power was 300w, the temperature was 50°C, the microwave power was 400w, and the extraction time was 5min. Take the supernatant respectively, and use the vanillin-perchloric acid chromogenic method to measure the total triterpene acid content; such as image 3 The results show that the extraction rate increases with the increase of the ratio of solid to liquid at 1:5 to 1:15, and the change of extraction rate decreases when the ratio of solid to liquid is 1:20 to 1:25. In the invention, the ratio of solid to liquid is determined to be 1:15~1:25. Considering the purpose of saving solvent, 1:20 ...

Embodiment 2

[0074] Example 2 Selection of Extraction Time

[0075] In this example, the effect of different extraction times on the extraction rate of total triterpene acids was investigated. 1 g of Cornus officinalis powder was placed in an XO-200 special glass reaction flask, 20 mL of 60% ethanol by volume was added, the ultrasonic power was 300w, the microwave power was 400w, and the temperature 50°C, the extraction time was 3, 6, 9, 12, and 15 min, respectively, and the extraction was performed in sequence according to different extraction times. Take the supernatant respectively, and use the vanillin-perchloric acid chromogenic method to measure the total triterpene acid content; such as Figure 4 As shown, the results show that when the extraction time is 3-9 min, the extraction rate increases sequentially, and when the extraction time is 9-15 min, the extraction rate decreases sequentially. Therefore, in the present invention, the extraction time is determined to be 6 to 15 minute...

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Abstract

The invention relates to a method for simultaneously detecting five triterpene acid components in Cornus officinalis by utilizing a UFLC method. The method comprises the following steps: firstly, configuring reference solutions of ursolic acid, oleanolic acid, maslinic acid, korosorbic acid and betulinic acid respectively; performing chromatographic analysis on the reference solutions to obtain a peak area of each component respectively; using a sample volume of each reference solution as the abscissa and the peak area of each component in the reference solutions as the ordinate to draw a standard curve respectively; adding an extracting agent to Cornus officinalis powder, and extracting to obtain a sample solution; extracting and filtering the sample solution to obtain a filtrate as a test solution; performing chromatographic analysis on the test solution to obtain the peak area of each detected component in the test solution; and calculating to obtain the content of each component, so as to complete the simultaneous detection of five triterpene acid components in Cornus officinalis by utilizing the UFLC method. The method provided by the invention is used for analyzing and reaching components of Cornus officinalis by utilizing the UFLC method, significantly reduces analysis time, can obtain a good separation effect within 9 min, and also reduces the consumption of solvents and samples correspondingly.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of component detection, in particular to a method for simultaneously detecting five triterpene acid components in Cornus officinalis by using a UFLC method. [0003] 【Background technique】 [0004] Cornus offcinalis Sieb.et Zucc is the dried and mature pulp of Cornus offcinalis Sieb.et Zucc, which is sour, astringent, slightly warm in nature, and returns to the liver and kidney meridians. In recent years, Cornus officinalis and its extracts have been shown to have various pharmacological activities, such as anti-inflammatory, antioxidant, anti-aging, hypoglycemic, hypolipidemic, hepatoprotective and neuroprotective effects. The main chemical components in Cornus are iridoid glycosides, tannins, flavonoids, organic acids, polysaccharides, triterpenes, and volatile components. Most of the triterpenoid acids in Cornus belong to the pentacyclic triterpenoids in the triterpenoids. At present, the triterpenoids det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8634
Inventor 康杰芳王艳珍蒋罗曼张秋生王福兴王喆之
Owner SHAANXI NORMAL UNIV
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