Method for preparing silk fibroin nanosphere

A technology of silk fibroin and nanospheres, applied in the field of biomedicine, can solve the problems of high equipment requirements, introduction of organic impurities, low yield, etc., and achieve the effects of improving biological safety, simple and easy-to-obtain equipment, and small environmental impact.

Inactive Publication Date: 2017-05-31
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the high-voltage electrospray method needs to be carried out in a high-voltage electrostatic field, which requires high equipment and low yield; the inverse emulsion method is easy to introd

Method used

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  • Method for preparing silk fibroin nanosphere
  • Method for preparing silk fibroin nanosphere

Examples

Experimental program
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Embodiment 1

[0026] Cut 50g of silkworm cocoons into pieces, put them into 5L boiling aqueous solution of 0.2% anhydrous sodium carbonate, continue to boil for 90 minutes for degumming treatment, filter, collect filter cake, and repeatedly wash with deionized water for 5 times to remove surface residues The sodium carbonate, after drying at 50 ℃, is silk fibroin fiber. Dissolve the dried silk fibroin fibers in 250 mL of a 9.3 mol / L lithium bromide aqueous solution (65°C), and after complete dissolution, put the silk fibroin solution into a dialysis bag (molecular weight cut-off: 3500), and dialyze it in deionized water for 3 days , Change the water twice a day to remove lithium bromide. The silk fibroin aqueous solution obtained after dialysis was adjusted to a mass concentration of 1% with deionized water.

[0027] Anhydrous ethanol was pre-cooled in a refrigerator at 4°C for 1 hour. Take 10 mL of absolute ethanol and add it to 100 mL of silk fibroin aqueous solution with a mass concent...

Embodiment 2

[0029] Cut 50g of tussah cocoons into pieces, put them into 5L of boiling mass concentration 0.2% anhydrous sodium carbonate aqueous solution, continue to boil for 120 minutes to carry out degumming treatment, filter, collect filter cake, and repeatedly wash the obtained silk fibroin fibers with deionized water 5 After removing the residual sodium carbonate on the surface, and drying at 50 °C, it is silk fibroin fiber. Dissolve the dried silk fibroin fibers in 500 ml of a ternary solution at 70°C (the volume ratio of calcium chloride / ethanol / water is 1:2:8), and after complete dissolution, put the silk fibroin solution into a dialysis bag ( Molecular weight cut-off: 3500), dialyzed in deionized water for 4 days, and changed the water twice a day to remove residual solvent. The silk fibroin aqueous solution obtained after dialysis was adjusted to a mass concentration of 5% with deionized water.

[0030] 5 μg of bFGF-2 was dissolved in 100 mL of silk fibroin aqueous solution wi...

Embodiment 3

[0033] Put 50g of silkworm raw silk into 5L of boiling 0.2% anhydrous sodium carbonate aqueous solution, continue to boil for 60 minutes to carry out degumming treatment, filter, take filter cake, repeatedly wash with deionized water and deionized water for 5 times to remove surface residues The sodium carbonate, after drying at 50 ℃, is silk fibroin fiber. Dissolve the dried silk fibroin fibers in 500 ml of a ternary solution at 70°C (the volume ratio of calcium chloride / ethanol / water is 1:2:8), and after complete dissolution, put the silk fibroin solution into a dialysis bag ( Molecular weight cut-off: 3500), dialyzed in deionized water for 4 days, and changed the water twice a day to remove residual solvent. The silk fibroin aqueous solution obtained after dialysis was adjusted to a mass concentration of 2% with deionized water.

[0034] Dissolve 1 μg of BMP-2 in a 2% mass concentration, 100 mL silk fibroin aqueous solution, and mix at 4° C. and 30 rpm for 30 minutes to ob...

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Abstract

The invention discloses a method for preparing silk fibroin nanospheres. The method comprises the following steps: degumming mulberry silk or tussah silk so as to obtain silk fibroin, dissolving and dialyzing the silk fibroin so as to form a silk fibroin solution of certain concentration, uniformly mixing absolute ethyl alcohol with the silk fibroin solution in a certain ratio, freezing, centrifuging, washing, and drying, thereby obtaining the silk fibroin nanospheres. Functional silk fibroin microspheres can be prepared by mixing the silk fibroin solution with functional protein molecules such as bFGF-2, IGF-1, EGF, BMP-2 and OP-1. The method is safe, simple and convenient in preparation process and small in pollution, and the silk fibroin nanospheres which are uniform in particle size can be stably prepared, are applicable to injection, and can be applied to fields such as medicine carriers and slow-release systems.

Description

[0001] (1) Technical field [0002] The invention relates to a preparation method of silk fibroin-based nanospheres suitable for controlling drug sustained release, and belongs to the technical field of biomedicine. [0003] (2) Background technology [0004] At present, from the perspective of the development trend of biomedical materials, synthetic polymers and other non-degradable materials or materials with poor biocompatibility of degradation products will gradually decrease, while natural materials with good biocompatibility and in vivo absorbability will gradually decrease. Biomaterials will be favored. Natural biomaterials have great potential for clinical applications with controllable structural, mechanical, and biodegradable properties. [0005] Among many natural biological materials, silk fibroin derived from natural silk has been widely used in the field of tissue engineering in recent years due to its advantages of easy processing, good biocompatibility, high me...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/56A61L27/58A61K9/51A61K47/42A61K38/18A61K31/704A61K31/7068
CPCA61L27/227A61K9/0019A61K9/5169A61K31/704A61K31/7068A61K38/18A61K38/1808A61K38/1825A61K38/1841A61K38/1875A61K47/42A61L27/56A61L27/58A61L2300/604A61L2300/624A61L2400/12A61L2430/34
Inventor 汪燕艳邹晓晖赵洪石
Owner ZHEJIANG UNIV
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