Narrow-distributed hydroxyethyl starch and application thereof
A hydroxyethyl starch, narrow distribution technology, used in the field of medicine, can solve the problems of cell toxicity, affecting cell activity, and poor cell status.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Prepare a cornstarch suspension with a concentration of 15% (w / v), gelatinize at 95°C for 30min, adjust the acidity of the system to 0.5mol / L with hydrochloric acid, set the temperature at 60°C, and react for 4 hours to obtain starch hydrolyzate; starch hydrolyzate The concentrated solution is obtained by separating the ultrafiltration membrane with a molecular weight cut-off of 50,000Da; 2 Carry out hydroxyethyl substitution reaction with oxirane under protection under sodium hydroxide catalysis, wherein starch hydrolyzate: sodium hydroxide: the reaction molar ratio of chloroethanol is 1:0.8:0.5, and the reaction time is 10 hours, and reaction temperature The temperature is 35°C; the reaction solution is decolorized by activated carbon, filtered and then spray-dried to obtain a powder product of narrowly distributed hydroxyethyl starch. According to the method of Ph.Eur.2.2.30and monograph (01 / 2011:1785), the weight-average molecular mass Mw of the product is 2.883e+5,...
Embodiment 2
[0031] Taking the commercially available Ficoll-sodium diatrizoate separating liquid as the control sample, respectively, with the narrow distribution hydroxyethyl starch, hydroxyethyl starch 130 / 0.4, hydroxyethyl starch 200 / 0.5 and hydroxyethyl starch 480 / The medicine of 0.7 is a test sample.
[0032] (i) Take fresh anticoagulated blood, carefully add it to the upper layer of Ficoll-sodium diatrizoate separation solution according to the volume 1:1, and centrifuge at 500g for 30 minutes at room temperature (18-22°C). Cells are divided into four layers, such as figure 2 shown. The first layer is the plasma layer (containing platelets). The second layer is a ring of opalescent lymphocytes (monocyte / mononuclear cell layer). The third layer is a transparent separation liquid layer. The fourth layer is the red blood cell layer (containing a large number of neutrophils). Collect the cells and put them into a test tube containing 10 mL of washing solution, mix thoroughly, and...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com